Molecular Biology Problem Solver: A Laboratory Guide / Edition 1

Molecular Biology Problem Solver: A Laboratory Guide / Edition 1

by Alan S. Gerstein
     
 

ISBN-10: 0471379727

ISBN-13: 2900471379729

Pub. Date: 10/08/2001

Publisher: Wiley

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. A unique question-based format reviews common assumptions and…  See more details below

Overview

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Alan Gerstein and his collaborators have been guiding researchers of all levels of expertise through the troubleshooting process over the course of three decades, assuring that Molecular Biology Problem Solver will prove an excellent resource for all life scientists.

Product Details

ISBN-13:
2900471379729
Publisher:
Wiley
Publication date:
10/08/2001
Edition description:
New Edition
Pages:
596

Table of Contents

Preface
Contributors
Ch. 1Preparing for Success in the Laboratory1
Ch. 2Getting What You Need from a Supplier11
Ch. 3The Preparation of Buffers and Other Solutions: A Chemist's Perspective31
Ch. 4How to Properly Use and Maintain Laboratory Equipment49
Ch. 5Working Safely with Biological Samples113
Ch. 6Working Safely with Radioactive Materials141
Ch. 7DNA Purification167
Ch. 8RNA Purification197
Ch. 9Restriction Endonucleases225
Ch. 10Nucleotides, Oligonucleotides, and Polynucleotides267
Ch. 11PCR291
Ch. 12Electrophoresis331
Ch. 13Western Blotting373
Ch. 14Nucleic Acid Hybridization399
Ch. 15E. coli Expression Systems461
Ch. 16Eukaryotic Expression491
Index543

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