Cytotoxic Drug Resistance Mechanisms / Edition 1

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Overview

Leading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. The prools contain detailed instructions and extensive troubleshooting tips that allow researchers effectively to study a wide variety of drug resistance mechanisms, including aspects of drug-induced cell death, drug uptake/efflux, drug metabolism, and DNA repair. Each method is designed to help identify the correlation between molecular and biochemical data and the clinical responses of the patient. Cytotoxic Drug Resistance Mechanisms illuminates all the clinically relevant mechanisms used as markers of the biological response to anticancer biotherapeutics today.

The book contains black-and-white illustrations.

Leading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. The protocols contain detailed instructions and extensive troubleshooting tips that allow researchers effectively to study a wide variety of drug resistance mechanisms, including aspects of drug-induced cell death, drug uptake/efflux, drug metabolism, and DNA repair. Each method is designed to help identify the correlation between molecular and biochemical data and the clinical responses of the patient. Cytotoxic Drug Resistance Mechanisms illuminates all the clinically relevant mechanisms used as markers of the biological response to anticancer biotherapeutics today.

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Editorial Reviews

Raymond Bergan
This is a guidebook for conducting investigations on the effect of drugs on cancer cells. The purpose is to provide practical research protocols for researchers working in the field of resistance to anti-cancer agents. This book serves as a guidepost for the basic scientist, providing practical and specific protocols for the conduct of critical, technically demanding assays. In addition, it is a useful reference manual for clinical investigators, serving to provide a broad overview of specific assay types and their applicability within the context of clinical trials. All individuals involved in the field of drug development and drug resistance would benefit from this book, which is written and edited by a panel of well respected and accomplished individuals. Contained within individual chapters of this book are concise and technical overviews of assay methodologies and their relationship to underlying cancer cell biology and drug resistance. The format provides for rapid access to step-by-step protocols, followed by a pertinent discussion of both the strengths and limitations of individual approaches. This timely book will greatly benefit a wide array of individuals interested in cancer therapeutics, at both the clinical and basic science level. The editors and contributors did a good job of concisely presenting otherwise disparate methodologies in the context of a broad research field with the uniting purpose of understanding the biology underlying resistance to cancer chemotherapy. This well-chosen selection of methodologies and techniques of direct applicability to the field of drug resistance will serve as a practical way to unite a field encompassing a wide variety ofdisciplines.
Booknews
Describes methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. Review articles explain drug resistance methodology, and protocols contain detailed instructions and troubleshooting tips that allow researchers to study a variety of drug resistance mechanisms, including aspects of drug-induced cell death, drug uptake/efflux, drug metabolism, and DNA repair. Each method is designed to help identify the correlation between molecular and biochemical data and the clinical responses of the patient. Annotation c. by Book News, Inc., Portland, Or.
Doody's Review Service
Reviewer: Raymond C. Bergan, MD (Northwestern University Feinberg School of Medicine)
Description: This is a guidebook for conducting investigations on the effect of drugs on cancer cells.
Purpose: The purpose is to provide practical research protocols for researchers working in the field of resistance to anti-cancer agents.
Audience: This book serves as a guidepost for the basic scientist, providing practical and specific protocols for the conduct of critical, technically demanding assays. In addition, it is a useful reference manual for clinical investigators, serving to provide a broad overview of specific assay types and their applicability within the context of clinical trials. All individuals involved in the field of drug development and drug resistance would benefit from this book, which is written and edited by a panel of well respected and accomplished individuals.
Features: Contained within individual chapters of this book are concise and technical overviews of assay methodologies and their relationship to underlying cancer cell biology and drug resistance. The format provides for rapid access to step-by-step protocols, followed by a pertinent discussion of both the strengths and limitations of individual approaches.
Assessment: This timely book will greatly benefit a wide array of individuals interested in cancer therapeutics, at both the clinical and basic science level. The editors and contributors did a good job of concisely presenting otherwise disparate methodologies in the context of a broad research field with the uniting purpose of understanding the biology underlying resistance to cancer chemotherapy. This well-chosen selection of methodologies and techniques of direct applicability to the field of drug resistance will serve as a practical way to unite a field encompassing a wide variety of disciplines.

4 Stars! from Doody
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Product Details

  • ISBN-13: 9781617370939
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/9/2010
  • Series: Methods in Molecular Medicine Series , #28
  • Edition description: Softcover reprint of hardcover 1st ed. 1999
  • Edition number: 1
  • Pages: 238
  • Product dimensions: 0.57 (w) x 6.00 (h) x 9.00 (d)

Table of Contents

Drug Resistance: The Clinical Perspective
D. Alan Anthoney and Stanley B. Kaye

Cell Sensitivity Assays: Clonogenic Assay
Jane A. Plumb

Cell Sensitivity Assays: The MTT Assay
Jane A. Plumb

Cell Sensitivity Assays: Detection of Apoptotic Cells In Vitro Using the TUNEL Assay
Neil A. Jones and Caroline Dive

Analysis of Apoptosis in Tissue Sections
Vicki Save, Philip J. Coates, and Peter A. Hall

Measurement of P-glycoprotein Function
Henk J. Broxterman

Measuring MDR-1 by Quantitative RT-PCR
Susan E. Bates, Zhirong Zhan, Joanna Regis, and Erick Gamelin

Microtiter Plate Technique for the Measurement of Glutathione in Fresh and Cryopreserved Lymphoblasts Using the Enzyme Recycling Method
Pamela R. Kearns and Andrew G. Hall

Measurement of Reduced Glutathione Using High Pressure Liquid Chromatography
Linda A. Hogarth, Celia M. A. Rabello, and Andrew G. Hall

Topoisomerase I and II Activity Assays
Philippe Pourquier, Glenda Kohlhagen, Li-Ming Ueng, and Yves Pommier

5-Fluorouracil Metabolizing Enzymes
Howard L. McLeod, Lesley H. Milne, and Stephen J. Johnston

Measuring DNA Adducts by Immunoassay (ELISA)
Michael J. Tilby

Measuring Drug-DNA Adducts in Individual Cells
Adrian J. Frank

Measurement of Drug-Induced DNA Interstrand Crosslinking Using the Single Cell Electrophoresis (Comet) Assay
Victoria J. Spanswick, Janet M. Hartley, Timothy H. Ward, and John A. Hartley

PCR Analysis of Microsatellite Instability
Gillian L. Hirst

O 6-Alkylguanine-DNA Alkyltransferase Assay
Amanda J. Watson and Geoffrey P. Margison

Analysis of the p53 Status of Tumors: An Overview of Methods
Jonas Bergh

Bcl-2 Family Immunohishemistry
Lloyd R. Kelland and Philip J. Beale

Genetic Analysis of Drug Resistance by Fluorescence In Situ Hybridization
W. Nicol Keith

Genetic Analysis of Drug Resistance by ReverseIn Situ Hybridization
W. Nicol Keith

Index

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