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Cell-Cell Interactions: Methods and Protocols

Overview

Inthe second edition of Cell-Cell Interactions: Methods and Prools,expert researchers in the field detail a collection of prools to examine interactions between cells. These include prools that focus on bothin vivoandin vitromethods-based approaches. New updated chapters also bring together many currently used assays in examining cell-cell interactions and the biological consequences of those interactions. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to...

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Overview

Inthe second edition of Cell-Cell Interactions: Methods and Prools,expert researchers in the field detail a collection of prools to examine interactions between cells. These include prools that focus on bothin vivoandin vitromethods-based approaches. New updated chapters also bring together many currently used assays in examining cell-cell interactions and the biological consequences of those interactions. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and Practical,Cell-Cell Interactions: Methods and Prool, Second Editionis a valuable resource for researchers who are already involved in the cell-cell interaction field, and also for thosethat are new to the area.

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Product Details

  • ISBN-13: 9781627036030
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 9/2/2013
  • Series: Methods in Molecular Biology Series , #1066
  • Edition description: 2nd ed. 2013
  • Edition number: 2
  • Pages: 204
  • Product dimensions: 6.90 (w) x 10.00 (h) x 0.70 (d)

Table of Contents

1. Proteomics Analysis of Contact-initiated Eph Receptor-ephrin Signaling

Claus Jorgensen and Alexei Poliakov

2. Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Cells and Pericytes

George E. Davis, Dae Joong Kim, Chun-Xia Meng, Pieter R. Norden, Katherine R. Speichinger, Matthew T. Davis, Annie O. Smith, Stephanie L.K. Bowers, and Amber N. Stratman

3. Analyzing Cell-cell Interactions in 3-dimensional Adhesion Assays

Stephanie L.K. Bowers and Troy A. Baudino

4. Production of Spontaneously Beating Neonatal Rat Heart Tissue for Calcium and Contractile Studies

FNU Gerilechaogetu, Hao Feng, Honey B. Golden, Damir Nizamutdinov, Donald M. Foster, Shannon Glaser, and David E. Dostal

5. Paracrine Communication Between Mechanically Stretched Myocytes and Fibroblasts

Hao Feng, FNU Gerilechaogetu, Honey B. Golden, Damir Nizamutdinov, Donald M. Foster, Shannon Glaser, and David E. Dostal

6. Assessing Blood Brain Barrier Function using in vitro Assays

Joseph Bressler, Katherine Clark, and Cliona O’Driscoll

7. Methods to Assess Tissue Permeability

Juan C. Ibla and Joseph Khoury

8. In vivo Quantification of Metastatic Tumor Cell Adhesion in the Pulmonary Microvasculature

F Bartsch, ML Kang, ST Mees, J Haier, and P Gassmann

9. Cell Membrane Vesicles as a Tool for the Study of Direct Epithelial-stromal Interaction: Lessons from CD147

Eric Gabison, Farah Khayati, Samia Mourah, and Suzanne Menashi

10. Microencapsulation of Stem Cells to Study Cellular Interactions

Keith Moore, Adam Vandergriff, and Jay D. Potts

11. Cell-surface Protein-protein Interaction Analysis with Time-resolved FRET and Snap-tag Technologies

Timothy N. Feinstein

12. Single Cell Analysis of Lipid Rafts

William T. Lee

13. Micropatterning Cell Adhesion on Polyacrylamide Hydrogels

Jian Zhang, Wei-hui Guo, Andrew Rape, and Yu-li Wang

14. Measuring Cell-cell Tugging Forces using Bowtie-patterned mPADs (Microarray Post Detectors)

Daniel M. Cohen, Mike T. Yang, and Christopher S. Chen

15. Generation and Analysis of Biosensors to Measure Mechanical Forces within Cells

Katharina Austen, Carleen Kluger, Andrea Freikamp, Anna Chrostek‑Grashoff, and Carsten Grashoff

16. Proteomic Analysis of the Left ventricle Post-myocardial Infarction to Identify in vivo Candidate Matrix Metalloproteinase Substrates

Andriy Yabluchanskiy, Yaojun Li, Lisandra E. de Castro Brás, Kevin Hakala, Susan T. Weintraub, and Merry L. Lindsey

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