Confocal, Multiphoton, and Nonlinear Microscopic Imaging

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Table of Contents

Conference Committee
Symposium Committee
Multiphoton imaging using a Ti:sapphire regenerative amplifier 1
Compensation of temporal and spatial dispersion for multiphoton acousto-optic laser-scanning microscopy 7
Fluorescence enhancement near metallic nanoparticies under two-photon excitation 20
New two-photon excitation chromophores for cellular imaging 27
A novel diode-pumped tunable system for multiphoton microscopy 36
Multiphoton microscopy at an excitation wavelength of 1.26 [mu]m 42
Multiphoton imaging in the detection of malignancy in the oesophagus 51
Ultrasensitivity, speed, and resolution: optimizing low-light microscopy with the back-illuminated electron-multiplying CCD 56
Three-dimensional imaging and quantification of complex vascular networks 67
Confocal microscopy in transmitted light 79
Use of acceptor fluorescence for determining FRET lifetimes 88
Confocal microscopy: an experimental set up for biomolecule structure investigation based on dynamical fluorescence spectroscopy
Photo-induced flip-flop of membrane markers monitored by SHG microscopy 107
Second harmonic generation imaging in muscle fibers 112
Chiral chromophores for second harmonic microscopy 121
CARS microscopy with dark field excitation 129
GRISM-based probe for spectrally encoded confocal microscopy 139
Advances in multispectral confocal imaging 147
Fluorescence lifetime imaging (FLIM) of membrane markers in living cells 158
Time-resolved microspectrofluorometry and fluorescence lifetime imaging using ps-pulsed diode lasers in laser scanning microscopes 166
Time-resolved multifocal multiphoton microscopy 173
Imaging protein-protein interactions by multiphoton FLIM 180
Analysis of the 3D structure of agglutinated erythrocyte using CellScan and confocal microscopy: characterization by FLIM-FRET 190
Comparison of human colorectal normal tissue with cancerous tissue autofluorescence image by optical sectioning with a confocal laser-scanning microscope 199
Speckle microscopy for blood flow measurements in the smallest microvessel 206
Time-resolved multiphoton imaging of the interaction between the PKC and the NF[kappa]B signalling pathways 216
Novel efficient and stable heteroaromatic two-photon absorbing dyes 223
Simultaneous (almost) imaging of intracellular Ca[superscript 2+] and Na[superscript +] in sea-urchin oocyte by using a tunable liquid crystal filter 231
Linked-fluorophore FRET calibration and FRET studies of the cyclin-CDK switch in mammalian cells 238
Development of a low-cost SOM laboratory prototype with line illumination: evaluation of deconvolution techniques on its axial resolution 245
Author Index 253
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