Overview

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.




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Cilia, Part A

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Overview

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.




  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers cilia
  • Contains chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium
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Product Details

  • ISBN-13: 9780123984869
  • Publisher: Elsevier Science
  • Publication date: 3/12/2013
  • Sold by: Barnes & Noble
  • Format: eBook
  • Pages: 472
  • File size: 5 MB

Meet the Author

Wallace Marshall is an electrical engineer by training, who became interested in biology out of a desire to understand how cells solve engineering problems, such as determining the size of organelles. He received his Ph.D. at UCSF with John Sedat, where he studied the diffusional of motion of interphase chromatin using live cell imaging and computational image analysis. He then trained as a postdoc with Joel Rosenbaum at Yale, where he began studying the mechanisms regulating the length of cilia and flagella. He is now Profess of Biochemistry at UCSF, where he lab continues to study the assembly and length regulation of cilia and flagella, as well as the mechanisms that regulate the size of other organelles. His work takes advantage of an integrated combination of methods including genetics, microscopy, and computational modeling, as well as a wide variety of model organisms including Chlamydomonas reinhardtii, Stentor coeruleus, yeast, flatworms, and mammalian cells.

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Table of Contents


  1. Electron microscopy of IFT in cilia and flagella
  2. Biochemical analysis of ciliary transport
  3. Visualization of IFT in Chlamydomonas flagella
  4. Visualization of kidney cilia-mediated calcium signaling
  5. Methods for analyzing nodal cilia and flow
  6. Methods for building synthetic cilia-like motor-microtubule arrays
  7. Isolation of IFT particle proteins from Chlamydomonas
  8. Radial Spoke isolation and assays
  9. Biomechanical measurements of kinocilium
  10. Methods for analysis of Calcium/calmodulin signaling in flagella
  11. Ubiquitination of Flagellar Proteins
  12. Visualizing IFT in C. elegans cilia
  13. Proteomic analysis of mammalian primary cilia
  14. TSC and TOR signaling in cilia
  15. Bioinformatic approaches to cilia
  16. Methods for testing cilia function in cell migration
  17. Signaling and waveform in Drosophila sperm flagella
  18. Analysis of import into primary cilia
  19. Patch-Clamp Recording from Mouse and Human Spermatozoa
  20. Optical trap measurements of flagellar membrane motility
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