Culture of Immortalized Cells / Edition 1

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Overview

Culture of Specialized Cells

Culture of Immortalized Cells

Edited by R. Ian Freshney and Mary G. Freshney

The ability to develop immortalized cells in vitro is a powerful tool for the biological investigator. Most cells can only be reproduced unchanged for a limited number of cell generations before the initial cell lines die. The immortalization of cells establishes continuous cell lines, playing an important role in the study of the biology of cell growth, differentiation, and senescence. It also increases our understanding of aberrations in proliferative and positional control in malignant cells.

Culture of Immortalized Cells provides the most up-to-date in vitro methods and safety procedures for the immortalization of primary cultures of various cell types. Coedited by one of the leading figures in the field, R. Ian Freshney, author of Culture of Animal Cells, Third Edition, Culture of Immortalized Cells is the latest volume in the critically acclaimed Culture of Specialized Cells Series. Preceding volumes in the series include Culture of Epithelial Cells and Culture of Hematopoietic Cells.

In this comprehensive handbook, leading experts offer the most current methods for the immortalization of cells, as well as specific guidelines for the immortalization of kidney, and thyroid epithelia, hepatocytes, fibroblasts, chondrocytes, lymphocytes, astrocytes, and human umbilical vein endothelium. Each method is accompanied by:
* Step-by-step protocols
* Detailed instructions for reagent preparation
* Current information on the latest specialized equipment and supplies

This concise bench-top manual is essential for any laboratory involved in establishing continuous cell lines from primary cultures. Investigators, laboratory technical staff, and students in cell biology, immunology, hematology, cancer biology, toxicology, and molecular biology, will find this contemporary guide indispensable to their research.

The book contains black-and-white illustrations.

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Editorial Reviews

Doody's Review Service
Reviewer: Alvin Telser, PhD (Northwestern University Feinberg School of Medicine)
Description: This is a methods book that describes how to place about 15 different cell types into tissue culture and how to create cell lines from them. The majority of cell types described originate from human tissues.
Purpose: The purpose is to provide the experienced researcher with the details of techniques and procedures for creating a variety of immortalized cell cultures. These are very worthwhile objectives, and each chapter is well written to serve its intended purpose.
Audience: The book is written at an advanced level for experienced researchers in general and those scientists experienced in tissue culture. The intended target audience is appropriate for this book. Each chapter is written by one or a small group of experts in the field.
Features: This book contains an adequate number of tables and diagrams. The reproductions of the phase contrast light micrographs are rather poor, rendering them nearly useless as a guide to the user of the procedures. There are several very nice features in this volume. A comprehensive list of abbreviations appears at the beginning of the book. Each chapter has a list of special equipment or reagents and suppliers. Assays or other characteristics to confirm characteristics of the desired cell type are included in nearly every chapter. A separate chapter on tissue culture precautions is helpful. In general, the book is well produced.
Assessment: This well-done book should be useful to experienced researchers who may wish to establish immortalized cell lines. Individuals who may find this book useful would be well advised to purchase their own copy. Life science libraries should find that this will be a frequently used reference volume and should consider stocking it on their shelves.
Alvin Telser
This is a methods book that describes how to place about 15 different cell types into tissue culture and how to create cell lines from them. The majority of cell types described originate from human tissues. The purpose is to provide the experienced researcher with the details of techniques and procedures for creating a variety of immortalized cell cultures. These are very worthwhile objectives, and each chapter is well written to serve its intended purpose. The book is written at an advanced level for experienced researchers in general and those scientists experienced in tissue culture. The intended target audience is appropriate for this book. Each chapter is written by one or a small group of experts in the field. This book contains an adequate number of tables and diagrams. The reproductions of the phase contrast light micrographs are rather poor, rendering them nearly useless as a guide to the user of the procedures. There are several very nice features in this volume. A comprehensive list of abbreviations appears at the beginning of the book. Each chapter has a list of special equipment or reagents and suppliers. Assays or other characteristics to confirm characteristics of the desired cell type are included in nearly every chapter. A separate chapter on tissue culture precautions is helpful. In general, the book is well produced. This well-done book should be useful to experienced researchers who may wish to establish immortalized cell lines. Individuals who may find this book useful would be well advised to purchase their own copy. Life science libraries should find that this will be a frequently used reference volume and should consider stocking it on their shelves.
Booknews
The development of immortalized cells in vitro has bypassed the problem of cell-line evolution that limited long-term research into the biology of cell growth, differentiation, and senescence. Veterans explain the methods and safety procedures for immortalizing several types of human and animal cells, including fibroblasts, epidermal keratinocytes, bronchial epithelial, hepatocytes, mesothelial, B-lymphocytes, astrocytes, and glial. The step-by-step protocols and procedures make the volume a handy laboratory manual. Annotation c. Book News, Inc., Portland, OR (booknews.com)

4 Stars! from Doody
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Product Details

  • ISBN-13: 9780471121343
  • Publisher: Wiley
  • Publication date: 4/15/1996
  • Series: Culture of Specialized Cells Series , #4
  • Edition number: 1
  • Pages: 416
  • Product dimensions: 6.93 (w) x 10.00 (h) x 0.76 (d)

Table of Contents

Partial table of contents:

Human Keratinocyte Immortalization: Genetic Basis and Role in Squamous Cell Carcinoma Development (E. Parkinson).

Safety Procedures (J. Caputo).

Mapping Human Senescence Genes Using Interspecific Monochromosome Transfer (R. Newbold & A. Cuthbert).

Development of Immortal Human Fibroblast Cell Lines (L. Mayne, et al.).

Immortalization of Human Bronchial Epithelial Cells (R. De Silva, et al.).

Thyroid Epithelium (D. Wynford-Thomas).

B-Lymphocytes (B. Bolton & N. Spurr).

Megakaryocyte Cell Lines from Transgenic Mice (K. Ravid).

List of Suppliers.

Index.

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