Culture of Epithelial Cells / Edition 2

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Overview

This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.
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Editorial Reviews

Booknews
This first in a planned series of books on the culture and manipulation of specialized cells fully describes procedures currently used for the in vitro cultivation of various types of epithelia. Designed as a practical sourcebook for laboratory practitioners, it presents proven methods that have been refined by established investigators, accompanied by discussions of the culture environment conditions most suitable for the survival and growth of particular cells of interest. Annotation c. Book News, Inc., Portland, OR (booknews.com)
From The Critics
Describes some of the more commonly studied epithelia, and presents specific protocols for their cultivation and characterization. The 13 chapters provide detailed instructions for the preparation and culture of human epidermal, mammary, cervical, prostatic, and bronchial epithelial cells. The second edition adds chapters on oral, alveolar, intestinal, and urothelial epithelium. Annotation c. Book News, Inc., Portland, OR (booknews.com)
From the Publisher
"...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years." (Pharmaceutical Research, Vol. 19, No. 10, October 2002)

?a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years.? (Pharmaceutical Research , Vol. 19, No. 10, October 2002)

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Product Details

  • ISBN-13: 9780471401216
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 4/28/2002
  • Series: Culture of Specialized Cells Series , #6
  • Edition description: REV
  • Edition number: 2
  • Pages: 461
  • Product dimensions: 7.05 (w) x 10.04 (h) x 0.93 (d)

Table of Contents

Contributors
Preface
Preface to First Edition
List of Abbreviations
Ch. 1 Introduction 1
Ch. 2 Cell Interaction and Epithelial Differentiation 31
Ch. 3 The Epidermis 65
Ch. 4 Culture of Human Mammary Epithelial Cells 95
Ch. 5 Culture of Human Cervical Epithelial Cells 137
Ch. 6 Human Prostatic Epithelial Cells 171
Ch. 7 Human Oral Epithelium 195
Ch. 8 Normal Human Bronchial Epithelial Cell Culture 257
Ch. 9 Isolation and Culture of Pulmonary Alveolar Epithelial Type II Cells 277
Ch. 10 Isolation and Culture of Intestinal Epithelial Cells 303
Ch. 11 Isolation and Culture of Animal and Human Hepatocytes 337
Ch. 12 Culture of Human Urothelium 381
Ch. 13 Other Epithelial Cells 401
List of Suppliers 437
Index 443
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