Capillary Electrophoresis: Principles, Practice and Applications / Edition 3by S.F.Y. Li, Po Li
Pub. Date: 06/30/1993
Publisher: Elsevier Science
Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on
Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals and environmental pollutants have been demonstrated. A wide range of applications have been developed in greatly diverse fields, such as chemical, biotechnological, environmental and pharmaceutical analysis.
This book covers all aspects of CE, from the principles and technical aspects to the most important applications. It is intended to meet the growing need for a thorough and balanced treatment of CE. The book will serve as a comprehensive reference work. Both the experienced analyst and the newcomer will find the text useful.
Table of Contents1. Introduction. Historical Background. Overview of High Performance CE. Principles of Separations. Comparison with Other Separation Techniques. 2. Sample Injection Methods. Introduction. Electrokinetic Injection. Hydrodynamic Injection. Electric Sample Splitter. Split Flow Syringe Injection System. Rotary Type Injector. Freeze Plug Injection. Sampling Device with Feeder. Microinjectors. Optical Gating. 3. Detection Techniques. Introduction. UV-Visible Absorbance Detectors. Photo-diode Array Detectors. Fluorescence Detectors. Laser-based Thermooptical and Refractive Index Detectors. Indirect Detection. Conductivity Detection. Electrochemical Detection. Mass Spectrometric Detection. 4. Column Technology. Uncoated Capillary Columns. Coated Columns. Gel-filled Columns. Packed Columns. Combining Packed and Open-Tubular Column. 5. Electrophoretic Media. Electrophoretic Buffer Systems. Micellar Electrokinetic Capillary Chromatography. Inclusion Pseudophases. Metal-complexing Pseudophases. Other Types of Electrophoretic Media. 6. Special Systems and Methods. Buffer Programming. Fraction Collection. Hyphenated Techniques. Field Effect Electroosmosis. Systematic Optimization of Separation. 7. Applications of Capillary Electrophoresis. Biomolecules. Pharmaceutical and Clinical Analysis. Inorganic Ions. Hydrocarbons. Foods and Drinks. Environmental Pollutants. Carbohydrates. Toxins. Polymers and Particles. Natural Products. Fuel. Metal Chelates. Industrial Waste Water. Explosives. Miscellaneous Applications. 8. Recent Advances and Prospect for Growth. Recent Reviews on CE. Advances in Injection Techniques. Novel Detection Techniques. Advances inColumn Technology. Progress in Electrolyte Systems. New Systems and Methods. Additional Applications Based on CE. Future Trends. Subject index.
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