Bioprocess Engineering: Basic Concepts / Edition 3

by Michael Shuler, Fikret Kargi, Matthew DeLisa

Overview

The Leading Introduction to Biochemical and Bioprocess Engineering, Updated with Key Advances in Productivity, Innovation, and Safety

Bioprocess Engineering, Third Edition, is an extensive update of the world’s leading introductory textbook on biochemical and bioprocess engineering and reflects key advances in productivity, innovation, and safety.

The authors review relevant fundamentals of biochemistry, microbiology, and molecular biology, including enzymes, cell functions and growth, major metabolic pathways, alteration of cellular information, and other key topics. They then introduce evolving biological tools for manipulating cell biology more effectively and to reduce costs of bioprocesses.

This edition presents major advances in the production of biologicals; highly productive techniques for making heterologous proteins; new commercial applications for both animal and plant cell cultures; key improvements in recombinant DNA microbe engineering; techniques for more consistent authentic post-translational processing of proteins; and other advanced topics. It includes new, improved, or expanded coverage of

• The role of small RNAs as regulators
• Transcription, translation, regulation, and differences between prokaryotes and eukaryotes
• Cell-free processes, metabolic engineering, and protein engineering
• Biofuels and energy, including coordinated enzyme systems, mixed-inhibition and enzyme-activation kinetics, and two-phase enzymatic reactions
• Synthetic biology
• The growing role of genomics and epigenomics Population balances and the Gompetz equation for batch growth and product formation
• Microreactors for scale-up/scale-down, including rapid scale-up of vaccine production
• The development of single-use technology in bioprocesses
• Stem cell technology and utilization
• Use of microfabrication, nanobiotechnology, and 3D printing techniques
• Advances in animal and plant cell biotechnology

The text makes extensive use of illustrations, examples, and problems, and contains references for further reading as well as a detailed appendix describing traditional bioprocesses.

Product Details

ISBN-13:	9780137062706
Publisher:	Pearson Education
Publication date:	03/30/2017
Edition description:	New Edition
Pages:	656
Product dimensions:	7.40(w) x 9.20(h) x 1.50(d)

About the Author

Dr. Michael L. Shuler is Samuel B. Eckert Professor of Engineering at Cornell University. He directed the School of Chemical Engineering (1998-2002) and was founding James and Marsha McCormick Chair for Biomedical Engineering (2004-2014). He also directs the Center on the Microenvironment and Metastasis (CMM), funded by the National Cancer Institute as a Physical Sciences - Oncology Center. He has received numerous teaching, advising, and research related awards, and has been elected to the National Academy of Engineering and the American Academy of Arts and Sciences.

Fikret Kargi is Professor in the Department of Environmental Engineering at Dokuz Eylul University. His interests include bioprocess engineering, environmental biotechnology, wastewater treatment, biotechnology-bioengineering, and waste bioprocessing. He holds a Ph.D. in Chemical/Biochemical Engineering from Cornell.

Matthew DeLisa is William L. Lewis Professor of Engineering in Cornell's Department of Chemical and Biomolecular Engineering. His research focuses on understanding and controlling the molecular mechanisms underlying protein biogenesis in the complex environment of a living cell. He has invented numerous commercially important technologies for facilitating the discovery, design and manufacturing of human drugs, and has made seminal discoveries about cellular protein folding and protein translocation. DeLisa has received several awards including an NSF CAREER award, and was named one of the top 35 young innovators by MIT's Technology Review. He was elected as a fellow of the American Institute for Medical and Biological Engineering in 2014.

Table of Contents

• Part I: The Basics of Biology: An Engineer’s Perspective
• Chapter 1: What Is a Bioprocess Engineer?
• Chapter 2: An Overview of Biological Basics
• Chapter 3: Enzymes
• Chapter 4: How Cells Work
• Chapter 5: Major Metabolic Pathways
• Chapter 6: How Cells Grow
• Chapter 7: Stoichiometry of Microbial Growth and Product Formation
• Chapter 8: How Cellular Information Is Altered
• Part II: Engineering Principles for Bioprocesses
• Chapter 9: Operating Considerations for Bioreactors for Suspension and Immobilized Cultures
• Chapter 10: Selection, Scale-Up, Operation, and Control of Bioreactors
• Chapter 11: Recovery and Purification of Products
• Chapter 12: Bioprocess Considerations in Using Animal Cell Cultures
• Chapter 13: Bioprocess Considerations in Using Plant Cell Cultures
• Chapter 14: Utilizing Genetically Engineered Organisms
• Chapter 15: Medical Applications of Bioprocess Engineering
• Chapter 16: Multiple Species in Culture
• Appendix A: Traditional Industrial Bioprocesses

Preface

In the decade since the first edition of Bioprocess Engineering: Basic Concepts, biotechnology has undergone several revolutions. Currently, the ability to sequence the genome of whole organisms presents opportunities that could be hardly envisioned ten years ago. Many other technological advances have occurred that provide bioprocess engineers with new tools to serve society better. However, the principles of bioprocess engineering stated in the first edition remain sound.

The goals of this revision are threefold. We want to capture for students the excitement created by these advances in biology and biotechnology. We want to inform students about these tools. Most importantly, we want to demonstrate how the principles of bioprocess engineering can be applied in concert with these advances.

This edition contains a new section in the first chapter alerting students to the regulatory issues that constrain bioprocess design and modification. We believe students need to be aware of these industrially critical issues. Part 2, "An Overview of Biological Basics," has been updated throughout and expanded. Greater emphasis is given now to posttranslational processing of proteins, as this is a key issue in choice of bioprocessing strategies to make therapeutic proteins. Basic processes in animal cells are more completely described, since animal cell culture is now an established commercial bioprocess technology. Chapter 5 is made more complete by introduction of a section on noncarbohydrate metabolism. Key concepts in functional genomics have been added to prepare students to understand the impact of these emerging ideas and technologies on bioprocesses.

In Part 3, "Engineering Principles for Bioprocesses," greater attention is given to issues associated with animal cell bioreactors. The discussion of chromatographic processes is expanded. In Part 4, "Applications to Nonconventional Biological Systems," the material has been rearranged and updated and a new chapter added. These changes are evident in the chapters on animal and plant cell culture. Particularly important is the expanded discussion on choice of host-vector systems for production of proteins from recombinant DNA technology. Coverage of two areas of increasing importance to bioprocess engineers, metabolic and protein engineering, has been expanded. A new chapter on biomedical applications illustrates how approaches to bioprocess engineering are relevant to problems typically considered to be biomedical engineering. The chapter on mixed cultures has been extended to cover advanced waste-water treatment processes. An appendix providing descriptive overviews of some traditional bioprocesses is now included.

The suggestions for further reading at the end of each chapter have been updated. We are unable in this book to provide in-depth treatment of many vital topics. These readings give students an easy way to begin to learn more about these topics.

Teaching a subject as broad as bioprocess engineering in the typical one-semester, three-credit class has never been easy. Although some material in the first edition has been removed or condensed, the second edition is longer than the first. For students with no formal background in biology, coverage of all of the material in this book would require a four-credit class. In a three-credit class we suggest that the instructor cover Chapters 1 to 11 (with 7 being optional) and then decide on subsequent chapters based on course goals. A course oriented toward biopharmaceuticals will want to include careful coverage of Chapters 12 and 14 and some coverage of 13 and 15. A course oriented toward utilization of bioresources would emphasize Chapter 16 and the Appendix and selected coverage of topics in Chapters 13 and 14.

Many students now enter a bioprocess engineering course with formal, college-level instruction in biology and biochemistry. For such students Chapters 2, 4, 5, 7, and 8 can be given as reading assignments to refresh their memories and to insure a uniform, minimal level of biological knowledge. Lecture time can be reserved for material in other chapters or for supplementary material. For these five chapters study questions are provided for self-testing. Under these circumstances the instructor should be able to cover the rest of the material in the book.