Cell Separation Methods and Applications / Edition 1

Cell Separation Methods and Applications / Edition 1

by Diether Recktenwald
     
 

"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "See more details below

Overview

"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "

Product Details

ISBN-13:
9780824798642
Publisher:
Taylor & Francis
Publication date:
11/28/1997
Edition description:
New Edition
Pages:
352
Product dimensions:
6.25(w) x 9.25(h) x 0.75(d)

Table of Contents

Background: historical and useful methods of preselection and preparative scale sorting. Physical methods of separation: new approaches in density gradient separation using colloidal silica; gradients in the processing of human haematopoietic progenitor cells; centrifugal elutriation - a powerful separation technique in cell biology immunology, and haematology. Antibody-based methods: isolation, activation, expansion, and gene transduction of cell-based therapeutics using polystyrene immunoaffinity devices; the CEPRATER SC system - technology, clinical development, and future directions; high density particles - a novel, highly efficient cell separation technology; antibody and complement mediated cell separation. Magnetic methods: magnetic cell sorting with colloidal superparamagnetic particles; immunomagnetic cell separation using antibodies and superparamagnetic microspheres; free flow magnetophoresis - continuous immunomagnetic sorting of cells and organelles by magnetic deviation and focusing. Flow cytometry: cell separation using flow cytometric cell sorting. Special applications: employing surface markers for the selection of transfected cells; CD34 cell; sorting and enrichment - applications in blood banking and transplantation. Appendices: cellular properties for cell separation; CD antigen designations. Glossary.

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