Cancer Genomics and Proteomics: Methods and Protocols / Edition 1

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Cancer Genomics and Proteomics provides a compendium of techniques and applications in gene identification and function. The approaches described in detail are state-of-the-art and can be tailored to individual ongoing or planned research projects. This volume is a valuable laboratory resourcefor designing experiments to identify and analyze genes that are relevant to complex biological phenomena.

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Editorial Reviews

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"A compendium of techniques and applications that will be of wide utility to researchers interested in gene identification and function. … this volume will be of interest to a broad audience of molecular biologists, geneticists and biochemists and will provide a valuable resource in the laboratory due to its applicability to a wide breadth of projects. … for labs with a strong interest in proteomics and genomics it would certainly be well worth buying a copy to share. … I would certainly recommend this book." (Barbara Guinn, Immunology News, May, 2008)

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Product Details

  • ISBN-13: 9781617376054
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/19/2010
  • Series: Methods in Molecular Biology Series, #383
  • Edition description: Softcover reprint of hardcover 1st ed. 2007
  • Edition number: 1
  • Pages: 358
  • Product dimensions: 0.84 (w) x 6.00 (h) x 9.00 (d)

Table of Contents

Table of Contents

Cancer Genomics and Proteomics: Methods and Prools, Paul B. Fisher, Ed.

1. Devanand Sarkar, Dong-chul Kang and Paul B. Fisher
Reciprocal Subtraction Differential RNA Display (RSDD): An Efficient Technology for Cloning Differentially Expressed Genes

2. Habib Boukerche, Zao-zhong Su, Dong-chul Kang & Paul B. Fisher
Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH)

3. Ken C.N. Chang, Barry Komm, Nichole Boyer Arnold, and Murray Korc
The Application of Differential Display as a Gene Profiling Tool

4. Fred van Ruissen and Frank Baas
Serial Analysis of Gene Expression (SAGE)

5. Katia Basso, and Riccardo Dalla-Favera
Gene expression profile analysis of tumors

6. Zhong Chen, Tin-Lap Lee, Xin-Ping Yang, Gang Dong, Amy Loercher, and Carter Van Waes cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-KappaB Related Genes in Squamous Cell Carcinoma

7. Yan Ma, Qing Feng, Ian Pitha-Rowe, Sutisak Kitareewan and
Ethan Dmitrovsky
Gene Profiling Uncovers Retinoid Target Genes

8. Dong-chul Kang and Paul B. Fisher
Complete Open Reading Frame (C-ORF) Technique: A Rapid and Efficient Method for Obtaining Complete Protein Coding Sequences

9. Piyali Dasgupta and Srikumar P. Chellappan
Chromatin Immunoprecipitation Assays: Molecular Analysis of Chromatin Modification and Gene Regulation

10. Stephanie F. Phelps, Sharon Illenye and Nicholas H. Heintz
Manipulating Genes and Gene Copy Number by BAC Transfection

11. Pulivarthi H. Rao, Subhadra V. Nandula, and Vundavalli V. Murty
Molecular Cytogenetic Applications in the Analysis of Cancer Genome

12. Hetty Carraway and James Herman
Evaluation of DNA Methylation

13. Renee Brissette and Neil I. Goldstein
The Use of Phage Display Peptide Libraries for Basicand Translational Research

14. Shu-ichi Matzsuzawa and John C. Reed
Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions

15. Timothy E. Baroni, Michele T. Lastro, Aparna C. Ranganathan, Scott A.
Tenenbaum, Douglas S. Conkin and Julio Aguirre-Ghiso
Ribonomic and Short Hairpin RNA Gene Silencing Methods to Explore Functional Gene Programs Associated with Tumor Growth Arrest

16. Neil I. Goldstein and Paul B. Fisher
Surface-Epitope Masking (SEM): An Immunological Subtraction Approach for Developing Monoclonal Antibodies Targeting Surface-Expressed Molecules

17. Paul Dent, Philip B. Hylemon, Steven Grant and Paul B. Fisher
Approaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells

18. Siddharth Balachandran and Glen N. Barber
PKR in Innate Immunity, Cancer, and Viral Oncolysis

19. Hong Sun and Reshma Taneja
Analysis of Transformation and Tumorigenicity using Mouse Embryonic Fibroblast Cells

20. Hong Sun, Neriman Tuba Gulbagci and Reshma Taneja-
Analysis of Growth Properties and Cell Cycle Regulation using Mouse Embryonic Fibroblast Cells

21. Virginia Espina, Julia Wulfkuhle, Emanuel F. Petricoin III & Lance A. Liotta
Reverse Phase Protein Microarrays for Monitoring Biologic Responses

22. Champion Deivanayagam, William J. Cook and Mark R. Walter
Protein Crystallization

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