Culture of Animal Cells: A Manual of Basic Technique / Edition 4

Culture of Animal Cells: A Manual of Basic Technique / Edition 4

by R. Ian Freshney
     
 

ISBN-10: 0471348899

ISBN-13: 9780471348894

Pub. Date: 03/01/1900

Publisher: Wiley

Provides an accessible, comprehensive synthesis of proven techniques for the culture and experimental manipulation of animal cells. Contains step-by-step laboratory procedures, detailed instructions for reagent preparation and information on the latest specialized equipment and supplies. This edition includes over 350 new references, 13 new protocols plus recent

Overview

Provides an accessible, comprehensive synthesis of proven techniques for the culture and experimental manipulation of animal cells. Contains step-by-step laboratory procedures, detailed instructions for reagent preparation and information on the latest specialized equipment and supplies. This edition includes over 350 new references, 13 new protocols plus recent methods and expanded protocols in areas of new technology such as antibody staining and cytotoxicity assay.

Product Details

ISBN-13:
9780471348894
Publisher:
Wiley
Publication date:
03/01/1900
Edition description:
Older Edition
Pages:
624
Product dimensions:
8.70(w) x 11.28(h) x 1.40(d)

Table of Contents

Biology of the Cultured Cell
Design and Layout of the Laboratory
Equipping the Laboratory
Aseptic Technique
Laboratory Safety and Biohazards
The Culture Environment: Substrate, Gas Phase, Medium and Temperature
Preparation and Sterilization
Disaggregation of the Tissue and Primary Culture
Maintenance of the Culture: Cell Lines
Cloning and Selection of Specific Cell Types
Physical Methods of Cell Separation
Characterization
Induction of Differentiation
The Transformed Phenotype
Contamination
Instability, Variation, and Preservation
Quantitation and Experimental Design
Measurement of Viability and Cytotoxicity
Culture of Specific Cell Types
Culture of Tumor Tissue
Three-Dimensional Culture Sytems
Specialized Techniques
Reagent Appendix
Glossary
References
Indexes

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