Capillary Electrophoresis: Principles, Practice and Applications / Edition 3

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Overview

Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals and environmental pollutants have been demonstrated. A wide range of applications have been developed in greatly diverse fields, such as chemical, biotechnological, environmental and pharmaceutical analysis.

This book covers all aspects of CE, from the principles and technical aspects to the most important applications. It is intended to meet the growing need for a thorough and balanced treatment of CE. The book will serve as a comprehensive reference work. Both the experienced analyst and the newcomer will find the text useful.

Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals and environmental pollutants have been demonstrated. A wide range of applications have been developed in greatly diverse fields, such as chemical, biotechnological, environmental and pharmaceutical analysis.

This book covers all aspects of CE, from the principles and technical aspects to the most important applications. It is intended to meet the growing need for a thorough and balanced treatment of CE. The book will serve as a comprehensive reference work. Both the experienced analyst and the newcomer will find the text useful

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Editorial Reviews

From the Publisher
...anybody wanting to write a book on CE after this would look like a fool. Everything seems to be there, any detection system you have ever dreamed of, any capillary coating, enough electrolyte systems to saturate your wits, and more, and more.

...by far the most thorough book in the field yet to appear.
Journal of Chromatography
Anyone reading this book cannot fail to agree with Li's conclusion that the potential of the technique is tremendous.
Analytical Instrument Industry Report
...a prime source of information both for newcomers and for those versed in the technique. It is strongly recommended.
Endeavour
...a very useful addition to the literature of separation science encompassing, as it does, all aspects of this rapidly emerging family of methods. It is almost encyclopaedic in its coverage and all chapters are extremely well referenced. This will facilitate any search for original information on any aspect of CE.
Talanta
All you ever wanted to know about CE can be found in this excellent book. It is a must for beginners and a valuable reference book for the experienced.
Journal of Applied Toxicology
...a quite useful reference and is highly recommended for libraries and laboratories applying this ever-growing technique, both in industry and academic institutions.
Journal of Liquid Chromatography
...an invaluable guide to both novice and experienced researchers in the field.
International Journal of Food Science & Technology
This is a useful book that serves several audiences. The author is to be commended for putting together a thoughtful, complete, and thoroughly useful reference.
Analytical Chemistry
The author is to congratulated on achieving the objectives for the book. It can be recommended to anyone who wishes to get grips with a techniques that can generate up to thirty million theoretical plates per metre: that should include all separation scientists.
The Analyst

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Product Details

  • ISBN-13: 9780444815903
  • Publisher: Elsevier Science
  • Publication date: 6/30/1993
  • Series: Journal of Chromatography Library Series
  • Edition number: 3
  • Pages: 581
  • Product dimensions: 1.27 (w) x 6.14 (h) x 9.21 (d)

Table of Contents

Preface
List of Symbols
Abbreviations
Ch. 1 Introduction 1
1.1 Historical background 1
1.2 Different modes of capillary electrophoresis 4
1.3 Principles of separation in capillary zone electrophoresis (CZE) 12
1.4 Comparison with other separation techniques 22
Ch. 2 Sample Injection Methods 31
2.2 Electrokinetic injection 33
2.3 Hydrodynamic injection 37
2.4 Electric sample splitter 42
2.5 Split flow syringe injection system 43
2.6 Rotary-type injector 45
2.7 Freeze plug injection 45
2.8 Sampling device with feeder 47
2.9 Microinjectors 48
2.10 Optical gating 49
2.11 On-column fracture for sample introduction 50
Ch. 3 Detection Techniques 55
3.2 UV-visible absorbance detectors 56
3.3 Photodiode array and multiwavelength UV detection 71
3.4 Fluorescence detection 73
3.5 Laser-based thermo-optical and refractive index detectors 96
3.6 Electrochemical detection 105
3.7 Indirect detection 121
3.8 Radioisotope detectors 126
3.9 Mass spectrometric detection 131
Ch. 4 Column Technology 155
4.2 Coated columns 158
4.3 Columns for capillary gel electrophoresis (CGE) 173
4.4 Packed columns 194
4.5 Capillary electrophoresis on a chip 195
Ch. 5 Electrolyte Systems 201
5.2 Micellar electrokinetic chromatography (MEKC) 232
5.3 Use of inclusion complexes 259
5.4 Complexing additives 271
5.5 Other types of electrophoretic media 283
Ch. 6 Special Systems and Methods 295
6.2 Buffer programming 296
6.3 Fraction collection 305
6.4 Field effect electroosmosis 311
6.5 Systematic optimization schemes 316
6.6 Determination of electrophoretic mobilities and diffusion coefficients 335
6.7 Capillary isoelectric focusing (CIEF) 341
6.8 Capillary isotachophoresis 347
6.9 Hyphenated techniques 355
Ch. 7 Applications 377
7.1 Amino acids 378
7.2 Peptides 383
7.3 Proteins 397
7.4 Nucleic acids 419
7.5 Pharmaceuticals and drugs 438
7.6 Cells, viruses and bacteria 456
7.7 Analysis of body fluids 456
7.8 Capillary ion analysis 462
7.9 Metal chelates 469
7.10 Organic compounds 472
7.11 Carbohydrates 480
7.12 Food analysis 485
7.13 Environmental analysis 489
7.14 Polymer and particle analysis 491
7.15 Natural products 496
7.16 Chiral separation 498
7.17 Separation of geometrical and positional isomers 504
7.18 Coal and fuels 506
7.19 Textile and dyes 507
7.20 Explosives 508
7.21 Survey of commercial CE instruments 508
7.22 Summary of applications of CE 512
Ch. 8 Recent Advances and Prospect for Growth 541
8.1 Recent reviews on CE 541
8.2 Advances in injection techniques 541
8.3 Novel detection techniques 542
8.4 Advances in column technology 544
8.5 Progress on electrolyte systems 545
8.6 New systems and methods 546
8.7 Additional applications based on CE 547
8.8 Future trends 550
Subject index 555
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