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Doody's Review ServiceReviewer: Alvin Telser, PhD (Northwestern University Feinberg School of Medicine)
Description: This is a book on the techniques, equipment, practices, and principles needed to place animal cells into tissue culture. This volume is in its fourth edition.
Purpose: The book is intended to provide a complete technical guide to the growth of animal cells in tissue culture. Like other high quality techniques books, these are worthy objectives and are met admirably.
Audience: According to the author, this book is written for persons with no previous experience with these techniques. It is extremely well written for such an audience. The author is a very well recognized expert in the field.
Features: The full range of information pertinent to the growth of animal cells in tissue culture is covered such as lab layout, necessary equipment, water quality, selection of media and growth chambers, steps starting with whole animal material, observing and staining cultured cells, common contaminants, and more. It is well illustrated. The black-and-white diagrams are very well done and helpful; many of the black-and-white photographs are also very good, but some are of moderate quality, especially many photomicrographs of cells. The newly included center section of color photos is likely to be of marginal value—partly due to low quality original material and partly due to poor quality reproduction.
Assessment: This will be a very helpful reference in any lab actively using tissue culture. It would also be a very useful reference volume in any life science library. The techniques used in tissue culture, like most fields in modern biology, changes fairly frequently, making a new edition every few years important. This book is well worth its cost.