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Doody's Review ServiceReviewer: Jed N. Lampe, Ph.D.(University of Kansas Medical Center)
Description: With this third edition, the authors and contributors continue to provide what has become a tour de force of bench top manuals for the cytochrome P450 field. Building on the success of the first two editions, this book expands and diversifies a set of well-established, validated protocols for the production and analysis of cytochrome P450 enzymes (CYPs) and their diverse metabolic products.
Purpose: The authors focus on high-throughput techniques for the examination of multiple CYPs in both in vitro and in vivo models, which is a notable expansion and extension of the fundamental techniques established in the earlier two editions.
Audience: Although predominantly useful for an industrial audience due to the high-throughput nature of most of the techniques, this book should also prove of interest to graduate students and postdoctoral researchers who are new to the field of P450 science, or who would like to expand their research directions.
Features: The highlight of this edition, as well as the previous editions, is the step-by-step protocols with extensive troubleshooting notes at the end of each chapter. These are augmented with selected and appropriate color illustrations that enhance readers' understanding of each technique.
Assessment: This stands alone as the premier book for this field, which is sorely lacking in publications of this type. Researchers in the P450 field will find this to be a useful bench top guide that will provide them with the tools to explore and understand CYPs, both in vitro and in vivo.