Cell Cycle - Materials and Methods / Edition 1

Paperback (Print)
Buy New
Buy New from BN.com
Used and New from Other Sellers
Used and New from Other Sellers
from $190.73
Usually ships in 1-2 business days
(Save 26%)
Other sellers (Paperback)
  • All (7) from $190.73   
  • New (6) from $173.28   
  • Used (1) from $334.81   


During their lifetime, especially when growing and dividing, cells go through various steps of the cell cycle. Knowledge of the individual steps of the cell cycle will help us understand the development of a variety of diseases better, including cancer, and also to design new drugs against it. New techniques for studying the molecular basis of these processes have recently been developed and are described in detail in this manual.
A glossary helps the reader to cope with the complex cell cycle terminology.

Read More Show Less

Editorial Reviews

From the Publisher
"Newcomers to cell-cycle research will enjoy and profit greatly from reading this new manual." Trends in Cell Biology
"...a valuable desk reference for scientists and students. While several other books describing prools for investigation of growth control have recently been published, this is among the most viable." FEBS Letters
Read More Show Less

Product Details

  • ISBN-13: 9783540580669
  • Publisher: Springer Berlin Heidelberg
  • Publication date: 12/12/1995
  • Series: Springer Lab Manuals Series
  • Edition description: 1st Corrected ed. 1996. Corr. 2nd printing 0
  • Edition number: 1
  • Pages: 287
  • Product dimensions: 7.44 (w) x 9.69 (h) x 0.67 (d)

Table of Contents

One Techniques for Studying the Mammalian Cell Cycle.- 1 Use of Immunohishemistry in Cell Cycle Studies.- 2 Immunofluorescence Techniques in Cell Cycle Studies.- 3 Fluorescence in Situ Hybridization and Human Cell Cycle Genes.- 4 Techniques for Monitoring Cell Cycle Phases.- 5 Centrifugal Elutriation.- 6 Synchronization of Mammalian Cells.- 7 Microinjection and Electroporation of Macromolecules into Live Cells.- 8 Stable and Transient Transfections in Cell Cycle Studies.- 9 Quantification of Premature and Normal Mitosis.- Two Techniques for Studying the Xenopus Cell Cycle.- 10 Preparation of Xenopus Egg Extracts and Their Utilization in Cell Cycle Studies.- Three Techniques to Study the Yeast Cell Cycle.- 11 Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe.- 12 Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces Pombe.- Four Techniques for Studying the Cell Cycle of Any Species.- 13 Identification of Novel Cell Cycle Targets Using Small Molecule Ligands.- 14 Bacterial Expression and in Vitro Assay of Cell Cycle Kinases.- 15 Heterologous Expression and Secretion of Cell Cycle Proteins Using the Yeast Pichia pastoris.- 16 SV40 Based in Vitro DNA Replication Assay.- 17 From Sequence to Function-Structural Motifs of Cell Cycle Proteins.- 18 Purification of Cell Cycle Kinases.- 19 Interaction-Based Cloning of Novel Proteins.- 20 Expression of Cell Cycle Proteins Using Recombinant Baculoviruses.- 21 Cell Cycle-Regulated Transcription Factors: Bandshift Assays.- 22 Immunoprecipitation and Immunoblotting in Cell Cycle Studies.- 23 Protein mapping in Cell Cycle Studies.- 24 From Peptide to Purified Antibody.

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)