Biosensors with Fiberoptics

by Jr. Wingard, Donald L. Wise

Overview

Focusing on the rapidly increasing interaction between biotechnology and advanced fiberoptics/electronics, Biosensors with Fiberoptics emphasizes the three major phases of the developmental process from concept to marketplace: research, development, and applications.

With contributions by leading experts directly involved in key areas of this exciting, fast-paced field, the book describes novel biosensor technologies and such current and potential applications as: chemical sensing • fluorescent labels • evanescent-wave biosensors and immunosensors • clinical applications • immunoassay kinetics • luminescence • monitoring of environmental toxins.

Researchers and clinicians in a broad spectrum of disciplines will find Wise and Wingard's Biosensors with FIBEROPTICS an extremely practical, up-to-date reference text.

Product Details

ISBN-13:	9780896031906
Publisher:	Springer-Verlag New York, LLC
Publication date:	03/01/1991
Series:	Contemporary Instrumentation and Analysis
Edition description:	1991
Pages:	370
Product dimensions:	6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

Concepts, Biological Components, and Scope of Biosensors.- 1. Concept of a Biosensor.- 2. Biological Components.- 3. Transducers.- 4. Potential Applications.- References.- Chemical Sensing with Fiberoptic Devices.- 1. Introduction.- 2. Classification.- 3. Instrumentation.- 4. Analytical Characteristics.- 5. pH Sensors.- 6. Sensors for Anions.- 7. Sensors for Cations.- 8. Sensors for Gases.- 9. Sensors for Biochemicals.- 10. Sensors for Organic Compounds.- 11. Miscellaneous Sensors.- 12. Conclusions.- References.- Fluorescent Labels.- 1. Introduction.- 2. Fluorescence Excitation and Detection.- 3. Chemically Reactive Groups.- 4. Conclusions.- References.- Chemistry and Technology of Evanescent Wave Biosensors.- 1. Introduction.- 2. Why Fiberoptics?.- 3. Fiberoptic Fundamentals.- 4. Configurations for Fiberoptic Biosensors.- 5. Biochemical Architecture of Biosensors.- 6. Approaches for Improved Fiberoptic Biosensors.- 7. Summary.- Acknowledgments.- References.- Optical Characteristics of Fiberoptic Evanescent Wave Sensors Theory and Experiment.- 1. Introduction.- 2. Theory of Evanescent Wave Excitation and Detection of Fluorescence.- 3. Results Calculated from Theory.- 4. Results Based on Experiments.- 5. Summary and Conclusions.- 6. Symbols.- Acknowledgments.- References.- Evanescent Wave Immunosensors for Clinical Diagnostics.- 1. Introduction.- 2. Materials and Methods.- 3. Characterization of the Fiber as a Solid-Phase Surface-Isotopic Model.- 4, Fiberoptic Evanescent Wave Immunoassay (EWS).- 5. Summary and Discussion.- References.- Instrumentation for Cylindrical Waveguide Evanescent Fluorosensors.- 1. Introduction.- 2. Evanescent Waves.- 3. Importance of Numerical Aperture.- 4. Why Cylindrical Waveguides?.- 5. Optical System.- 6. Excitation System.- 7. Detection System.- 8. Filters.- 9. Optical Background.- 10. Sensitivity.- 11. Multiplex Operation.- 12. Applications.- Acknowledgments.- References.- Immunoassay Kinetics at Continuous Surfaces.- 1. Introduction.- 2. Abbreviations.- 3. The Continuous Surface.- 4. Effects of Diffusion at Continuous Surfaces.- 5. Optical Techniques Applied to Immunoassays at a Continuous Surface.- 6. Discussion and Conclusions.- Acknowledgment.- References.- Luminescence in Biosensor Design.- 1. Introduction.- 2. Bioluminescent Reactions.- 3. Immobilized Enzymes for Bioluminescent Analysis 2%.- 5. Bio-and Chemiluminescence Analysis with Fiberoptic Sensors.- 6. Conclusion and Future Trends.- References.- In Vivo Applications of Fiberoptic Chemical Sensors.- 1. Introduction.- 2. Critical Issues and Design Criteria.- 3. Oxygen Sensors.- 4. pH Sensors.- 5. Carbon Dioxide Sensors.- 6. Conclusions.- References.

Editorial Reviews

Focusing on the increasing interaction between biotechnology and advanced fiberoptics/electronics, this volume emphasizes the three major phases of the developmental process: research, development, and applications. It describes biosensor technologies and such current and potential applications as chemical sensing, fluorescent labels, immunoassay kinetics, luminescence, and monitoring of environmental toxins. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

Many of the key issues are well covered in this book which must be recommended as a necessary addition to any biosensor library.-The Genetic Engineer & Biotechnologist

From the Publisher