Bone Research Protocols / Edition 1

Bone Research Protocols / Edition 1

by Miep H. Helfrich
     
 

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ISBN-10: 1588290441

ISBN-13: 9781588290441

Pub. Date: 04/28/2003

Publisher: Springer-Verlag New York, LLC

Laboratory techniques presented here will help researchers investigating the mechanisms by which certain genes affect bone mass and strength. These reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological testing, and the use of digital imaging techniques in the analysis of bone. Although in

Overview

Laboratory techniques presented here will help researchers investigating the mechanisms by which certain genes affect bone mass and strength. These reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological testing, and the use of digital imaging techniques in the analysis of bone. Although in vitro methods receive most attention, there are also protocols for in vivo analyses. The editors are affiliated with the University of Aberdeen Medical School, UK. Annotation ©2003 Book News, Inc., Portland, OR

Product Details

ISBN-13:
9781588290441
Publisher:
Springer-Verlag New York, LLC
Publication date:
04/28/2003
Series:
Methods in Molecular Medicine Series
Edition description:
Older Edition
Pages:
468
Product dimensions:
6.20(w) x 9.20(h) x 1.10(d)

Table of Contents

Part I. Culture of Osteoblasts and Osteocytes

Human Osteoblast Culture
James A. Gallagher

Osteoblast Isolation from Murine Calvariae and Long Bones
Astrid Bakker and Jenneke Klein-Nulend

Mineralizing Fibroblast-Colony-Forming Assays
Andrew Scutt, Lynsey Reading, Nanette Scutt, and Karen Still

Osteocyte Isolation and Culture
Peter J. Nijweide, Arie van der Plas, Marcel J. Alblas, and Jenneke Klein-Nulend

Part II. Culture of Osteoclasts. Isolated Osteoclast Cultures
Astrid Hoebertz and Timothy R. Arnett

Primary Isolation and Culture of Chicken Osteoclasts
Patricia Collin-Osdoby, Fred Anderson, and Philip Osdoby

Isolation and Purification of Rabbit Osteoclasts
Fraser P. Coxon, Julie C. Frith, Helena L. Benford, and Michael J. Rogers

Generating Human Osteoclasts from Peripheral Blood
Afsie Sabokbar and Nicholas A. Athanasou

Generating Human Osteoclasts from Bone Marrow and Peripheral Blood
Adrienne M. Flanagan and Helen M. Massey

Generating Murine Osteoclasts from Bone Marrow
Naoyuki Takahashi, Nobuyuki Udagawa, Sakae Tanaka, and Tatsuo Suda

Osteoclast Formation in the Mouse Coculture Assay
Robert J. van 't Hof

RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells
Patricia Collin-Osdoby, Xuefeng Yu, Hong Zheng, and Philip Osdoby

Part III. Bone Organ Culture

Analysis of Osteoclast Function in Mouse Calvarial Cultures
Michael J. Marshall and Marit N. Rowlands

Assessing Bone Formation Using Mouse Calvarial Organ Culture
I. Ross Garrett

Part IV. Histological and Ultrastructural Techniques

In Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone Sections
Andrew P. Mee and Judith A. Hoyland

Techniques for the Study of Apoptosis in Bone
Brendon S. Noble and Hazel Y. Stevens

Protein Localization in Wax-Embedded and Frozen Sections of Bone Using Immunohishemistry
Sharyn Bord

Detection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone Sections
Johannes P. T. M. van Leeuwen and Pieter Derkx

Fluorescence Imaging of Bone-Resorbing Osteoclasts by Confocal Microscopy
Stephen A. Nesbitt and Michael A. Horton

Bone Histomorphometry
Shobna Vedi and Juliet Compston

Transmission Electron Microscopy of Bone
Vincent Everts, Anneke Niehof, and Wouter Beertsen

Scanning Electron Microscopy of Bone
Deborah Marshall, Miep H. Helfrich, and Richard M. Aspden

Part V. Imaging Techniques

Bone Measurement by Peripheral Quantitative Computed Tomography in Rodents
Jürg A. Gasser

Part VI. In Vivo Techniques. Studies of Local Bone Remodeling: The Calvarial Injection Assay
Robert J. van 't Hof, Claire E. Clarkin, and Kenneth J. Armour

Inflammation-Induced Osteoporosis: The IMO Model
Kenneth J. Armour and Katharine E. Armour

Ovariectomy and Estrogen Replacement in Rodents
Jade W. M. Chow

Part VII. Mechanical Loading Techniques

Mechanical Testing of Bone Ex Vivo
Richard M. Aspden

Bone Cell Responses to Fluid Flow
Hazel Y. Stevens and John A. Frangos

Methods for Analyzing Bone Cell Responses to Mechanical Loading Using In Vitro Monolayer and Organ Culture Models
Andrew A. Pitsillides, Victoria Das-Gupta, Dominic Simon, and Simon C. F. Rawlinson

Part VIII. Molecular Analysis

Extraction of Nucleic Acids from Bone
Tracy L. Stewart and Val Mann

Analysis of Gene Expression in Bone by Quantitative RT-PCR
Deborah Ireland

Index

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