Microbes as Tools for Cell Biology / Edition 1

Microbes as Tools for Cell Biology / Edition 1

by Leslie Wilson, Leslie Wilson
     
 

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ISBN-10: 012564146X

ISBN-13: 9780125641463

Pub. Date: 01/04/1995

Publisher: Elsevier Science

Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of microbes, assays for

Overview

Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of microbes, assays for pathogen-host recognition, and analysis of intracellular parasitism. Each chapter outlines practical procedures and describes the rationale behind their development. This volume should prove useful to anyone interested in the biology of infectious agents, or their exploitation as a new generation of cell biological reagents.

Key Features
• Introduction by renowned microbiologist Dr. Stanley Falkow
• Covers manipulation of pathogens, especially generation and selection of non-virulent phenotypes
• Guides researchers in the study of intracellular pathogenesis
• Describes microbial adherence and phagocytosis assays
• Focuses on protein trafficking in infected cells
• Well-illustrated with color plates, halftones, and diagrams

Product Details

ISBN-13:
9780125641463
Publisher:
Elsevier Science
Publication date:
01/04/1995
Series:
Methods in Cell Biology Series, #45
Pages:
339
Product dimensions:
7.52(w) x 9.25(h) x (d)

Table of Contents

D.G. Russell, Preface.
S. Falkow, Introduction.
D.G. Russell, Obtaining and Maintaining Microbial Pathogens.
Manipulation of Pathogens:
W. Trager, Cultivation of Malaria Parasites.
D.S. Roos, R.G.K. Donald, N.S. Morrissette, and A.L.C. Moulton, Molecular Tools for Genetic Dissection of the Protozoan Parasite Toxoplasma gondii.
J.H. LeBowitz, Transfection Experiments with Leishmania.
R. Tsolis and F. Heffron, Mutagenesis and Variant Selection in Salmonella.
N.D. Connell, Mycobacterium: Isolation, Maintenance, Transformation, and Mutant Selection.
Microbial Adherence and Invasion Assays:
G.J. Bancroft, H.L. Collins, L.B. Sigola, and C.E. Cross, Modulation of Murine Macrophage Behavior in Vivo and in Vitro.
E.J. Brown, In Vitro Assays of Phagocytic Function of Human Peripheral Blood Leukocytes: Receptor Modulation and Signal Transduction.
P. Falk, T. Borén, D. Haslam, and M. Caparon, Bacterial Adhesion and Colonization Assays.
I. Crandall and I.W. Sherman, Cytoadherence and the Plasmodium falciparum-Infected Erythrocyte.
The Study of Intracellular Pathogenesis:
M.J. Blackman, Purification of Plasmodium falciparum Merozoites for Analysis of the Processing of Merozoite Surface Protein-1.
K. Haldar, H.G. Elmendorf, A. Das, W.L. Li, D.J.P. Ferguson, and B.C. Elford, In Vitro Secretory Assays with Erythrocyte-Free Malaria Parasites.
K.H. Berger and R.R. Isberg, Intracellular Survival by Legionella.
P. Chakraborty, S. Sturgill-Koszycki, and D.G. Russell, Isolation and Characterization of Pathogen-Containing Phagosomes.
D.G. Russell, Immunoelectron Microscopy of Endosomal Trafficking in Macrophages Infected with Microbial Pathogens.
P.H. Schlesinger, Measuring the pH of Pathogen-Containing Phagosomes.
C.V. Harding, Techniques for Studying Phagocytic Processing of Bacteria for Class I or II MHC-Restricted Antigen Recognition by T Lymphocytes.
Chapter References.
Subject Index.
Volumes in Series.

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