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Doody's Review ServiceReviewer: Alvin Telser, PhD (Northwestern University Feinberg School of Medicine)
Description: This is a methods book that describes how to place about 15 different cell types into tissue culture and how to create cell lines from them. The majority of cell types described originate from human tissues.
Purpose: The purpose is to provide the experienced researcher with the details of techniques and procedures for creating a variety of immortalized cell cultures. These are very worthwhile objectives, and each chapter is well written to serve its intended purpose.
Audience: The book is written at an advanced level for experienced researchers in general and those scientists experienced in tissue culture. The intended target audience is appropriate for this book. Each chapter is written by one or a small group of experts in the field.
Features: This book contains an adequate number of tables and diagrams. The reproductions of the phase contrast light micrographs are rather poor, rendering them nearly useless as a guide to the user of the procedures. There are several very nice features in this volume. A comprehensive list of abbreviations appears at the beginning of the book. Each chapter has a list of special equipment or reagents and suppliers. Assays or other characteristics to confirm characteristics of the desired cell type are included in nearly every chapter. A separate chapter on tissue culture precautions is helpful. In general, the book is well produced.
Assessment: This well-done book should be useful to experienced researchers who may wish to establish immortalized cell lines. Individuals who may find this book useful would be well advised to purchase their own copy. Life science libraries should find that this will be a frequently used reference volume and should consider stocking it on their shelves.