Culturing Nerve Cells / Edition 2

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Overview

Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms,from when the cells are first plated through the various phases of their development.Contributors : Janet Alder, Hannelore Asmussen, Gerard Bain, Gary Banker, Robert W. Baughman, Richard P. Bunge, Ann Marie Craig, Matthew E. Cunningham, Dominique Debanne, Stephen E. Farinelli, Michael F. A. Finley,Gerald D. Fishbach, Beat H. Gähwiler, W.-Q. Gao, Daniel J. Goldberg, Kimberly Goslin, David I.

Gottlieb, Lloyd A. Greene, Mary Beth Hatten, Dennis Higgins, James E. Huettner, Kenneth A. Jones,Naomi Kleitman, Raul Krauss, Ronald M. Lindsay, Nagesh K. Mahanthappa, Carol A. Mason, Margot Mayer-Pröschel, R. Anne McKinney, Mary E. Morrison, Mark Noble, David S. Park, Paul H. Patterson,Mu-ming Poo, Richard T. Robertson, Samuel Schacher, Michael M. Segal, Carolyn L. Smith, Nacira Tabti, Scott M. Thompson, Roseann Ventimiglia, Ginger S. Withers, Patrick M. Wood, Min Yao.

Disc. culturing of specific cell types, pros/cons of various systems, detailed protocols & descriptions.

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Editorial Reviews

Booknews
Robinson (history, U. of Southern California) details the moderate and the radical factions of the Korean nationalism movement, the controversies within and between the two groups, and the general direction of the movement during the period. He focuses on the rise of the moderate group and analyzes the origins and elaboration of its ideology as well as its major programs: the Korean Production, University, and Language movements. Inaugurating a new series, this lucid (and surprisingly lively) manual describes complete recipes and protocols for biological scientists, particularly neurobiologists, who want to learn how to grow neurons in culture. Following introductory chapters that essentially comprise a brief course on the basics of culturing all types of nerve cells, some 20 experts describe the culture systems that they have developed, including the details of each protocol as well as non-standard tricks and troubleshooting techniques. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9780262024389
  • Publisher: MIT Press
  • Publication date: 9/11/1998
  • Series: Cellular and Molecular Neuroscience Series
  • Edition description: second edition
  • Edition number: 2
  • Pages: 678
  • Sales rank: 1,142,922
  • Product dimensions: 7.00 (w) x 10.00 (h) x 1.65 (d)

Table of Contents

Foreword
Contributors
1 Getting Started 3
2 Types of Nerve Cell Cultures, Their Advantages and Limitations 11
3 Primary Dissociated Cell Cultures 37
4 Transfecting Cultured Neurons 79
5 Characterizing and Studying Neuronal Cultures 113
Choosing the Right System 155
6 Culture and Experimental Use of the PC12 Rat Pheochromocytoma Cell Line 161
7 Neuronlike Cells Derived in Culture from P19 Embryonal Carcinoma and Embryonic Stem Cells 189
8 Culturing the Large Neurons of Aplysia 213
9 Culturing Spinal Neurons and Muscle Cells from Xenopus Embryos 237
10 Cultures from Chick Peripheral Ganglia 261
11 Culturing Mammalian Sympathoadrenal Derivatives 289
12 Mass Cultures and Microlslands of Neurons from Postnatal Rat Brain 309
13 Rat Hippocampal Neurons in Low-Density Culture 339
14 Rat Striatal Neurons in Low-Density, Serum-Free Culture 371
15 Cell Culture of Cholinergic and Cholinoceptive Neurons from the Medial Habenula 395
16 The Cerebellum: Purification and Coculture of Identified Cell Populations 419
17 Organotypic Slice Cultures of Neural Tissue 461
18 Culture of Astrocytes, Oligodendrocytes, and O-2A Progenitor Cells 499
19 Tissue Culture Methods for the Study of Myelination 545
References 595
Index 659
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