Clinical and Forensic Applications of Capillary Electrophoresis / Edition 1

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Overview

John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and non-aqueous electrophoresis. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.

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Editorial Reviews

Doody's Review Service
Reviewer: Valerie L. Ng, PhD MD(Alameda County Medical Center/Highland Hospital)
Description: This is a comprehensive review of the principles and current and future clinical applications of capillary electrophoresis.
Purpose: The intent is to give the reader an overview of the many clinical and forensic uses of capillary electrophoresis.
Audience: This book would appeal to a wide range of readers, including those interested in learning more about the basic principles, those interested in detailed discussion about its current use, and those who are interested in the visionary future uses of capillary electrophoresis.
Features: The 20 chapters are divided into six major groups: anintroduction and basic principles, protein electrophoresis, metabolic diseases, immunoassay, molecular diagnostics, and mass spectrometry/therapeutic drug monitoring/toxicology. The introductory section gives an excellent and concise summary of the principles and current clinical uses of capillary electrophoresis. The remaining chapters are progressively less satisfying proportional to the remoteness (i.e., in actual practice versus purely speculative) of the application of capillary electrophoresis to its actual use. For example, the sections on immunoassays and molecular diagnostics simply speculate on potential applications of capillary electrophoresis to these disciplines, solely related to its proven ability of analyte separation. These speculations are followed by a discussion of the problem of serial and single sampling inherent to capillary electrophoresis, thereby negating the previous discussion of its advantages! The chapters are written in very individual styles with little sense of cohesiveness, leaving one with a sense of reading a disparate set of treatises linked only by a common technology. The few illustrations are in black and white and relatively unrefined.
Assessment: This book fills a niche for those curious about this technology. It would be of greatest use to those with an academic interest in capillary electrophoresis. The average user would be hard pressed to justify this purchase given that a significant portion of the book is given to speculation regarding the future application of this technology.
Booknews
Overviews the current use of capillary electrophoresis (CE) in the clinical laboratory as a separation method for proteins, metabolic diseases, and immunoassay. The 20 chapters review the coating of columns, the analysis of serum and urine for proteins, abnormal hemoglobins and hemoglobin Alc, peptides, amino and organic acids, viral load, and short tandem repeats. The methods include capillary zone, micellar, electrokinetic, capillary gel, and non-aqueous electrophoresis. The last section describes how CE can be used in conjunction with mass spectroscopy to detect heavy metal poisoning, screen for drugs of forensic significance, and monitor therapeutic drug levels. Annotation c. Book News, Inc., Portland, OR (booknews.com)

2 Stars from Doody
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Product Details

  • ISBN-13: 9780896036451
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 4/20/2001
  • Series: Pathology and Laboratory Medicine Series
  • Edition description: 2001
  • Edition number: 1
  • Pages: 454
  • Product dimensions: 9.21 (w) x 6.14 (h) x 1.00 (d)

Table of Contents

Preface
List of Contributors
Sect. I Introduction 1
1 Capillary Electrophoresis: General Overview and Applications in the Clinical Laboratory 3
2 Basic Principles and Modes of Capillary Electrophoresis 21
Sect. II Protein Electrophoresis 59
3 Capillary Coatings for Protein Analysis 61
4 Clinical Serum Protein Capillary Zone Electrophoresis 79
5 Serum and Urine Paraprotein Capillary Electrophoresis 93
6 Cerebrospinal Fluid Protein Electrophoresis 105
7 Lipoprotein Analysis 113
8 Clinical Analysis of Structural Hemoglobin Variants and Hb A[subscript 1c] by Capillary Isoelectric Focusing 145
Sect. III Metabolic Diseases 145
9 Amino Acid Analysis 167
10 Organic Acids 193
11 Steroids 209
Sect. IV Immunoassay 221
12 Capillary Electrophoresis Based Immunoassay 223
Sect. V Molecular Diagnostics 235
13 Quantitation of Viral Load 237
14 The Application of Capillary Electrophoresis in the Analysis of PCR Products Used in Forensic DNA Typing 261
15 Combining Capillary Electrophoresis with Electrospray Ionization Mass Spectrometry 285
Sect. VI Mass Spectrometry, Therapeutic Drug Monitoring, and Toxicology 315
16 Capillary Electrophoresis-Mass Spectrometry of Biologically Active Peptides and Proteins 317
17 Serum Drug Monitoring by Capillary Electrophoresis 355
18 Applications of Capillary Zone Electrophoresis in the Analysis of Metal Ions of Clinical Significance 385
19 Clinical and Forensic Drug Toxicology: Analysis of Illicit and Abused Drugs in Urine by Capillary Electrophoresis 397
20 Screening Biological Specimens for Drugs of Forensic Significance 423
Index 437
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