Description: As part of the Selected Methods in Enzymology series, this book provides a methodological survey on biochemical techniques collected during the past two decades. The book, which condenses 24 volumes of the series into one, provides reedited and technically updated versions of central aspects for the experimental approaches to the description of structure and function of bacterial and eukaryotic (plant and animal) cell membranes and membranous organelles.
Purpose: This volume collects key methods of the field and also includes a historical perspective. Some techniques are replaceable by more recent approaches, based on technical developments of the past decade, but some aspects of well-established techniques are irreplaceable.
Audience: The book is written for anyone doing active research in the biology and chemistry of membranes, cellular membranes, and cell compartments. Just because this issue contains some "classics" of experimental biology, it may be suited also for high schools fostering experimental activities in teaching biology.
Features: The style of presentation is typical for Methods in Enzymology books, as a laboratory manual in paperback format. However, it is unlikely that laboratory personnel will use the book as an immediate source for procedures, because it contains too many scientific comments and results. The large number of up-to-date references and a detailed index makes it a profound source of first-hand information.
Assessment: This book is review on an entire field of experimental knowledge and compendium of historic development for biochemistry (rather than nucleic acid research) as the central domain of biomedicine.