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Scientists across disciplines have increasingly come to recognize the power of the protein. Current Protocols in Protein Science, a two-volume looseleaf manual, was developed in response to this revitalized interest and provides the most comprehensive collection of expert protein methods available. The publication covers both basic and advanced methods used in protein purification, characterization, and analysis as well as post-translational modification and structural analysis. More than 800 basic, support and alternate protocols have been carefully chosen for maximum applicability. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Quarterly updates, which are filed into the looseleaf, keep the set current with the latest developments in protein science methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Human Genetics, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.
Strategies of Protein Purification and Characterization.
Detection and Assay Methods.
Extraction, Stabilization & Concentration.
Production of Recombinant Proteins.
Purification of Recombinant Proteins.
Characterization of Recombinant Proteins.
Conventional Chromatographic Separations.
Post-Translational Modification: Glycosylation.
Post-Translational Modification: Phosphorylation and Phosphatases.
Post-Translational Modification: Specialized Approaches.
Chemical Modification of Proteins.
Preparation and Handling of Peptides.
Identification of Protein-Ligand Interactions.