Culture of Animal Cells: A Manual of Basic Technique / Edition 4

Culture of Animal Cells: A Manual of Basic Technique / Edition 4

by R. Ian Freshney
     
 

Provides an accessible, comprehensive synthesis of proven techniques for the culture and experimental manipulation of animal cells. Contains step-by-step laboratory procedures, detailed instructions for reagent preparation and information on the latest specialized equipment and supplies. This edition includes over 350 new references, 13 new protocols plus recent… See more details below

Overview

Provides an accessible, comprehensive synthesis of proven techniques for the culture and experimental manipulation of animal cells. Contains step-by-step laboratory procedures, detailed instructions for reagent preparation and information on the latest specialized equipment and supplies. This edition includes over 350 new references, 13 new protocols plus recent methods and expanded protocols in areas of new technology such as antibody staining and cytotoxicity assay.

Product Details

ISBN-13:
9780471348894
Publisher:
Wiley
Publication date:
03/01/1900
Edition description:
Older Edition
Pages:
624
Product dimensions:
8.70(w) x 11.28(h) x 1.40(d)

Meet the Author

R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.

Table of Contents

Preface to the Third Edition
Preface to the Second Edition
Preface to the First Edition
Abbreviations
1Introduction
2Biology of the Cultured Cell
3Design and Layout of the Laboratory
4Equipping the Laboratory
5Aseptic Technique
6Laboratory Safety and Biohazards
7The Culture Environment: Substrate, Gas Phase, Medium and Temperature
8Preparation and Sterilization
9Disaggregation of the Tissue and Primary Culture
10Maintenance of the Culture: Cell Lines
11Cloning and Selection of Specific Cell Types
12Physical Methods of Cell Separation
13Characterization
14Induction of Differentiation
15The Transformed Phenotype
16Contamination
17Instability, Variation, and Preservation
18Quantitation and Experimental Design
19Measurement of Viability and Cytotoxicity
20Culture of Specific Cell Types
21Culture of Tumor Tissue
22Three-Dimensional Culture Systems
23Specialized Techniques
Reagent Appendix
Trade Index
Glossary
References
Index

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