Capillary Electrophoresis: Principles, Practice and Applications / Edition 3

Capillary Electrophoresis: Principles, Practice and Applications / Edition 3

by S.F.Y. Li, Po Li
     
 

Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE… See more details below

Overview

Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals and environmental pollutants have been demonstrated. A wide range of applications have been developed in greatly diverse fields, such as chemical, biotechnological, environmental and pharmaceutical analysis.

This book covers all aspects of CE, from the principles and technical aspects to the most important applications. It is intended to meet the growing need for a thorough and balanced treatment of CE. The book will serve as a comprehensive reference work. Both the experienced analyst and the newcomer will find the text useful.

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Product Details

ISBN-13:
9780444815903
Publisher:
Elsevier Science
Publication date:
06/30/1993
Series:
Journal of Chromatography Library Series
Pages:
581
Product dimensions:
1.27(w) x 6.14(h) x 9.21(d)

Table of Contents

Preface
List of Symbols
Abbreviations
Ch. 1Introduction1
1.1Historical background1
1.2Different modes of capillary electrophoresis4
1.3Principles of separation in capillary zone electrophoresis (CZE)12
1.4Comparison with other separation techniques22
Ch. 2Sample Injection Methods31
2.2Electrokinetic injection33
2.3Hydrodynamic injection37
2.4Electric sample splitter42
2.5Split flow syringe injection system43
2.6Rotary-type injector45
2.7Freeze plug injection45
2.8Sampling device with feeder47
2.9Microinjectors48
2.10Optical gating49
2.11On-column fracture for sample introduction50
Ch. 3Detection Techniques55
3.2UV-visible absorbance detectors56
3.3Photodiode array and multiwavelength UV detection71
3.4Fluorescence detection73
3.5Laser-based thermo-optical and refractive index detectors96
3.6Electrochemical detection105
3.7Indirect detection121
3.8Radioisotope detectors126
3.9Mass spectrometric detection131
Ch. 4Column Technology155
4.2Coated columns158
4.3Columns for capillary gel electrophoresis (CGE)173
4.4Packed columns194
4.5Capillary electrophoresis on a chip195
Ch. 5Electrolyte Systems201
5.2Micellar electrokinetic chromatography (MEKC)232
5.3Use of inclusion complexes259
5.4Complexing additives271
5.5Other types of electrophoretic media283
Ch. 6Special Systems and Methods295
6.2Buffer programming296
6.3Fraction collection305
6.4Field effect electroosmosis311
6.5Systematic optimization schemes316
6.6Determination of electrophoretic mobilities and diffusion coefficients335
6.7Capillary isoelectric focusing (CIEF)341
6.8Capillary isotachophoresis347
6.9Hyphenated techniques355
Ch. 7Applications377
7.1Amino acids378
7.2Peptides383
7.3Proteins397
7.4Nucleic acids419
7.5Pharmaceuticals and drugs438
7.6Cells, viruses and bacteria456
7.7Analysis of body fluids456
7.8Capillary ion analysis462
7.9Metal chelates469
7.10Organic compounds472
7.11Carbohydrates480
7.12Food analysis485
7.13Environmental analysis489
7.14Polymer and particle analysis491
7.15Natural products496
7.16Chiral separation498
7.17Separation of geometrical and positional isomers504
7.18Coal and fuels506
7.19Textile and dyes507
7.20Explosives508
7.21Survey of commercial CE instruments508
7.22Summary of applications of CE512
Ch. 8Recent Advances and Prospect for Growth541
8.1Recent reviews on CE541
8.2Advances in injection techniques541
8.3Novel detection techniques542
8.4Advances in column technology544
8.5Progress on electrolyte systems545
8.6New systems and methods546
8.7Additional applications based on CE547
8.8Future trends550
Subject index555

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