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Biomembranes

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This volume contains papers selected from the twenty-four Methods in Enzymology volumes on Biomembranes. The articles were chosen based on their impact on the field and how well the methods they cover have withstood the test of time. They represent a milestone in our ...
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Overview

This volume contains papers selected from the twenty-four Methods in Enzymology volumes on Biomembranes. The articles were chosen based on their impact on the field and how well the methods they cover have withstood the test of time. They represent a milestone in our understanding by the giants in the field.

Key Features
* Purification, functional characterization, reconstitution, and organization
* Transmembrane and associated proteins
* Membrane lipids and lipoproteins
* Bioenergetics and transport
* Biogenesis, targeting, and assembly

Audience: Biochemists, biophysicists, molecular and cell biologists, physiologists, microbiologists, pharmacologists, analytical chemists, and biomedical researchers. Professionals and students.

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Editorial Reviews

Doody's Review Service
Reviewer: Juergen Mollenhauer, PhD, DSc (Rush Medical College of Rush University)
Description: As part of the Selected Methods in Enzymology series, this book provides a methodological survey on biochemical techniques collected during the past two decades. The book, which condenses 24 volumes of the series into one, provides reedited and technically updated versions of central aspects for the experimental approaches to the description of structure and function of bacterial and eukaryotic (plant and animal) cell membranes and membranous organelles.
Purpose: This volume collects key methods of the field and also includes a historical perspective. Some techniques are replaceable by more recent approaches, based on technical developments of the past decade, but some aspects of well-established techniques are irreplaceable.
Audience: The book is written for anyone doing active research in the biology and chemistry of membranes, cellular membranes, and cell compartments. Just because this issue contains some "classics" of experimental biology, it may be suited also for high schools fostering experimental activities in teaching biology.
Features: The style of presentation is typical for Methods in Enzymology books, as a laboratory manual in paperback format. However, it is unlikely that laboratory personnel will use the book as an immediate source for procedures, because it contains too many scientific comments and results. The large number of up-to-date references and a detailed index makes it a profound source of first-hand information.
Assessment: This book is review on an entire field of experimental knowledge and compendium of historic development for biochemistry (rather than nucleic acid research) as the central domain of biomedicine.
Juergen Mollenhauer
As part of the Selected Methods in Enzymology series, this book provides a methodological survey on biochemical techniques collected during the past two decades. The book, which condenses 24 volumes of the series into one, provides reedited and technically updated versions of central aspects for the experimental approaches to the description of structure and function of bacterial and eukaryotic (plant and animal) cell membranes and membranous organelles. "This volume collects key methods of the field and also includes a historical perspective. Some techniques are replaceable by more recent approaches, based on technical developments of the past decade, but some aspects of well-established techniques are irreplaceable. "The book is written for anyone doing active research in the biology and chemistry of membranes, cellular membranes, and cell compartments. Just because this issue contains some classics of experimental biology, it may be suited also for high schools fostering experimental activities in teaching biology. "The style of presentation is typical for Methods in Enzymology books, as a laboratory manual in paperback format. However, it is unlikely that laboratory personnel will use the book as an immediate source for procedures, because it contains too many scientific comments and results. The large number of up-to-date references and a detailed index makes it a profound source of first-hand information. "This book is review on an entire field of experimental knowledge and compendium of historic development for biochemistry (rather than nucleic acid research) as the central domain of biomedicine.

3 Stars from Doody
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Product Details

  • ISBN-13: 9780125435659
  • Publisher: Elsevier Science & Technology Books
  • Publication date: 12/15/1996
  • Series: Selected Methods in Enzymology Series
  • Edition number: 1
  • Pages: 1096
  • Product dimensions: 7.59 (w) x 9.65 (h) x 1.94 (d)

Table of Contents

Purification, Functional Characterization, Reconstitution, and Organization:
Animals:
S. Fleischer and M. Kervina, Long-Term Preservation of Liver for Subcellular Fractionation.
S. Fleischer and M. Kervina, Subcellular Fraction of Rat Liver.
H. Sies, The Use of Perfusion of Liver and Other Organs for the Study of Microsomal Electron-Transport and Cytochrome P-450 Systems.
P. Moldeus, J. Hogberg, and S. Orrenius, Isolation and Use of Liver Cells.
G. Dallner, Isolation of Rough and Smooth Microsomes--General.
B. Fleischer, Isolation and Characterization of Golgi Apparatus and Membranes from Rat Liver.
P. Baudhuin, Isolation of Rat Liver Peroxisomes.
A. Trouet, Isolation of Modified Liver Lysosomes.
T.L. Steck and J.A. Kant, Preparation of Impermeable Ghosts and Inside-Out Vesicles from Human Erythrocyte Membranes.
B. Cannon and O. Lindberg, Mitochondria from Brown Adipose Tissue: Isolation and Properties.
J.W. Greenwalt, Survey and Update of Outer and Inner Mitochondrial Membrane Separation.
C.-P. Lee, Tightly Coupled Beef Heart Submitochondrial Particles.
K. van Dam and A.H.C. Wiechmann, Respiratory Control and Oxidative Phosphorylation Measurements in Mitochondria.
D.S. Papermaster, Preparation of Retinal Rod Outer Segments.
P.A. Fisher and G. Blobel, Preparation of a Nuclear Matrix-Pore Complex--Lamina Fraction from Embryos of Drosophila melanogaster. Plants:
D.A. Walker, Z.G. Cerovic, and S.P. Robinson, Isolation of Intact Chloroplasts: General Principles and Criteria of Integrity.
R. Douce, J. Bourguignon, R. Brouquisse, and M. Neuburger, Isolation of Plant Mitochondria: General Principles and Criteria of Integrity.
E.L. Vigil, T.K. Fang, and R.P. Donaldson, Isolation of Glyoxysomes and Purification of Glyoxysomal Membranes.
D.P. Briskin, R.T. Leonard, and T.K. Hodges, Isolation of the Plasma Membrane: Membrane Markers and General Principles.
Bacteria and Unicellular Eukaryotes:
B. Guerin, P. Labbe, and M. Somlo, Preparation of Yeast Mitochondria (Saccharomyces cerevisiae) with Good P/O and Respiratory Control Ratios.
D. Oesterhelt and W. Stoeckenius, Isolation of the Cell Membrane of Halobacterium halobium and Its Fractionation into Red and Purple Membrane.
M.R.J. Salton, Isolation of Cell Walls from Gram-Positive Bacteria.
A.E. Walsby, The Isolation of Gas Vesicles from Blue-Green Algae.
Specialized Enzymes, Assays, and Reconstitution Methodology:
G. Meissner and S. Fleischer, Reconstitution of Functional Sarcoplasmic Reticulum Membrane Vesicles.
J.D. Crapo, J.M. McCord, and I. Fridovich, Preparation and Assay of Superoxide Dismutases.
H. Beinert, EPR Spectroscopy of Components of the Mitochondrial Electron-Transfer System.
R.W. Estabrook and J. Werringloer, The Measurement of Difference Spectra: Application to the Cytochromes of Microsomes.
C.S. Lieber,L.M. DeCarli, S. Matsuzaki, K. Ohnishi, and R. Teschke, The Microsomal Ethanol Oxidizing System (MEOS).
J.-A. Gustafsson, Steroid Hydroxylations Catalyzed by Cytochrome P-450.
H. Bayley, B. Hojeberg, K.-S. Huang, H.G. Khorana, M.-J. Liao, C. Lind, and E. London, Delipidation, Renaturation, and Reconstitution of Bacteriorhodopsin.
P. Baudhuin, Morphometry of Subcellular Fractions.
K. Fisher and D. Branton, Application of the Freeze-Fracture Technique to Natural Membranes.
B.F.van Gelder, Optical Properties of Cytochromes from Beef Heart Mitochondria, Submitochondrial Vesicles, and Derived Preparations.
Membrane Lipids and Lipoproteins:
R.S. Kaplan and P.L. Pedersen, Sensitive Protein Assay in Presence of High Levels of Lipid.
H.H. Kamp and K.W.A. Wirtz, Phosphatidylcholine Exchange Protein from Beef Liver.
J.A. Buege and S.D. Aust, Microsomal Lipid Peroxidation.
A.L. Tappel, Gluathione Peroxidase and Hydroperoxides.
M.C. Woodle and D. Papahadjopoulous, Liposome Preparation and Size Characterization.
Biogenesis, Targeting, and Assembly:
P.M. Litzardi, Methods for the Preparation of Messenger RNA.
P. Walter and G. Blobel, Preparation of Microsomal Membranes for Cotranslational Protein Translocation.
R.C. Jackson, Quantitative Assay for Signal Peptidase.
H. Freitag, R. Benz, and W. Neupert, Isolation and Properties of the Porin of the Outer Mitochondrial Membrane from Neurospora crassa.
S.M. Gasser, Import of Polypeptides into Isolated Yeast Mitochondria.
C.L. Dieckmann and A. Tzagoloff, Analysis of Yeast Mitochondrial Genes.
G. Attardi and J. Montoya, Analysis of Human Mitochondrial RNA.
J.E. Mullet and N.-H. Chua, In Vitro Reconstitution of Synthesis, Uptake, and Assembly of Cytoplasmically Synthesized Chloroplast Proteins.
J.L. Goldstein, S.K. Basu, and M.S. Brown, Receptor-Mediated Endocytosis of Low-Density Lipoprotein in Cultured Cells.
M. Marsh, A. Helenius, K. Matlin, and K. Simons, Binding, Endocytosis, and Degradation of Enveloped Animal Viruses.
Bioenergetics and Transport:
G.F.-L. Ames, Isolation of Transport Mutants in Bacteria.
Y. Hatefi, Reconstitution of the Electron-Transport System of Bovine Heart Mytochondria.
P.G. Heytler, Uncouplers of Oxidative Phosphorylation.
H. Rottenberg, The Measurement of Membrane Potential and delta pH in Cells, Organelles, and Vesicles.
P. Mitchell, J. Moyle, and R. Mitchell, Measurement of (H+/O in Mitochondria and Submitochondrial Vesicles.
B. Reynafarje, M.D. Brand, A. Alexandre, and A.L. Lehninger, Determination of the H+/Site and Ca2+/Site Ratios of Mitochondrial Electron Transport.
F. Palmieri and M. Klingenberg, Direct Methods for Measuring Metabolite Transport and Distribution in Mitochondria.
J.J. Lemasters and C.R. Hackenbrock, Continuous Measurement of Adenosine Triphosphate with Firefly Luciferase Luminescence.
M. Klingenberg, Fluorescent Nucleotide Analogs as Active Site Probes for the ADP/ATP Carrier and the Uncoupling Protein.
H.W. Heldt and U.I. Flugge, Transport of Metabolites across the Chloroplast Envelope.
U. Pick, Isolation and Reconstitution of CF0-F1 from Chloroplasts.
M.S. Braiman, Resonance Raman Methods for Proton Translocation in Bacteriorhodopsin.
R.J. Mehlhorn, L. Packer, R. Macey, A.T. Balaban, and I. Dragutan, Measurement of Transmembrane Proton Movements with Nitroxide Spin Probes.
M. Klingenberg and E. Winkler, Reconstitution of an H+ Translocator, the Uncoupling Protein from Brown Adipose Tissue Mitochondria, in Phospholipid Vesicles.
P.L. Jorgensen, Purification of Na+, K+-ATPase: Enzyme Sources, Preparative Problems, and Preparation from Mammalian Kidney.
S.J.D. Karlish, Measurement of Active and Passive Na+ and K+ Fluxes in Reconstituted Vesicles.
A. Chu, M.C. Dixon, A. Saito, S. Seiler, and S. Fleischer, Isolation of Sarcoplasmic Reticulum Fractions Referable to Longitudinal Tubules and Junctional Terminal Cisternae from Rabbit Skeletal Muscle.
B.K. Chamberlain and S. Fleischer, Isolation of Canine Cardiac Sarcoplasmic Reticulum.
M. Inui and S. Fleischer, Reconstitution of Calcium Pumping of Cardiac Sarcoplasmic Reticulum.
J.T. Penniston, A.G. Filoteo, C.S. McDonough, and E. Carafoli, Purification, Reconstitution, and Regulation of Plasma Membrane Ca2+-Pumps.
M. Inui and S. Fleischer, Purification of Ca2+ Release Channel (Ryanodine Receptor) from Heart and Skeletal Muscle Sarcoplasmic Reticulum.
E.J. Bowman and B.J. Bowman, Purification of Vacuolar Membranes, Mitochondria, and Plasma Membranes from Neurospora crassa and Modes of Discriminating among the Different H+-ATPases.
N. Nelson, S. Cidon, and Y. Moriyama, Chromaffin Granule Proton Pump.
X.-S. Xie, D.K. Stone, and E. Racker, Proton Pump of Clathrin-Coated Vesicles.
E.C. Rabon, W.B. Im, and G. Sachs, Preparation of Gastric H+, K+-ATPase.
R. Tsien and T. Pozzan, Measurement of Cytosolic Free Ca2+ with Quin2.
J.R. Sachs, Cation Fluxes in the Red Blood Cell: Na+, K+ Pump.
W.R. Widdas, Sugar Transport in Red Blood Cells.
T.P.M. Akerboom and H. Sies, Transport of Glutathione, Glutathione Disulfide, and Glutathione Conjugates across the Hepatocyte Plasma Membrane.
P.E. Kish and T. Ueda, Glutamate Accumulation into Synaptic Vesicles.
N.-E.L.Saris and A. Allshire, Calcium Ion Transport in Mitochondria.
D.W. Landry, M.A. Akabas, C. Redhead, and Q. Al-Awqati, Purification and Reconstitution of Epithelial Chloride Channels.
W.W. Reenstra and J.G. Forte, Isolation of H+, K+-ATPase-Containing Membranes from the Gastric Oxyntic Cell.
O.H. Peterson, Patch-Clamp Experiments in Epithelia: Activation by Hormones or Neurotransmitters.
K.J. Ullrich, Epithelial Transport: An Introduction.
Subject Index.
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