Culture of Human Stem Cells / Edition 1

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This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource.

This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture.

The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

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Editorial Reviews

Doody's Review Service
Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: Stem cells are present at crossroads along the developmental landscape. In addition to self-renewal, these remarkable cells are able to "interpret" instructions regarding lineage and differentiation. This book details methods for derivation, culture, and differentiation of human stem cells obtained from both embryonic and adult sources. The 13 chapters cover topics ranging from culture of the human germ cell lineage to multipotent stem cells in dental pulp to culturing mammary stem cells. The primary focus of the book is on cell and developmental biology.
Purpose: According to the editors, the aim is to make available a "repertoire of established [assist] the large influx of workers." They write, "This book provides a suitable introductory text that will allow incomers to the become familiar with some of the techniques in current use, to increase their knowledge of the discipline, or to develop their own research program."
Audience: The book is written for basic science and clinical researchers, particularly those interested in current topics in developmental biology and the promise of stem cells. Biomedical scientists and clinicians interested in transplantation medicine will also appreciate the wealth of information and exciting ideas presented here.
Features: Each chapter includes a table of contents, introduction, list of essential materials, overviews for specific protocols, and step-by-step instructions for laboratory techniques. The authors provide examples of results from their laboratories. The chapters are written by experts and the book is carefully edited, while line drawings serve to illustrate conceptual points. Color plates convey the sense of excitement one has when viewing stem cells and their derivatives under the microscope. The book includes lists of abbreviations and laboratory suppliers.
Assessment: The principal value of this book lies in the diversity of topics. In addition to chapters on embryonic stem (ES) cells, interesting chapters cover the derivation of multipotential stem cells from mammary tissue, dental pulp, cornea, cord blood, and adipose tissue. The chapters are written for novices as well as experts. One caveat is that the numerical tags used for paragraph headings and tables are often jumbled. For example, we read an overview labeled "" and simultaneously gaze at Table 3.4 and Table 3.10. Overall, this book is a welcome addition to the literature and the detailed protocols will provide useful tools to stimulate progress in stem cell biology and regenerative medicine.
From the Publisher
"For those working, or considering working with human stem cells, Culture of Human Stem Cells will be an essential reference." (The Biochemical Society, October 2009)

"Valuable to those entering the field from a wide spectrum of disciplines…an essential textbook for teachers and students who are involved with the therapeutic potential of stem cell research." (, October 2007)

"The principal value of this book lies in the diversity of topics. In addition to chapters on embryonic stem (ES) cells, interesting chapters cover the derivation of multipotential stem cells from mammary tissue, dental pulp, cornea, cord blood, and adipose tissue." (Doody's)

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Product Details

  • ISBN-13: 9780470052464
  • Publisher: Wiley
  • Publication date: 6/29/2007
  • Series: Culture of Specialized Cells Series , #12
  • Edition description: New Edition
  • Edition number: 1
  • Pages: 368
  • Product dimensions: 7.34 (w) x 10.02 (h) x 0.90 (d)

Meet the Author

R. Ian Freshney, PhD, Honorary Senior Research Fellow in the Centre for Oncology and Applied Pharmacology at the University of Glasgow, is the bestselling author of Culture of Animal Cells, which appeared in its fifth edition in 2005, and is the Series Editor for the Culture of Specialized Cells series (all published by Wiley).

Glyn N. Stacey, PhD, is Director of the United Kingdom Stem Cell Bank, and Head of the Cell Biology and Imaging Division at the National Institute for Biological Standards and Control. Dr. Stacey is internationally known and a sought-after speaker on safety and quality control issues in the use of stem cells.

Jonathan M. Auerbach, PhD, previously director of the Stem Cell Center, American Type Culture Collection, in Manassas, Virginia, is now a Project Leader with GlobalStem, Inc., in Rockville, Maryland, and has been at the forefront of human stem cell research for many years.

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Read an Excerpt

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Table of Contents


List of Contributors.


1. Quality Control Procedures for Stem Cell Lines (Glyn N. Stacey and Jonathan M. Auerbach).

2. Human Embryonal Stem Cell Lines: Derivation and Culture (Jessica A. Cooke and Stephen L. Minger).

3. Techniques for Neural Differentiation of Human EC and ES Cells (Jamie P Jackson, Peter D Tonge and Peter W Andrews).

4. Cardiomyocyte Differentiation in Human ES Cells (Christine Mummery).

5. Culture of the Human Germ Cell Lineage (Lee Turnpenny and Neil A Hanley).

6. Derivation and Culture of Human Embryonal Carcinoma Stem Cell Lines (Stefan Przyborski).

7. Culture of Umbilical Cord- and Cord Blood-Derived Stem Cells (Young-Jin Kim).

8. Multipotent Stem Cells in Dental Pulp (Wataru Sonoyama, Takayoshi Yamaza, Stan Gronthos, and Songtao Shi).

9. Fundamentals of Culture and Characterization of Mesenchymal Stem/Progenitor Cells (MSCs) from Bone Marrow Stroma (Carl A. Gregory and Darwin J. Prockop).

10. Isolation, Characterization, and Culture of Soft Connective Tissue Stem/Progenitor Cells (Charles W Archer, Sarah Oldfield, Samantha Redman, Laura Haughton, Gary Dowthwaite, Ilyas Khan and Jim Ralphs).

11. Culture of Human Corneal Stem Cells (Yiqin Du and James L. Funderburgh).

12. Culturing Mammary Stem Cells (Mark A. LaBarge, Ole W. Petersen, and Mina J. Bissell).

13. Tissue Culture of Adipose-Derived Stem Cells (Kristine M. Safford and Henry E. Rice).



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