Cancer Genomics and Proteomics: Methods and Protocols

by Paul B. Fisher (Editor)

Overview

Cancer Genomics and Proteomics provides a compendium of techniques and applications in gene identification and function. The approaches described in detail are state-of-the art and can be tailored to individual ongoing or planned research projects. This volume is a valuable laboratory resource for designing experiments to identify and analyze genes that are relevant to complex biological phenomena.

Product Details

ISBN-13:	9781617376054
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/19/2010
Series:	Methods in Molecular Biology , #383
Edition description:	Softcover reprint of hardcover 1st ed. 2007
Pages:	358
Product dimensions:	5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

Reciprocal Subtraction Differential RNA Display (RSDD).- Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH).- The Application of Differential Display as a Gene Profiling Tool.- Serial Analysis of Gene Expression (SAGE).- Gene Expression Profile Analysis of Tumors.- cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-κB Related Genes in Squamous Cell Carcinoma.- Gene Profiling Uncovers Retinoid Target Genes.- Complete Open Reading Frame (C-ORF) Technique.- Chromatin Immunoprecipitation Assays.- Manipulating Genes and Gene Copy Number by Bacterial Artificial Chromosome Transfection.- Molecular Cytogenetic Applications in Analysis of the Cancer Genome.- Monitoring Methylation and Gene Expression in Cancer.- The Use of Phage Display Peptide Libraries for Basic and Translational Research.- Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions.- Ribonomic and Short Hairpin RNA Gene Silencing Methods to Explore Functional Gene Programs Associated With Tumor Growth Arrest.- Surface-Epitope Masking (SEM).- Approaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells.- PKR in Innate Immunity, Cancer, and Viral Oncolysis.- Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells.- Analysis of Growth Properties and Cell Cycle Regulation Using Mouse Embryonic Fibroblast Cells.- Reverse Phase Protein Microarrays for Monitoring Biological Responses.- Protein Crystallization.

Editorial Reviews

From the reviews:

"A compendium of techniques and applications that will be of wide utility to researchers interested in gene identification and function. … this volume will be of interest to a broad audience of molecular biologists, geneticists and biochemists and will provide a valuable resource in the laboratory due to its applicability to a wide breadth of projects. … for labs with a strong interest in proteomics and genomics it would certainly be well worth buying a copy to share. … I would certainly recommend this book." (Barbara Guinn, Immunology News, May, 2008)

From the Publisher