Culture of Epithelial Cells / Edition 2

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"It is now the age of the specialized cell in culture," R. Ian Freshney wrote in the preface to the first edition of Culture of Epithelial Cells. Realizing the inadequacy of studying all types of cells at random in a culture, it became imperative for researchers to learn how unique cells perform under a wide variety of conditions. Now the Second Edition of Culture of Epithelial Cellsdevoted to those cells that line the body, internally and externally, protecting surfaces and secreting or absorbing materialadvances this ethic, providing completely revised, detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia.Each chapter of the Second Edition is devoted to a different type of epitheliumepidermal, mammary, cervical, prostatic, oral, intestinal, pulmonary, hepatic, and urothelial. R. Ian Freshney also contributes a final chapter on a number of epithelia not covered in the main chapters, presenting them together in review form with additional protocols. Other features of the Second Edition of Culture of Epithelial Cells include:Proven methods refined by the authors, all well-established researchers Alternate techniques coupled with critical comparative analysesLogical anatomical organization by tissue typeStepwise culture protocols conveniently highlightedNew chapters on the culture of oral, alveolar, intestinal, and urothelial epitheliumIn-depth analysis of cell-cell interaction in skin Displaying the clear, easy-to-follow style that has become a trademark of the Culture of Specialized Cells series, the Second Edition of Culture of Epithelial Cells remains an essential resource for researchers in cell and developmental biology, cancer biology, tissue engineering, biochemistry, and genetics, where the work requires the cultivation or manipulation of cells from the epithelium.

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Editorial Reviews

From the Publisher
"...a wonderful compendium of current in vitro approachesthat will be a useful resource to those just starting to work withan epithelial cell system as well as those that have been workingwith them for years and years." (Pharmaceutical Research,Vol. 19, No. 10, October 2002)

• a wonderful compendium of current in vitro approaches that willbe a useful resource to those just starting to work with anepithelial cell system as well as those that have been working withthem for years and years.? (Pharmaceutical Research , Vol. 19, No.10, October 2002)

This first in a planned series of books on the culture and manipulation of specialized cells fully describes procedures currently used for the in vitro cultivation of various types of epithelia. Designed as a practical sourcebook for laboratory practitioners, it presents proven methods that have been refined by established investigators, accompanied by discussions of the culture environment conditions most suitable for the survival and growth of particular cells of interest. Annotation c. Book News, Inc., Portland, OR (
From The Critics
Describes some of the more commonly studied epithelia, and presents specific protocols for their cultivation and characterization. The 13 chapters provide detailed instructions for the preparation and culture of human epidermal, mammary, cervical, prostatic, and bronchial epithelial cells. The second edition adds chapters on oral, alveolar, intestinal, and urothelial epithelium. Annotation c. Book News, Inc., Portland, OR (
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Product Details

  • ISBN-13: 9780471401216
  • Publisher: Wiley
  • Publication date: 4/28/2002
  • Series: Culture of Specialized Cells Series, #6
  • Edition description: REV
  • Edition number: 2
  • Pages: 461
  • Product dimensions: 7.05 (w) x 10.04 (h) x 0.93 (d)

Table of Contents


Preface to 2nd Edition (R. Freshney).

List of Abbreviations.

Introduction (R. Freshney).

Cell Interaction and Epithelial Differentiation (N. Maas-Szabowski,et al.).

The Epidermis (E. Parkinson & W. Yeudall).

Culture of Human Mammary Epithelial Cells (M. Stampfer, etal.).

Culture of Human Cervical Epithelial Cells (M. Stanley).

Human Prostatic Epithelial Cells (D. Peehl).

Culture of Oral Epithelium (R. Grafström).

Normal Human Bronchial Epithelial Cell Culture (J. Wise & J.Lechner).

Isolation and Culture of Pulmonary Alveolar Epithelial Type IICells (L. Dobbs & R. Gonzalez).

Isolation and Culture of Intestinal Epithelial Cells (C. Booth& J. O'Shea).

Isolation and Culture of Animal and Human Hepatocytes (C.Guguen-Guillouzo).

Culture of Human Urothelium (J. Southgate, et al.).

Other Epithelial Cells (R. Freshney).

List of Suppliers.


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