Bioremediation: Methods and Protocols / Edition 1

Bioremediation: Methods and Protocols / Edition 1

by Stephen P. Cummings
     
 

ISBN-10: 1607614383

ISBN-13: 9781607614388

Pub. Date: 11/16/2009

Publisher: Springer-Verlag New York, LLC

Since its first systematic application during the 1970s, bioremediation, or the exploitation of a biological system’s degradative potential to combat toxic pollutants such as heavy metals, polyaromatic hydrocarbons (PAH), cyanides, and radioactive material, has proven itself over time, and the many advances in molecular techniques have only amplified its

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Overview

Since its first systematic application during the 1970s, bioremediation, or the exploitation of a biological system’s degradative potential to combat toxic pollutants such as heavy metals, polyaromatic hydrocarbons (PAH), cyanides, and radioactive material, has proven itself over time, and the many advances in molecular techniques have only amplified its utility. In Bioremediation: Methods and Protocols, experts in the field explore imaginative and ambitious multidisciplinary techniques that will enable more predictable removal of pollutants from a variety of environments. The easy-to-follow volume addresses some of the broader issues such as the effect of the environment in determining the availability and fate of organic and inorganic compounds and how choices around the most appropriate bioremediation process can be arrived at, as well as detailed complementary techniques that support the effective deployment and monitoring of a bioremediation approach. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioremediation: Methods and Protocols offers researchers a series of invaluable techniques certain to enhance their work with environmental contamination.

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Product Details

ISBN-13:
9781607614388
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/16/2009
Series:
Methods in Molecular Biology, #599
Edition description:
2010
Pages:
285
Product dimensions:
7.10(w) x 10.10(h) x 0.90(d)

Table of Contents

1. Measurement of Bioaccessibility of Organic Pollutants in Soil
Laura McAllister and Kirk T. Semple

2. Heavy Metal Bioavailability and Bioaccessibility in Soil
John Richard Dean

3. Addressing PCR Biases in Environmental Microbiology Studies
Rita Sipos, Anna Székely, Sára Révész, and Károly Márialigeti

4. MPN- and Real Time -Based PCR Methods for the Quantification of Alkane-Monooxygenase Homologous Genes (alkB) in Environmental Samples
Alfredo Perez de Mora, Stephan Schulz, and Michael Schloter

5. Application of Fingerprinting Molecular Methods in Bioremediation Studies
Dimitrios G. Karpouzas and Brajesh K. Singh

6. Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Assess Microbial Community Structure in Compost Systems
Sonia M. Tiquia

7. Combination of Fluorescence in situ Hybridization with Staining Techniques for Cell Viability and Accumulation of PHA and polyP in Microorganisms in Complex Microbial Systems
Jeppe Lund Nielsen, Caroline Kragelund, and Per Halkjær Nielsen

8. Combination of Fluorescence in situ Hybridization with Different Extracellular Staining Techniques for Ecophysiological Analysis of Microorganisms in Complex Microbial Systems
Jeppe Lund Nielsen, Caroline Kragelund, and Per Halkjær Nielsen

9. Applications of DNA-Stable Isotope Probing in Bioremediation Studies
Yin Chen, Jyotsna Vohra, and J. Colin Murrell

10. Two-Dimensional Gel Electrophoresis: Discovering Bio-Molecules for Environmental Bioremediation
Om V. Singh and Anuj K. Chandel

11. The Application of Molecular Techniques to the Study of Wastewater Treatment Systems
Irena Wojnowska-Baryla, Agnieszka Cydzik-Kwiatkowska, and Magdalena Zielinska

12. Plasmid-Mediated Bioaugmentation of Wastewater MicrobialCommunities in a Laboratory Scale Bioreactor
Stephan Bathe and Martina Hausner

13. The Role of Decision Support for Bioremediation Strategies, Exemplified by Hydrocarbons for in site and ex situ Procedures
Elizabeth E. Diplock, Dave P. Mardlin, Kenneth S. Killham, and Graeme I. Paton

14. Monitored Natural Attenuation
Kirsten S. Jørgensen, Jani M. Salminen, and Katarina Björklöf

15. Application of Bioassays for the Ecotoxicity Assessment of Contaminated Soils
María D. Fernández, Mar Babin, and José V. Tarazona

16. Community Level Physiological Profiling
Kela P. Weber and Raymond L. Legge

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