Bioremediation: Methods and Protocols / Edition 1

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Since its first systematic application during the 1970s, bioremediation, or the exploitation of a biological system's degradative potential to combat toxic pollutants such as heavy metals, polyaromatic hydrocarbons (PAH), cyanides, and radioactive material, has proven itself over time, and the many advances in molecular techniques have only amplified its utility. In Bioremediation: Methods and Protocols, experts in the field explore imaginative and ambitious multidisciplinary techniques that will enable more predictable removal of pollutants from a variety of environments. The easy-to follow volume addresses some of the broader issues such as the effect of the environment in determining the availability and fate of organic and inorganic compounds and how choices around the most appropriate bioremediation process can be arrived at, as well as detailed complementary techniques that support the effective deployment and monitoring of a bioremediation approach. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioremediation: Methods and Protocols offers researchers a series of invaluable techniques certain to enhance their work with environmental contamination.

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Editorial Reviews

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From the reviews:

“Bioremediation is an area with growing importance … and the publication of this book timely. This book provides an array of molecular applications in the fields of bioremediation and microbial ecology. It is both informative and practical, the examples chosen are highly relevant and the prools clearly written. It is a very useful handbook for microbiologists and environmental professionals with special interests in bioremediation. I also find this book a good source of inspiration for devising projects for our final year undergraduate and postgraduate students.” (Diane Purchase, Microbiology Today, June, 2010)

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Product Details

  • ISBN-13: 9781607614388
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/16/2009
  • Series: Methods in Molecular Biology Series, #599
  • Edition description: 2010
  • Edition number: 1
  • Pages: 285
  • Product dimensions: 7.10 (w) x 10.10 (h) x 0.90 (d)

Table of Contents

Preface v

Contributors ix

1 Measurement of Bioaccessibility of Organic Pollutants in Soil Laura McAllister Kirk T. Semple 1

2 Heavy Metal Bioavailability and Bioaccessibility in Soil John Richard Dean 15

3 Addressing PCR Biases in Environmental Microbiology Studies Rita Sipos Anna Székely Sára Révész Károly Márialigeti 37

4 MPN- and Real-Time-Based PCR Methods for the Quantification of Alkane Monooxygenase Homologous Genes (alkB) in Environmental Samples Alfredo Pérez-de-Mora Stephan Schulz Michael Schloter 59

5 Application of Fingerprinting Molecular Methods in Bioremediation Studies Dimitrios G. Karpouzas Brajesh K. Singh 69

6 Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Assess Microbial Community Structure in Compost Systems Sonia M. Tiquia 89

7 Combination of Fluorescence In Situ Hybridization with Staining Techniques for Cell Viability and Accumulation of PHA and polyp in Microorganisms in Complex Microbial Systems Jeppe Lund Nielsen Caroline Kragelund Per Halkjar Nielsen 103

8 Ecophysiological Analysis of Microorganisms in Complex Microbial Systems by Combination of Fluorescence In Situ Hybridization with Extracellular Staining Techniques Jeppe Lund Nielsen Caroline Kragelund Per Halkjar Nielsen 117

9 Applications of DNA-Stable Isotope Probing in Bioremediation Studies Yin Chen Jyotsna Vohra J. Colin Murrell 129

10 Two-Dimensional Gel Electrophoresis: Discovering Biomolecules for Environmental Bioremediation Om V. Singh Anuj K. Chandel 141

11 The Application of Molecular Techniques to the Study of Wastewater Treatment Systems Irena Wojnowska-Baryta AgnieszkaCydzik-Kwiatkowska Magdalena Zielińska 157

12 Plasmid-Mediated Bioaugmentation of Wastewater Microbial Communities in a Laboratory-Scale Bioreactor Stephan Bathe Martina Hausner 185

13 The Role of Decision Support for Bioremediation Strategies, Exemplified by Hydrocarbons for In Site and Ex Situ Procedures Elizabeth E. Diplock Dave P. Mardlin Kenneth S. Killham Graeme I. Paton 201

14 Monitored Natural Attenuation Kirsten S. Jørgensen Jani M. Salminen Katarina Björklöf 217

15 Application of Bioassays for the Ecotoxicity Assessment of Contaminated Soils María D. Fernández Mar Babín José V. Tarazona 235

16 Community-Level Physiological Profiling Kela P. Weber Raymond L. Legge 263

Subject Index 283

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