Contemporary Enzyme Kinetics and Mechanism: Selected Methods in Enzymology [NOOK Book]

Overview

Contemporary Enzyme Kinetics and Mechanism

Audience: Students and professionals in biochemistry, biophysics, molecular biology, pharmacology, and pharmaceutical chemistry.

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Contemporary Enzyme Kinetics and Mechanism: Selected Methods in Enzymology

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Overview

Contemporary Enzyme Kinetics and Mechanism

Audience: Students and professionals in biochemistry, biophysics, molecular biology, pharmacology, and pharmaceutical chemistry.

Read More Show Less

Editorial Reviews

Gene A. Homandberg
This multiauthored second edition monograph is, as was the first edition published in 1983, a compilation of basic articles from the Enzymes and Kinetics series of Methods in Enzymology. The chapters were written by 19 contributors from various specialties. Most of the chapters have been updated, and 10 of the 17 chapters are new. "This monograph is meant to update the earlier volume to continue to provide assistance in design, execution and analysis of enzyme systems. Since the first edition in 1983, the dramatic growth of this area has necessitated a newer edition. For example, this edition has been updated extensively and, for example, now includes a very interesting chapter on site-directed mutagenesis. "Although the audience is meant to be biochemists and biophysicists, this monograph is an ideal primary textbook for students in this area. "This softcover book features the typical attractive Methods in Enzymology format, with a suitable number of black-and-white illustrations. The book also features a chapter on selected exercises and problems of both a quantitative and descriptive nature. "This book is highly recommended because it contains basic information and discussion that covers earlier work to more recent work on enzyme dynamics. It is also very important because of the increasing need for more nonspecialists to be able to study newly discovered or expressed enzymes.
From The Critics
Reviewer:Gene A. Homandberg, PhD(Rush Medical College of Rush University)
Description:This multiauthored second edition monograph is, as was the first edition published in 1983, a compilation of basic articles from the Enzymes and Kinetics series of Methods in Enzymology. The chapters were written by 19 contributors from various specialties. Most of the chapters have been updated, and 10 of the 17 chapters are new.
Purpose:This monograph is meant to update the earlier volume to continue to provide assistance in design, execution and analysis of enzyme systems. Since the first edition in 1983, the dramatic growth of this area has necessitated a newer edition. For example, this edition has been updated extensively and, for example, now includes a very interesting chapter on site-directed mutagenesis.
Audience:Although the audience is meant to be biochemists and biophysicists, this monograph is an ideal primary textbook for students in this area.
Features:This softcover book features the typical attractive Methods in Enzymology format, with a suitable number of black-and-white illustrations. The book also features a chapter on selected exercises and problems of both a quantitative and descriptive nature.
Assessment:This book is highly recommended because it contains basic information and discussion that covers earlier work to more recent work on enzyme dynamics. It is also very important because of the increasing need for more nonspecialists to be able to study newly discovered or expressed enzymes.
Booknews
A collection of articles from vols. 63, 64, 87, and 249 of Methods in Enzymology, describing widely used strategies in kinetic studies of enzyme action and offering exercises and problems. Subjects include cooperativity in enzyme function, product inhibition applications, mechanism-based enzyme inactivators, and hydrogen tunneling in enzyme catalysis. Includes the complete table of contents for each of the volumes used in the collection. This second edition contains articles from vol. 249, and ten of the 17 chapters are new. Annotation c. Book News, Inc., Portland, OR (booknews.com)

3 Stars from Doody
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Product Details

  • ISBN-13: 9781483214221
  • Publisher: Elsevier Science
  • Publication date: 1/1/1983
  • Sold by: Barnes & Noble
  • Format: eBook
  • Pages: 566
  • File size: 9 MB

Meet the Author

Daniel Lee Purich has been at the forefront of biochemistry research for more than 25 years. He was awarded the National Institutes of Health Research Career Development Award from 1977-1982, the Plous Teaching Award (the University of California Santa Barbara Campus-Wide Teaching Award) in 1977, has been a member of the National Institutes of Health Biochemistry Study Section from 1982-1985, and a member of the Journal of Biological Chemistry Editorial Board from 1981-1986. He has been a member of the American Society of Biological Chemists, the American Chemical Society, the New York Academy of Sciences, the Biochemical Society, and the American Society for Cell Biology. Dr. Purich is currently a Professor and Chairman of the Department of Biochemistry and Molecular Biology at the Florida College of Medicine. He is the author and editor of numerous scientific publications.

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Table of Contents

List of Contributors
Preface
Contents of Methods in Enzymology, Volumes 63, 64, 87, and 249
1 Transient Kinetic Approaches to Enzyme Mechanisms 1
2 Practical Considerations in the Design of Initial Velocity Enzyme Rate Assays 37
3 Derivation of Initial Velocity and Isotope Exchange Rate Equations 57
4 Plotting Methods for Analyzing Enzyme Rate Data 89
5 Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination between Rival Kinetic Models 111
6 Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects 133
7 Product Inhibition Applications 183
8 Reversible Enzyme Inhibitors as Mechanistic Probes 207
9 Transition State and Multisubstrate Analog Inhibitors 229
10 Application of Affinity Labeling for Studying Structure and Function of Enzymes 259
11 Mechanism Based Enzyme Inactivators 291
12 Isotope Exchange Methods for Elucidating Enzymic Catalysis 335
13 Positional Isotope Exchange as Probe of Enzyme Action 379
14 Isotope Effects: Determination of Enzyme Transition State Structure 407
15 Hydrogen Tunneling in Enzyme Catalysis 441
16 Site-Directed Mutagenesis: A Tool for Studying Enzyme Catalysis 467
17 Selected Exercises and Problems 497
Index 531
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