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Cellular and Molecular Procedures in Developmental Biology

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This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures.

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* Provides straightforward, current ...

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1998 Hardcover Very good 8vo, hardcover. No dj, as issued. Vg condition. Non-circulating ex-lib copy w/ label on endpaper, cover label neatly removed (only markings). Contents ... quite bright & clean, binding tight; covers glossy. 329 pp. Read more Show Less

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Overview

This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures.

Key Features
* Provides straightforward, current protocols and critical appraisals
* Includes diverse analysis of cellular and molecular techniques
* Presents everything from whole-organ cultures to electrophysiological approaches
* Details a variety of methods for interfering with gene function in various species
* Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis

Audience: Graduate students and researchers in developmental, molecular, and cell biology, as well as genetics, zoology, agriculture, and neuroscience.

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Editorial Reviews

Leo Miller
This book, part of the "Current Topics in Developmental Biology" series, summarizes various techniques currently used in developmental biology, including production of chicken-quail chimeras, procedures for interfering with the expression of genes, cloning, in vitro culture models for the analysis of nervous system cells and tissues, and electrophysiological techniques. The purpose is to provide a description of procedures that have recently become popular or techniques that have not been reviewed recently in other texts. Special emphasis is put on procedures used by developmental neurobiologists. This book is aimed at developmental biologists doing research in vertebrate or invertebrate developmental biology, especially those interested in neurobiology. The different chapters have a nice balance of illustrations. The color photographs are especially helpful and references are, in general, up-to-date. Like most volumes of current topics in developmental biology, this one is quite diverse. It includes techniques for chick, frog, Drosophila, and mouse embryos. In addition, there are chapters on procedures such as flow cytometry, in situ hybridization, apoptosis, cell cycle biology, and electrophysiology. All of the chapters are well done and will serve as good reviews of the topics. This book is highly recommended.
From The Critics
Reviewer:Leo Miller, PhD (University of Illinois at Chicago)
Description:This book, part of the "Current Topics in Developmental Biology" series, summarizes various techniques currently used in developmental biology, including production of chicken-quail chimeras, procedures for interfering with the expression of genes, cloning, in vitro culture models for the analysis of nervous system cells and tissues, and electrophysiological techniques.
Purpose:The purpose is to provide a description of procedures that have recently become popular or techniques that have not been reviewed recently in other texts. Special emphasis is put on procedures used by developmental neurobiologists.
Audience:This book is aimed at developmental biologists doing research in vertebrate or invertebrate developmental biology, especially those interested in neurobiology.
Features:The different chapters have a nice balance of illustrations. The color photographs are especially helpful and references are, in general, up-to-date.
Assessment:Like most volumes of current topics in developmental biology, this one is quite diverse. It includes techniques for chick, frog, Drosophila, and mouse embryos. In addition, there are chapters on procedures such as flow cytometry, in situ hybridization, apoptosis, cell cycle biology, and electrophysiology. All of the chapters are well done and will serve as good reviews of the topics. This book is highly recommended.
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Product Details

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The Longest-Running Public Forum for Contemporary Issues in Developmental Biology
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Table of Contents

E. Dupin, C. Ziller, and N.M. Le Douarin, The Avian Embryo as a Model in Developmental Studies.
A.V. Morales and F. de Pablo, Inhibition of Gene Expression by Antisense Oligonucleotides in Chick Embryos in Vitro and in Vivo.
C.L. Cepko, E. Ryder, C. Austin, J. Golden, and S. Fields-Berry, Lineage Analysis Using Retroviral Vectors.
G. Lagna and A. Hemmati-Brivanlou, Use of Dominant/Negative Constructs to Modulate Gene Expression.
M. Torres, The Use of Embryonic Stem Cells for the Genetic Manipulation of the Mouse.
J.J. Garrido, T. Schimmang, J. Represa, and F. Giraldez, Organoculture of Otic Vesicle and Ganglion.
E.J. de la Rosa, B. Diaz, and F. de Pablo, Organoculture of the Chick Embryonic Neuroretina.
C.E. Krull and P.M. Kulesa, Embryonic Explant and Slice Preparations for Studies of Cell Migration and Axon Guidance.
A.V. Holst and H. Roher, Culture of Avian Sympathetic Neurons.
M-J. Fann and P.H. Patterson, Analysis of Gene Expression in Cultured Primary Neurons.
S. Nakagawa, H. Matsunami, and M. Takeichi, Selective Aggregation Assays for Embryonic Brain Cell and Cell Lines.
J. Serna, B. Pimentel, and E.J. de la Rosa, Flow Cytometric Analysis of Whole Organs and Embryos.
C.D. Stern, Detection of Multiple Gene Products Simultaneously by in Situ Hybridization and Immunohistochemistry in Whole Mounts of Avian Embryos.
C. Dulac, Differential Cloning from Single Cell cDNA Libraries.
F. Feiguin, S. Llamazares, and C. Gonzalez, Methods in Drosophila Cell Cycle Biology.
J. Lerma, M. Morales, and M. de los Angeles Vincente, Single CNS Neurons in Culture.
M. Martinez-Padron and A. Ferrus, Patch-Clamp Recordings from Drosophila Presynaptic Terminals.
N.J. McCarthy and G.I. Evan, Methods for Detecting and Quantifying Apoptosis.
Subject Index.
Contents of Previous Volumes.
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