Bone Research Protocols / Edition 1

Hardcover (Print)
Buy Used
Buy Used from
(Save 40%)
Item is in good condition but packaging may have signs of shelf wear/aging or torn packaging.
Condition: Used – Good details
Used and New from Other Sellers
Used and New from Other Sellers
from $28.00
Usually ships in 1-2 business days
(Save 82%)
Other sellers (Hardcover)
  • All (9) from $28.00   
  • New (3) from $90.27   
  • Used (6) from $28.00   
Sort by
Page 1 of 1
Showing All
Note: Marketplace items are not eligible for any coupons and promotions
Seller since 2008

Feedback rating:



New — never opened or used in original packaging.

Like New — packaging may have been opened. A "Like New" item is suitable to give as a gift.

Very Good — may have minor signs of wear on packaging but item works perfectly and has no damage.

Good — item is in good condition but packaging may have signs of shelf wear/aging or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Acceptable — item is in working order but may show signs of wear such as scratches or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Used — An item that has been opened and may show signs of wear. All specific defects should be noted in the Comments section associated with each item.

Refurbished — A used item that has been renewed or updated and verified to be in proper working condition. Not necessarily completed by the original manufacturer.


Ships from: Chicago, IL

Usually ships in 1-2 business days

  • Standard, 48 States
  • Standard (AK, HI)
Seller since 2015

Feedback rating:


Condition: New
Brand new.

Ships from: acton, MA

Usually ships in 1-2 business days

  • Standard, 48 States
  • Standard (AK, HI)
Seller since 2015

Feedback rating:


Condition: New
Brand new.

Ships from: acton, MA

Usually ships in 1-2 business days

  • Standard, 48 States
  • Standard (AK, HI)
Page 1 of 1
Showing All
Sort by


Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories.

Read More Show Less

Editorial Reviews

From the Publisher
"Bone Research Prools is a most welcome compilation of methods. In it, many of the most productive laboratories in some of the most challenging areas of cell biology reveal detailed prools that have been used to solve key problems. Short of spending a sabbatical in one of the eminent laboratories that contributed, this volume provides the best possible introduction to these methods." - TRENDS in Endocrinology and Metabolism
Read More Show Less

Product Details

  • ISBN-13: 9781588290441
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 4/28/2003
  • Series: Methods in Molecular Medicine Series
  • Edition description: Older Edition
  • Edition number: 1
  • Pages: 468
  • Product dimensions: 6.20 (w) x 9.20 (h) x 1.10 (d)

Table of Contents

1 Human Osteoblast Culture 3
2 Osteoblast Isolation from Murine Calvariae and Long Bones 19
3 Mineralizing Fibroblast-Colony-Forming Assays 29
4 Osteocyte Isolation and Culture 41
5 Isolated Osteoclast Cultures 53
6 Primary Isolation and Culture of Chicken Osteoclasts 65
7 Isolation and Purification of Rabbit Osteoclasts 89
8 Generating Human Osteoclasts from Peripheral Blood 101
9 Generating Human Osteoclasts from Bone Marrow and Peripheral Blood 113
10 Generating Murine Osteoclasts from Bone Marrow 129
11 Osteoclast Formation in the Mouse Coculture Assay 145
12 RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells 153
13 Analysis of Osteoclast Function in Mouse Calvarial Cultures 169
14 Assessing Bone Formation Using Mouse Calvarial Organ Culture 183
15 In Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone Sections 201
16 Techniques for the Study of Apoptosis in Bone 225
17 Protein Localization in Wax-Embedded and Frozen Sections of Bone Using Immunohistochemistry 237
18 Detection of Noncollagenous Bone Proteins in Methylmetacrylate-Embedded Human Bone Sections 249
19 Fluorescence Imaging of Bone Resorbing Osteoclasts by Confocal Microscopy 259
20 Bone Histomorphometry 283
21 Transmission Electron Microscopy of Bone 299
22 Scanning Electron Microscopy of Bone 311
23 Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents 323
24 Studies of Local Bone Remodeling: The Calvarial Injection Assay 345
25 Inflammation-Induced Osteoporosis: The IMO Model 353
26 Ovaridectomy and Estrogen Replacement in Rodents 361
27 Mechanical Testing of Bone Ex Vivo 369
28 Bone Cell Responses to Fluid Flow 381
29 Methods for Analyzing Bone Cell Responses to Mechanical Loading Using In Vitro Monolayer and Organ Culture Models 399
30 Extraction of Nucleic Acids from Bone 425
31 Analysis of Gene Expression in Bone by Quantitative RT-PCR 433
Index 441
Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)