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Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world, present a collection of the state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both the novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays.
Comprehensive and cutting-edge, Celiac Disease: Methods and Prools provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.
The book contains black-and-white illustrations.
Celiac Disease: A Brief Overview, Debbie Williamson and Michael N. Marsh. Genotyping Methodologies, Stephen Bevan and Richard S. Houlston. From Linkage to Genes: Positional Cloning, Stephen Bevan and Richard S. Houlston. Linkage and the Transmission Disequilibrium Test in Complex Traits: Celiac Disease as a Case Study, Stephen Bevan and Richard S. Houlston. Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats, Arthur S. Tatham, Simon M. Gilbert, Roger J. Fido, and Peter R. Shewry. Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry, Peter A. van Veelen, Jan W. Drijfhout, and Frits Koning. Synthetic Peptide Libraries for T-Cell Epitope Identification, Hoebert S. Hiemstra, Jan W. Drijfhout, and Frits Koning. Characterization of HLA-DQ-Specific Peptide-Binding Motifs, Yvonne van de Wal, Reinout Amons, and Frits Koning. Studies of Gliadin-Specific T Cells in Celiac Disease, Øyvind Molberg, Stephen N. McAdam, Knut E. A. Lundin, and Ludvig M. Sollid. Morphometric Analysis of Intestinal Mucosa: The Measurement of Volume Compartments and Cell Volumes in Human Intestinal Mucosa, Michael N. Marsh, Peter T. Crowe, Kieran J. Moriarty, and Arzu Ensari. Morphometry of Rectal Mucosa, Arzu Ensari, Michael N. Marsh, Peter T. Crowe, and Kieran J. Moriarty. Organ Culture of Rectal Mucosa: In Vitro Challenge with Gluten in Celiac Disease, Guiseppe Mazzarella, Franco Paparo, Maria Maglio, and Riccardo Troncone. In Situ Hybridization, Conrad G. Beckett and Paul Ciclitira. Quantification of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease, Ellen M. Nilsen, Finn-Eirik Johansen, and Per Brandtzaeg. Immunohishemistry in Research and Diagnosis of Celiac Disease, Per Brandtzaeg and Frode L. Jahnsen. Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease, Walburga Dieterich, Ernst Otto Riecken, and Detlef Schuppan.Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease, David J. Unsworth. Whole Gut Lavage Fluid Analysis: A Minimally Invasive Method for Study of Mucosal Immunity and Inflammation, Subrata Ghosh, Anna Dahele, Hazel E. Drummond, Syed S. Hoque, Kenneth Humphreys, and Ian D. R. Arnott. Index.