Carbohydrate Biotechnology Protocols / Edition 1

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A compilation of modern hands-on methods using microbes and enzymes to produce and modify carbohydrates of potential and actual commercial value. These powerful methods enable both the expert and the beginner to generate polysaccharides, oligosaccharides, and carbohydrate-based surfactants by fermentation using enzymes. Additional techniques make it possible to produce derivatives of sugars, other oligosaccharides, and sugar derivatives using enzyme technology. Carbohydrate Biotechnology Prools offers synthetic chemists, biochemists, fermentation technologists, and applied enzymologists cutting-edge techniques-many of them hitherto unavailable-that are cleaner and often less costly than available chemical alternatives.

"...presents reproducible techniques for the study of carbohydrates and their application in industrial biotechnological products...includes helpful Notes sections with hints, tricks of the trade, & troubleshooting advice."

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Editorial Reviews

From the Publisher
"...The recommendable book is very useful for students to start experiment work and for scientists and practitioners in doing experimental tasks."-Nahrung

"...the individual chapters are well written by subject matter authorities, and provide useful insights to the original research literature. The bench manual format of the book perhaps makes it most useful to active researchers interested in the specific prools selected for this volume, although a wealth of interesting information is found among the chapter introductions and notes."-ASM News

"Each chapter of the volume is armed with necessary literature references, as basic sources of knowledge. The contributions throughout the book are easily readable and understandable, this is why I recommend the book not only to experts in synthetic chemistry, biochemistry, fermentation biotechnology, and applied enzymology, but also to beginners and students who are new to the field of carbohydrate biotechnology.-Models of Chemistry

"The prools are clearly arranged and begin with a short theoretical and commercial background of the method, the list of reagents and equipment and continue with a comprehensive step-by-step description and a set of notes. Such procedure saves time of the user, indicating quickly which problems are likely to arise. The recommendable book is very useful for students to start experimental work and for scientists and practitioners in doing experimental tasks."-Nahrung

"...a valuable resource to more-experienced practitioners who require alternative methodologies....this volume will prove a useful resource for researchers of graduate level and above, with an interest in investigating carbohydrate biochemistry as applied to the fields of biotechnology and pharmacology." - Tibtech

This compilation of hands-on methods to produce and modify carbohydrates enables both the beginner and the expert to generate a variety of saccharides and carbohydrate-based surfactants by fermentation using enzymes. The manual includes detailed descriptions and methods for using and producing carbohydrate materials; novel methods for producing and modifying low molecular weight carbohydrates; and new tools to elucidate how carbohydrates facilitate inter- and intracellular communication. Intended for synthetic chemists, biochemists, fermentation biotechnologists, and applied enzymologists interested in cutting-edge techniques (many hitherto unavailable in print) that are cleaner and often less costly than available chemical alternatives. Annotation c. Book News, Inc., Portland, OR (
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Product Details

  • ISBN-13: 9781617370748
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/19/2010
  • Series: Methods in Biotechnology Series , #10
  • Edition description: Softcover reprint of hardcover 1st ed. 1999
  • Edition number: 1
  • Pages: 337
  • Product dimensions: 0.79 (w) x 6.00 (h) x 9.00 (d)

Table of Contents

Introduction to Carbohydrate Biotechnology, Christopher Bucke. Production and Isolation of Xanthan Gum, Félix García-Ochoa, Victoria E. Santos, and José A. Casas. Alginate from Azotobacter vinelandii, Francesca Clementi, Mauro Moresi, and Eugenio Parente. Production of Schizophyllan, Udo Rau. Enzymatic Synthesis of Cellulose, Shiro Kobayashi, Shin-ichiro Shoda, Michael J. Donnelly, and Stephen P. Church. Modification of Alginate Using Mannuronan C-5-Epimerases, Helga Ertesvåg and Gudmund Skjåk-Bræk. Viscosity Control of Guar Polysaccharide Solutions by Treatment with Galactose Oxidase and Catalase Enzymes, Michael J. Donnelly. The Production of Cyclodextrins Using CGTase from Bacillus macerans, Jacob A. Rendleman, Jr. Production of Microbial Glycolipids, Siegmund Lang. Partial Enzymatic Hydrolysis of Starch to Maltodextrins on the Laboratory Scale, Leon M. Marchal. The Production of a(1Æ2)-Terminated Glucooligosaccharides, Marguerite Dols, Vincent Monchois, Magali Remaud-Siméon, René-Marc Willemot, and Pierre F. Monsan. Enzymatic Production of Fructooligosaccharides from Sucrose, Jong Won Yun and Seung Koo Song. Enzymatic Production of Inulooligosaccharides from Inulin, Jon Won Yun and Dong Hyun Kim. One-Pot Enzymatic Synthesis of Sialyl T-Epitope, Vladimír Kren. Hydrolysis of Hemicelluloses Using Combinations of Xylanases and Feruloyl Esterases, Craig B. Faulds, Paul A. Kroon, Begoña Bartolomé, and Gary Williamson. Enzymatic Depolymerization of Chitins and Chitosans, Riccardo A. A. Muzzarelli, Vesna Stanic, and Viviana Ramos. Synthesis of Homo- and Hetero-Oligosaccharides from Underivatized Sugars Using Glycosidases, Christopher Bucke, John Packwood, Sony Suwasono, and Robert A. Rastall. Use of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE®) in the Elucidation of N-Linked Oligosaccharide Structures, Harish P. M. Kumar, Patricia A. Denny, and Paul C. Denny. Application of Sucrose Synthase in the Synthesis of Nucleotide Sugars and Saccharides,Astrid Zervosen and Lothar Elling. Production of Isomaltulose Using Immobilized Bacterial Cells, Peter S. J. Cheetham and Christopher Bucke. The Production of Mannitol by Fermentation, Wim Soetaert, Petra T. Vanhooren, and Erick J. Vandamme. The Production of 3-Keto-Derivatives of Disaccharides, Eberhard Stoppok and Klaus Buchholz. Enzymatic Synthesis of a-Butylglucoside in a Biphasic Butanol-Water System Using the a-Transglucosidase from Aspergillus niger, Marie-Pierre Bousquet, René-Marc Willemot, Pierre F. Monsan, François Paul, and Emmanuel Boures. Enzymatic Glycosylation of Aglycones of Pharmacological Significance, Yukio Suzuki and Kei Uchida. Enzymatic Synthesis of Glycosides in Aqueous-Organic Two-Phase Systems and Supersaturated Substrate Solutions, Jeffrey A. Khan, Anna Millqvist-Fureby, and Evgeny N. Vulfson. Use of b-Glucosidase in the Development of Flavor in Wines and Fruit Juices, Yannick Gueguen, Patrick Chemardin, Guilhem Janbon, Alain Arnaud, and Pierre Galzy. Index.

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