Celiac Disease: Methods and Protocols / Edition 1

Paperback (Print)
Buy New
Buy New from BN.com
Used and New from Other Sellers
Used and New from Other Sellers
from $106.06
Usually ships in 1-2 business days
(Save 28%)
Other sellers (Paperback)
  • All (7) from $106.06   
  • New (5) from $106.06   
  • Used (2) from $187.13   


Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world, present a collection of the state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both the novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays.

Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.

The book contains black-and-white illustrations.

Read More Show Less

Editorial Reviews

From the Publisher
"Of interest to gastroenterologists, pathologists and those working in research on celiac disease, a disease linked by a so far unknown mechanism to type 1 diabetes." -Journal of Pediatric Endocrinology & Metabolism
Research gastroenterologists, immunologists, and other scientists detail laboratory methods for studying the biology and immunopathology of celiac disease, which involves sensitivity to gluten. They include reproducible techniques for cloning lymphocytes, using physical methods to identify gluten sequences as T-cell antigens, studying peptide responses and by either jejunal or rectal challenge, elucidating the locations of other genes concerned in pathogenesis, and using elegant immuno-histo-cyto-chemical and mRNA probing techniques for analyzing the finer points of mucosal inflammatory response to gluten. Gluten sensitivity research is gaining popularity, and the descriptions here should allow newcomers to begin with the current methods. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Read More Show Less

Product Details

  • ISBN-13: 9781617371158
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/9/2010
  • Series: Methods in Molecular Medicine Series, #41
  • Edition description: Softcover reprint of hardcover 1st ed. 2000
  • Edition number: 1
  • Pages: 288
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.68 (d)

Table of Contents

Celiac Disease: A Brief Overview, Debbie Williamson and Michael N. Marsh. Genotyping Methodologies, Stephen Bevan and Richard S. Houlston. From Linkage to Genes: Positional Cloning, Stephen Bevan and Richard S. Houlston. Linkage and the Transmission Disequilibrium Test in Complex Traits: Celiac Disease as a Case Study, Stephen Bevan and Richard S. Houlston. Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats, Arthur S. Tatham, Simon M. Gilbert, Roger J. Fido, and Peter R. Shewry. Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry, Peter A. van Veelen, Jan W. Drijfhout, and Frits Koning. Synthetic Peptide Libraries for T-Cell Epitope Identification, Hoebert S. Hiemstra, Jan W. Drijfhout, and Frits Koning. Characterization of HLA-DQ-Specific Peptide-Binding Motifs, Yvonne van de Wal, Reinout Amons, and Frits Koning. Studies of Gliadin-Specific T Cells in Celiac Disease, Øyvind Molberg, Stephen N. McAdam, Knut E. A. Lundin, and Ludvig M. Sollid. Morphometric Analysis of Intestinal Mucosa: The Measurement of Volume Compartments and Cell Volumes in Human Intestinal Mucosa, Michael N. Marsh, Peter T. Crowe, Kieran J. Moriarty, and Arzu Ensari. Morphometry of Rectal Mucosa, Arzu Ensari, Michael N. Marsh, Peter T. Crowe, and Kieran J. Moriarty. Organ Culture of Rectal Mucosa: In Vitro Challenge with Gluten in Celiac Disease, Guiseppe Mazzarella, Franco Paparo, Maria Maglio, and Riccardo Troncone. In Situ Hybridization, Conrad G. Beckett and Paul Ciclitira. Quantification of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease, Ellen M. Nilsen, Finn-Eirik Johansen, and Per Brandtzaeg. Immunohishemistry in Research and Diagnosis of Celiac Disease, Per Brandtzaeg and Frode L. Jahnsen. Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease, Walburga Dieterich, Ernst Otto Riecken, and Detlef Schuppan.Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease, David J. Unsworth. Whole Gut Lavage Fluid Analysis: A Minimally Invasive Method for Study of Mucosal Immunity and Inflammation, Subrata Ghosh, Anna Dahele, Hazel E. Drummond, Syed S. Hoque, Kenneth Humphreys, and Ian D. R. Arnott. Index.

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)