Cell Separation Methods and Applications / Edition 1

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"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "

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Editorial Reviews

Describes methods for the analytical and preparative isolation of specific populations of biological cells. Covers basic methods such as lytic removal of cell subsets, and details new developments in methods using endogenous physical cell properties such as cell size and cell density. Describes more specific cell separation methods based on monoclonal antibodies against cell surface markers, set out basic technical principles of the methods, and discusses typical examples with performance data, looking at applications in basic research and medicine. Includes an appendix of cell properties for cell separations and CD antigens. For those involved in clinical cellular therapy for transplantation, cancer, and AIDS, and in gene therapy. Annotation c. by Book News, Inc., Portland, Or.
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Product Details

  • ISBN-13: 9780824798642
  • Publisher: Taylor & Francis
  • Publication date: 11/28/1997
  • Edition description: New Edition
  • Edition number: 1
  • Pages: 352
  • Product dimensions: 6.25 (w) x 9.25 (h) x 0.75 (d)

Table of Contents

Background: historical and useful methods of preselection and preparative scale sorting. Physical methods of separation: new approaches in density gradient separation using colloidal silica; gradients in the processing of human haematopoietic progenitor cells; centrifugal elutriation - a powerful separation technique in cell biology immunology, and haematology. Antibody-based methods: isolation, activation, expansion, and gene transduction of cell-based therapeutics using polystyrene immunoaffinity devices; the CEPRATER SC system - technology, clinical development, and future directions; high density particles - a novel, highly efficient cell separation technology; antibody and complement mediated cell separation. Magnetic methods: magnetic cell sorting with colloidal superparamagnetic particles; immunomagnetic cell separation using antibodies and superparamagnetic microspheres; free flow magnetophoresis - continuous immunomagnetic sorting of cells and organelles by magnetic deviation and focusing. Flow cytometry: cell separation using flow cytometric cell sorting. Special applications: employing surface markers for the selection of transfected cells; CD34 cell; sorting and enrichment - applications in blood banking and transplantation. Appendices: cellular properties for cell separation; CD antigen designations. Glossary.

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