Cardiovascular Physiology in the Genetically Engineered Mouse / Edition 2

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Overview

The enormous advances in molecular biology and genetics coupled with the progress in instrumentation and surgical techniques have produced a voluminous and often bewildering quantity of data. The primary objective of a second edition of Cardiovascular Physiology in the Genetically Engineered Mouse is to interpret critically the literature and to provide a framework for the enormous amount of information in this burgeoning field. As in the first edition, the monograph serves as a practical guide for the investigator interested in the functional methods used to characterize the murine cardiovascular phenotype.
The monograph is organized into three parts. The first deals with principles of transgenesis and homologous recombination. The second part, which again is the largest, discusses the various techniques used to assess the cardiovascular mechanical, metabolic, and electrophysiologic phenotype. This section is organized in a hierarchical manner- i.e. from isolated myocyte to isolated heart to the intact, anesthetized and conscious mouse. The third part examines techniques used to evaluate murine smooth muscle function, genetic mouse models of hypertrophy and heart failure, and the methods to assess the cardiovascular phenotype in the developing mouse embryo. In addition, newer methods that 'push the envelope', such as magnetic resonance imaging (MRI), positron emission tomography (PET), ultrasound biomicroscopy (UBM), and computed tomography (microCT) are discussed. Expanded and updated, each chapter is richly enhanced with original tables and figures, and in many cases, extensively rewritten when compared with the first edition.
An essential and enduring goal of this second edition is to continue to facilitate interactions between the basic science disciplines and help bridge the gap between molecular biology and physiology.

The book contains black-and-white illustrations.

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Editorial Reviews

Doody's Review Service
Reviewer: Kerby C. Oberg, MD, PhD (Loma Linda University)
Description: This book couples an in-depth update on current techniques to evaluate cardiac physiology with an introduction to the molecular mechanisms used to engineer alterations in the mouse genome.
Purpose: Genetic alteration of cardiac-related genes in mice provides a powerful tool to unravel the mechanisms behind cardiac development, cardiac function, and the pathophysiology of cardiac disease. To fully appreciate the anatomic and physiologic effects of these genetically engineered mice, new and classical techniques of analysis must be employed.
Audience: This book is written for those facing the challenge of analyzing the cardiac sequelae of altering cardiac-related genes in mice. Although this might include molecular biologists attempting to analyze new mouse mutants developed in their laboratories, its language and focus target those whose primary discipline is physiology in general and cardiac physiology in particular (as the title indicates). For example, the early chapters introducing the molecular techniques employed in genetic engineering provide a well-written, but brief, overview for the classical physiologist or medical clinician who may not have any experience or training in molecular techniques. As physician-scientists who are well published in both scientific and clinical literature, both authors bring a unique bent toward translational medicine to the book
Features: The authors of this book attempt to provide a common resource for molecular biologists, physiologists, and to some extent cell biologists in an effort to enhance productivity in this field and encourage collaborative efforts. The book falls short of being comprehensive in that it fails to include any directed discussion about techniques or an approach to study potential morphologic subtleties of hearts from genetically engineered mice. That said, the book still provides a forum for integrating classical and molecular approaches.
Assessment: This book is a timely resource at a point in science where genetically engineered mice are being produced more rapidly than they can be analyzed. Although several symposia have focused on the pathology of genetically-engineered mice (GEM) including that of cardiovascular pathology, no book or symposium has targeted cardiac pathophysiology of the GEM with the depth that this book does. Furthermore, this second edition adds much needed introductory material including molecular techniques, cardiac development and cutting-edge technologies.
From The Critics
Reviewer: Kerby C. Oberg, MD, PhD(Loma Linda University)
Description: This book couples an in-depth update on current techniques to evaluate cardiac physiology with an introduction to the molecular mechanisms used to engineer alterations in the mouse genome.
Purpose: Genetic alteration of cardiac-related genes in mice provides a powerful tool to unravel the mechanisms behind cardiac development, cardiac function, and the pathophysiology of cardiac disease. To fully appreciate the anatomic and physiologic effects of these genetically engineered mice, new and classical techniques of analysis must be employed.
Audience: This book is written for those facing the challenge of analyzing the cardiac sequelae of altering cardiac-related genes in mice. Although this might include molecular biologists attempting to analyze new mouse mutants developed in their laboratories, its language and focus target those whose primary discipline is physiology in general and cardiac physiology in particular (as the title indicates). For example, the early chapters introducing the molecular techniques employed in genetic engineering provide a well-written, but brief, overview for the classical physiologist or medical clinician who may not have any experience or training in molecular techniques. As physician-scientists who are well published in both scientific and clinical literature, both authors bring a unique bent toward translational medicine to the book
Features: The authors of this book attempt to provide a common resource for molecular biologists, physiologists, and to some extent cell biologists in an effort to enhance productivity in this field and encourage collaborative efforts. The book falls short of being comprehensive in that it fails to include any directed discussion about techniques or an approach to study potential morphologic subtleties of hearts from genetically engineered mice. That said, the book still provides a forum for integrating classical and molecular approaches.
Assessment: This book is a timely resource at a point in science where genetically engineered mice are being produced more rapidly than they can be analyzed. Although several symposia have focused on the pathology of genetically-engineered mice (GEM) including that of cardiovascular pathology, no book or symposium has targeted cardiac pathophysiology of the GEM with the depth that this book does. Furthermore, this second edition adds much needed introductory material including molecular techniques, cardiac development and cutting-edge technologies.
Booknews
A how-to guide for investigators interested in functional methods used to characterize the murine cardiovascular phenotype, for both cardiovascular physiologists and molecular biologists. Section I deals with transgenic and gene targeting approaches to cardiovascular physiology and pathology. Section II discusses techniques used to assess the cardiovascular phenotype, including isolated myocyte mechanics and calcium transients, NMR spectroscopy, and invasive hemodynamics. Section III examines methods and techniques used to study cardiovascular development, exercises, and oxygen consumption , and the role of genetic manipulations in the study of the vascular system. Annotation c. by Book News, Inc., Portland, Or.

3 Stars from Doody
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Product Details

  • ISBN-13: 9780792375364
  • Publisher: Springer US
  • Publication date: 10/31/2001
  • Series: Developments in Cardiovascular Medicine Series , #238
  • Edition description: 2nd ed. 2001
  • Edition number: 2
  • Pages: 403
  • Product dimensions: 1.00 (w) x 6.14 (h) x 9.21 (d)

Table of Contents

Preface. Part One. 1. The Hierarchical Approach to Analysis of Cardiovascular Function in Mice: An Overview; R.A. Walsh. 2. Genetic Alterations and Modeling of Cardiovascular Physiology; A. Schmidt, et al. 3. Conditional Transgenesis; T. Minamino, et al. 4. Array Transcription Profiling: Molecular Phenotyping of Rodent Cardiovascular Models; S. Acton, et al. 5. Murine Cardiovascular Development; S.A. Fisher. Part Two. 6. Isolated Myocyte Mechanics and Calcium Transients; Z. Su, et al. 7. Single Cell Patch-Clamp Analysis of Mouse Cardiac Myocytes; N. Chiamvinonvat, et al. 8. The Isolated Isovolumic (Langendorff) Heart Preparation; M. LeWinter. 9. The Isolated Work-Performing and Ejecting Mouse Heart Preparation, Comparison and Quantification of Cardiac Performance in Transgenic and Wild-type Mice; I.L. Grupp, et al. 10. 31P NMR Spectroscopy of the Mouse Heart; J.S. Ingwall, et al. 11. Electrophysiological Characterization of the Mouse Heart Using Voltage-Sensitive Dyes; B.C. Eloff, D. Rosenbaum. 12. Echocardiographic Assessment of the Mouse Heart and Aorta; B.D. Hoit. 13. In Vivo Invasive Hemodynamic Studies in Mice; B.D. Hoit. 14. Pressure-Volume Relations; D. Georgakopoulos, D.A. Kass. 15. Assessment of Left Ventricular Diastolic Function in Mice; M.R. Zile, et al. 16. In Vivo Electrophysiologic Studies in Mice; C.I. Berul, et al. 17. Cardiovascular Physiology in Mice: Conscious Function Measurements and Effects of Anesthesia;S. Vatner, et al. 18. Exercise and Oxygen Consumption in the Mouse; K.H. Desai, D. Bernstein. 19. Clinical Laboratory Analysis of the Genetically Manipulated Mouse; W. Lewis. Part Three. 20. Smooth Muscle Studies Using Gene-Altered Mouse Models: A Users' Guide; R.L. Sutliff, R.J. Paul. 21. Models of Cardiac Disease in the Mouse, Cardiac Hypertrophy and Heart Failure; Y. Ikeda, J. Ross, Jr. 22. Analysis of Murine Embryonic Cardiovascular Phenotype; K. Tobita, et al. 23. Newer In Vivo Imaging Modalities; R.S. Lazebnik, D.L. Wilson. Index.

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