Cell Technology for Cell Products: Proceedings of the 19th ESACT Meeting, Harrogate, UK, June 5-8, 2005 / Edition 1

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Overview

The 19th ESACT meeting was to highlight the novel capabilities of the industry to move the products towards the clinic. It was attended by a wide range of workers in the industry and for many it was their first ESACT meeting. The proceedings here include the short papers adding the knowledge of the previous meetings and provide a reference for the researcher entering, or continuing in the field of Animal Cell Technology.

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Product Details

  • ISBN-13: 9781402054754
  • Publisher: Springer Netherlands
  • Publication date: 3/12/2007
  • Series: ESACT Proceedings Series , #3
  • Edition description: 2007
  • Edition number: 1
  • Pages: 824

Table of Contents

Contents; 19th ESACT Meeting Committees; ESACT Executive Committee; Sponsors; Companies in Trade Exhibition; Acknowledgements; List of Participants; CHAPTER I: PERSPECTIVES OF THE BIOTECH INDUSTRY; The UK bioprocessing sector, Tony Bradshaw; CHAPTER II: TRANSCRIPTION TO SECRETION; A Novel AIR-induced Gene Expression System in HEK.EBNA Cells and its Applications, N.S. Werner et al.; Towards Stronger Gene Expression - a Promoter's Tale, S. Kalwy et al.; Improving the Expression of a Soluble Receptor: Fc fusion Protein in CHO Cells by Coexpression with the Receptor Ligand, G.W. Lee et al.; Growth, Apoptosis and Functional Genomics Analysis of CHO-K1 Over-Expressing Telomerase, F. Crea et al.; Microarray Analysis of Metabolically Engineered NS0 Cell Lines Producing Chimeric Antibody, G. Khoo et al.; Optimizing Medium for Transient Transfection, Z.-H. Geng et al.; Establishment of Recombinant CHO Cell Lines Under Serum-free Conditions, M. Kuchenbecker et al.; Novel Cell-based Assay for the Detection of Murine type I IfN, M. Bollati-Fogolín et al.; Characterisation of the Induction of Cyhrome p450 Enzymes in Primary Cultures of Human Hepaytes, T.L. Freeman et al.; Effect of Culture Conditions on Glycosylation of Recombinant beta-Interferon in CHO Cells, M. Spearman et al.; Induction of Caspase-3-dependent Apoptosis by Electrolyzed Reduced Water/platinum Nanoparticles in Cancer Cells, T. Hamasaki et al.; Molecular Mechanism of TAK1-Induced Repression of hTERT Transcription, M. Maura, et al.; Analysis of Genetic Parameters in Order to Get More Information on High Producing Recombinant CHO Cell Lines, C. Lattenmayer et al.; Redox Regulation by Reduced Waters as Active Hydrogen Donors and Intracellular ROS Scavengers for Prevention of type 2 Diabetes, S. Shirahata et al.; Transcription Profiling of Different Recombinant CHO Clones Based on Cross-species Microarray Analysis, E. Trummer et al.; Adaptation without Cell Division? K.L. Astley et al.; Release of Plasmid-DNC from PEI/DNA Particles, M. Bertschinger et al.; Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody, S. Matsumoto et al.; Design Approach Regarding Humanization and Functionality of an Anti-CD18 Monoclonal Antibody, C.A. Caldas et al.; Development of a Transient Mammalian Expression Process for the Production of the Cancer Testis Antigen NY-ESO-1, D.L. Hacker et al.; The Role of the Sumoylation Pathway in Transient and Stable Recombinant Protein Expression in Chinese Hamster Ovary Cells, M. Bertschinger et al.; Effect of Different Materials Used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium, P. Aizawa et al.; Antibody Production by GS-CHO Cell Lines Over Extended Culture Periods, A.J. Porter et al.; Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells, M. Schomberg et al.; Effect of the Scaffold in Maintenance of Suitable Chondrcyte Morphology in Cartilage Regeneration, J. Rodríguez et al.; Metabolic Changes Occuring During Adaptation to Serum Free Media, S. Denby et al.; The Expression of the neo gene in GS-NS0 Cells Increases their Proliferative Capacity, F. Veraitch et al.; CHAPTER III: THERAPEUTIC CELL ENGINEERING; The Therapeutic Potential of Human Embryonic Stem Cells, S.H. Cedar et al.; Investigations of Murine Embryonic Stem Cell Maintenance by Analyses of Culture Variables and Gene Expression, J.M. Piret et al.; Generation of Cardiomyocytes Derived from Mouse ESC in a Scale-up System, S. Niebruegge et al.; Expansion of Undifferentiated Human Embryonic Stem Cells, A.B.H. Choo et al.; New Electrofusion Devices for the Improved Generation of Dendritic Cell-tumour Cell Hybrids, J. Schaper et al.; Influence of c-Myc and bcl-2 CO-expression on Proliferation, Apoptosis and Survival in CHO Cultures, V. Ifandi et al.; Ex Vivo Expansion of Hematopoietic Stem Cells Using Defined Culture Media, J. Hartshorn et al.; Development of A Novel Serum-free Cryopreservative Solution, T. Toyosawa et al.; Hepatic Cell Lines CultureD on Different Scaffolds and in Different Stages for Bioartificial Liver Systems, K. Kaito et al.; Comparison of Flask and Bag Systems in Cultivation of Dendritic Cells for Clinical Application, L. Macke et al.; T-Lymphocytes Transduced with SFCMM-3 Vector in a Closed System, C. Benati et al.; Influence of Down-regulation of Caspase-3 and CASPASE-7 by Sirnas on Sodium Butyrate-induced Apoptotic Cell Death of Chinese Hamster Ovary Cells Producing Thrombopoietin, Y.H. Sung et al.; CHAPTER IV: GENE MEDICINE; UTRtech™: Exploiting mRNA Targeting To Increase Protein Secretion From Mammalian Cells, B. Stern et al.; Semi-Synthetic Mammalian Gene Regulatory Networks, B.P. Kramer et al.; Acoustic Cell Processing for Viral Transduction or Bioreactor Cell Retention, V.M. Gorenflo et al.; Comparison of Different Transient Retroviral Transfection Systems, V. Kleff et al.; CHAPTER V: CELLS TO TISSUES; Artificial Human Lymphnode, C. Giese et al.; Single Cell Study in a Hydrogel, T. Braschler et al.; Predicting Tissue Assembly of Prostate Cancer Spheroids, K. O’Connor et al.; Cultivating Cells of Different Origin for 3d Bone Constructs Considering Physiological Conditions, K. Suck et al.; Guidance on Good Cell Culture Practice, S. Coecke et al.; In Vitro Vascularization of Human Microtissues, J.M. Kelm et al.; Preparation of Salmon Atelocollagen Fibrillar Gel and Its Application to the Cell Culture, N. Nagai et al.; Propagation of USSC for Therapeutic Approaches, J. Lamlé et al.; Bioreactor Based Production of Functional Conditioned Medium for the Propagation of Undifferentiated Human Embryonic Stem Cells, A. Bauwens et al.; Large-scale Expansion of Cells for Use as Therapeutic Agents, J. Melero-Martin et al.; EX VIVO Expansion of CD34+ Cells in Stemline. I I Hematopoietic Stem Cell Expansion Medium Generates a Large Population of Functional Early and Late Progenitor Cells, S.L. Leugers et al.; In Vitro Bone Formation of Multipotential Human Mesenchymal Cells in Fixed Bed Perfusion Bioreactors, N. Zghoul et al.; Influence of Basal Media Formulations and Calcium on Growth and Directed Differentiation of Human Osteogenic Cells In Vitro, I. Majore et al.; CHAPTER VI: PROTEIN PRODUCTS; BHRF-1 as a Tool for Genetic Inhibition of Apoptotis in Hybridoma Cell Cultures, S. Juanola et al.; Karyotype of CHO DG44 cells, D. Martinet et al.; From Biopsy to Cartilage-carrier-constructs by Using Microcarrier Cultures as Sub-process, S. Nagel-Heyer et al.; Effect of Peptones and Study of Feeding Strategies in a CHO Based Fed-batch Process for the Production of a Human Monoclonal Antibody, V. Chotteau et al.; Retinoic Acid Induced Up-regulation of Monoclonal Antibody Production, Y. Inoue et al.; Media and Cell Manipulation Approaches to the Reduction of Adaptation Time in GS NS0 Cell Line, P. Clee et al.; The Production of Human Growth Hormone, F. Anton et al.; Preparation of Membrane Receptor Proteins Utilising Large Scale Micro-carrier Culture and a "Pearl Freezing Factory" to Finalise the Product, I. Jansson et al.; Silk Protein Sericin Improves Mammalian Cell Culture, S. Terada et al.; Design of Culture Media for Drosophila Melanogaster S2 Cells Producing Recombinant G Glycoprotein from Rabies Virus, A.L.L. Galesi et al.; Comparison of the Cultivation of Wild and Transfected Drosophila Melanogaster S2 Cells in Different Media, K. Swiech et al.; Low Temperature Culture Effects on sCR1 Productivity by rCHO Cells, H. Matsuoka et al.; Proteomic Characterization of Recombinant NS0 Cell Clones Obtained by an Integrated Selection Strategy, A. Castillo et al.; IFN-Glycosylation Macroheterogeneity and Intracellular Nucleotide and Sugar Nucleotide Contents of CHO Cells Cultivated in Batch Culture, N. Kochanowski et al.; Influence of the Adaptation Procedure and the Serum-free Conditions on the N-glycosylation Pattern of EPO Produced by CHO Cells, F. Le Floch et al.; Stability and Cytogenetic Characterization of Recombinant CHO Cell Lines Established by Microinjection and Calcium Phosphate Transfection, M. Derouazi et al.; Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody, S. Matsumoto et al.; Design Approach Regarding Humanization and Functionality of an Anti-CD18 Monoclonal Antibody, C.A. Caldas et al.; Development of a Transient Mammalian Expression Process for the Production of the Cancer Testis Antigen NY-ESO-1, D.L. Hacker et al.; Effect of Different Materials used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium, P. Aizawa et al.; Antibody Production by GS-CHO Cell Lines Over Extended Culture Periods, A.J. Porter et al.; Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells, M. Schomberg et al.; Effect of the Scaffold in Maintenance of Suitable Chondrcyte Morphology in Cartilage Regeneration, J. Rodríguez et al.; Metabolic Changes Occuring During Adaptation to Serum Free Media, S. Denby et al.; The Expression of the neo Gene in GS-NS0 Cells Increases Their Proliferative Capacity, F. Veraitch et al.; 1000 Non-instrumented Bioreactors in a Week, M. Stettler et al.; Optimisation of Time-space yield for Hybridoma fed-batch Cultures with an Adaptive Olfo-controller, R. Pörtner et al.; Influenza Vaccines – Challenges in Mammalian Cell Culture Technology, Y. Genzel et al.; Towards pCO2 -optimised Fermentations – Reliable Sampling, pCO2 Control & Cellular Metabolism, A. Jockwer et al.; Cultivation of E-FL in Perfusion Mode Using Wave® Bioreactor and Stirred-Tank Reactor for Parvovirus Vaccine Production, B. Hundt et al.; Repeated Batch Fermentation Using a Viafuge Centrifuge, K. Russ et al.; Serum-free Transient Expression of Antibodies Using 40 Tray Cell Factories, P. Schlenke et al.; Monitoring Cell Physiology in Influenza Vaccine Production by Flow Cytometry, J. Schulze-Horsel et al.; NucleoCounter – An Efficient Technique for the Determination of Cell Number and Viability in Animal Cell Culture Processes, D. Shah et al.; On line Biomass Monitoring in Cell Culture with Scanning Radio-frequency Impedance Spectroscopy, J.P. Carvell et al.; The Use of a Mobile Robot for Complete Sample Management in a Cell Culture Pilot Plant, M. Wojtczyk et al.; Closed Loop Control of Perfusion Systems in High-density Cell Culture, A. Bock et al.; Optimization of Culture Medium with the Use of Protein Hydrolysates, P. Ganglberger et al.; Optimization of a Fed-batch Strategy for Production of Mab Ign 311 in Small Scale Experiments, P. Hinterleitner et al.; Serum-free Influenza Vaccine Production with MDCK Cells in Wave-bioreactor and 5L-stirred Tank Bioreactor, Y. Genzel et al.; Rotary Bioreactor for Recombinant Protein Production, S. Navran; Flow Cytometry as a Tool for Process Monitoring of Virus-cell Systems, K.S. Sandhu et al.; The Use of Multi-parameter Flow Cytometry for Characterisation of Insect Cell-baculovirus Cultures, B. Isailovic et al.; Understanding Metabolic Needs of EB14 Cells in Culture, J. Hartshorn et al.; Virus Production in Vero Cells Using a Serum-free Medium, K. Kolell et al.; Computational Fluid Dynamics Applied to Laboratory Scale Cell Culture Reactors for the Prediction of Hydrodynamic Stress, E. Olmos et al.; Using Genomic Tools for the Identification of Important Signaling Pathways in Order to Facilitate Cell Culture Medium Development, D.W. Allison et al.; Development and Application of An Animal-component Free Single-cell Cloning Medium for Chinese Hamster Ovary Cell Lines, N. Lin et al.; Plant Peptones: Nutritional Properties Sustaining Recombinant Protein Secretion by CHO Cells, J. Mols et al.; Monitoring of Extracellular TCA Cycle Intermediates in Mammalian Cell Culture, J.B. Ritter et al.; A Comparison of the SixFors Fermenter System and Lab Scale Fermenters For Clone Selection, G. Wilson et al.; Comparison of Cultivations of CHO Cell Line in Chemostat and Acceleratostat Experiments, R.B. Nielsen et al.; Evaluation of a Novel Micro-bioreactor System for Cell Culture Optimisation, G. Wilson.; Comparison of Different Design Configurations of a 1-L Multi-bioreactor Station for Cell Culture Process Development, H. Schütte et al.; The Effect of Genetically Modified Insulin Growth Factor on Cell Proliferation and Adenovirus Productivity in Serum Free Medium, A. Buckler et al.; Evaluation of a Scalable Disposable Bioreactor System for Manufacturing of Mammalian Cell Based Biopharmaceuticals, S. Langhammer et al.; Purification of Rabies Virus Produced on Vero Cells Using Chromatography Techniques, M. Chtioui et al.; Human Growth Hormone (HGH) Purification From CHO-Cell Culture Supernatant Utilizing Macroporous Chromatographic Media, J.-G. Walter et al.; Process Development for a Veterinary Vaccine Against Heartwater Using Stirred Tanks, I. Marcelino et al.; Working Towards a Chemically-defined Replacement for Hydrolysates, Z.W. Deeds et al.; Effect of Vitamins or Lipids Addition on Adenovirus Production at High Cell Densities, T.B. Ferreira et al.; Observation and Analysis of Lab Scaled Microcarrier Cultivation by In-situ Microscopy with Image Processing Tools, G. Rudolph et al.; Rapeseed Peptidic Fractions as Medium Additives in Animal Cell Bioreactors, B. Farges et al.; Influence of Rapeseed Protein Hydrolysis Conditions on Animal Cell Growth in Serum-free Media Supplemented with Hydrolysates, G. Chabanon et al.; Rapid Media and Process Optimization Using DOE: A Case Study, S. Holdread et al.; Developing Chemically Defined Media Through DOE: Complete Optimization with Increased Protein Production in Less than 8 Months, K. Hammett et al.; Bioreactor Process Strategies for rNS0 Cells, D. Fernandez et al.; The Efficient Induction of Human Antibody Production in in Vitro Immunization by CPG Oilgodeoxynucleotides, Y. Aiba et al.; Rapid Culture Media Optimization to Gain Multiple Fold Increase in Recombinant Protein Production in Two CHO Cell Lines, S.D. Storms et al.; Strategies for Large Scale Production of Parapoxvirus Ovis NZ-2, M. Pohlscheidt et al.; Proteomic Investigation of Recombinant CHO Cells in High Density Culture, J.R. Cresswell et al.; Comparative Proteomic Study of Mechanisms Involved in the Expression of Recombinant Monoclonal Antibody in Non Amplified NS0 Myeloma Cell Line, L.E. Rojas et al.; Monitoring Nutrient Limitations by Online Capacitance Measurements in Batch & Fed-batch CHO Fermentations, S. Ansorge et al.; Characterisation of Shake Flasks for Cultivation of Animal Cell Cultures, G. Jänicke et al.; Manufacturing of Lots of Human T-Lymphocytes for Cell Therapy, N.D. Bernardi et al.; Oxygen Control in Static Cell Cultures, N.D. Bernardi et al.; The Effects of Cell Separation with Hydrocyclones on the Viability of CHO Cells, R.C.V. Pinto et al.; Erythropoietin Purification Employing Affinity Membrane Adsorbers, M.C.M. Mellado et al.; Cultivation of Vero Cells on Microporous and Macroporous Microcarriers, M.C.O. Souza et al.; CFD-Aided Design of Hollow Fibre Modules for Integrated Mammalian Cell Retention and Product Purification, R.L. Machado et al.; Comparison of Manufacture Technologies for RAV12 Monoclonal Antibody in Production Medium Containing No Animal Derived Proteins, I. Shackel et al.; Highly Efficient Serum-free Production of Biopharmaceuticals in CHO-Cells, S. Zahn et al.; A New Host Cell-line for the Fast, Reproducible and Efficient Production of Biopharmaceuticals, B. Greulich et al.; Proteomic Characterization of Recombinant NS0 Cell Clones Obtained by an Integrated Selection Strategy, A. Castillo et al.; Analysis and Modeling of the Metabolism of Lepidopteran Cell Lines, J.-C. Drugmand et al.; Growth of Mammalian and Lepidopteran Cells on BioNOC® II Disks, a Novel Macroporous Microcarrier, J.-C. Drugmand et al.; Screening Strategies for Iron Chelators in Serum Free Media, J. Keenan et al.; Production of Human Growth Hormone in a Mammalian Cell High Density Perfusion Process, T. Nottorf et al.; Development of an Integrated Qualitative and Quantitative Approach to Study Expression-Secretion Bottlenecks of Mammalian cDNAs in HEK293 EBNA Cells, L. Glez et al.; Rotating Bed Bioreactors: a Novel Way to Natural Proteins by Tissue-like Cultivation of Mammalian Cells, H.P.A. Hoffmeister et al.; Minireactors for Animal Cell Culture: Monitoring of Cell Concentration and Cellular Activity, A. Soley et al.; Air-lift Bioreactor Improvement of Scale-up Linearity Between Shake Flasks and 25-L Scale in the Insect Cells–baculovirus Expression System, F. Bollin et al.; Name Index; Subject Index.

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