Cellular and Molecular Procedures in Developmental Biologyby Roger A. Pedersen
Cellular and Molecular Procedures in Developmental Biology addresses a number of key advances in cell and developmental biology research. This volume incorporates practical methods at the level of molecular, cellular, and whole-organism biology in vertebrate and invertebrate models. It will not only be of interest to the seasoned professional, but will also/b>… See more details below
Cellular and Molecular Procedures in Developmental Biology addresses a number of key advances in cell and developmental biology research. This volume incorporates practical methods at the level of molecular, cellular, and whole-organism biology in vertebrate and invertebrate models. It will not only be of interest to the seasoned professional, but will also serve as an excellent tutorial for the interested novice.
• Provides straightforward, current protocols and critical appraisals
• Includes diverse analysis of cellular and molecular techniques
• Presents everything from whole-organ cultures to electrophysiological approaches
• Details a variety of methods for interfering with gene function in various species
• Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis
Description:This book, part of the "Current Topics in Developmental Biology" series, summarizes various techniques currently used in developmental biology, including production of chicken-quail chimeras, procedures for interfering with the expression of genes, cloning, in vitro culture models for the analysis of nervous system cells and tissues, and electrophysiological techniques.
Purpose:The purpose is to provide a description of procedures that have recently become popular or techniques that have not been reviewed recently in other texts. Special emphasis is put on procedures used by developmental neurobiologists.
Audience:This book is aimed at developmental biologists doing research in vertebrate or invertebrate developmental biology, especially those interested in neurobiology.
Features:The different chapters have a nice balance of illustrations. The color photographs are especially helpful and references are, in general, up-to-date.
Assessment:Like most volumes of current topics in developmental biology, this one is quite diverse. It includes techniques for chick, frog, Drosophila, and mouse embryos. In addition, there are chapters on procedures such as flow cytometry, in situ hybridization, apoptosis, cell cycle biology, and electrophysiology. All of the chapters are well done and will serve as good reviews of the topics. This book is highly recommended.
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