Culture of Animal Cells: A Manual of Basic Technique

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The most complete resource on the techniques, equipment,principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential...

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More About This Book

Overview

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:

* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols

* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering

* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment

* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text

* Fully updated references

* The clearest, most consistent presentation of step-by-step protocols available

* Numerous diagrams, photographs, tables, and charts

* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites

Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

Appropriate for: Cell Biologists, Cytologists.

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Editorial Reviews

From The Critics
Reviewer: Alvin Telser, PhD(Northwestern University Feinberg School of Medicine)
Description: This is a book on the techniques, equipment, practices, and principles needed to place animal cells into tissue culture. This volume is in its fourth edition.
Purpose: The book is intended to provide a complete technical guide to the growth of animal cells in tissue culture. Like other high quality techniques books, these are worthy objectives and are met admirably.
Audience: According to the author, this book is written for persons with no previous experience with these techniques. It is extremely well written for such an audience. The author is a very well recognized expert in the field.
Features: The full range of information pertinent to the growth of animal cells in tissue culture is covered such as lab layout, necessary equipment, water quality, selection of media and growth chambers, steps starting with whole animal material, observing and staining cultured cells, common contaminants, and more. It is well illustrated. The black-and-white diagrams are very well done and helpful; many of the black-and-white photographs are also very good, but some are of moderate quality, especially many photomicrographs of cells. The newly included center section of color photos is likely to be of marginal value—partly due to low quality original material and partly due to poor quality reproduction.
Assessment: This will be a very helpful reference in any lab actively using tissue culture. It would also be a very useful reference volume in any life science library. The techniques used in tissue culture, like most fields in modern biology, changes fairly frequently, making a new edition every few years important. This book is well worth its cost.
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Product Details

  • ISBN-13: 9780845142417
  • Publisher: Liss, Alan R. Inc.
  • Publication date: 1/1/1987
  • Edition number: 2
  • Pages: 397

Meet the Author

R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.

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Table of Contents

Preface to the Third Edition
Preface to the Second Edition
Preface to the First Edition
Abbreviations
1 Introduction
2 Biology of the Cultured Cell
3 Design and Layout of the Laboratory
4 Equipping the Laboratory
5 Aseptic Technique
6 Laboratory Safety and Biohazards
7 The Culture Environment: Substrate, Gas Phase, Medium and Temperature
8 Preparation and Sterilization
9 Disaggregation of the Tissue and Primary Culture
10 Maintenance of the Culture: Cell Lines
11 Cloning and Selection of Specific Cell Types
12 Physical Methods of Cell Separation
13 Characterization
14 Induction of Differentiation
15 The Transformed Phenotype
16 Contamination
17 Instability, Variation, and Preservation
18 Quantitation and Experimental Design
19 Measurement of Viability and Cytotoxicity
20 Culture of Specific Cell Types
21 Culture of Tumor Tissue
22 Three-Dimensional Culture Systems
23 Specialized Techniques
Reagent Appendix
Trade Index
Glossary
References
Index
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