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Cytotoxic Drug Resistance Mechanisms / Edition 1
     

Cytotoxic Drug Resistance Mechanisms / Edition 1

by Robert Brown, Uta B?ger-Brown
 

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ISBN-10: 1617370932

ISBN-13: 9781617370939

Pub. Date: 11/09/2010

Publisher: Springer-Verlag New York, LLC

Leading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. The protocols contain detailed instructions and extensive troubleshooting tips that allow researchers effectively to study a wide variety of drug resistance mechanisms, including aspects of

Overview

Leading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. The protocols contain detailed instructions and extensive troubleshooting tips that allow researchers effectively to study a wide variety of drug resistance mechanisms, including aspects of drug-induced cell death, drug uptake/efflux, drug metabolism, and DNA repair. Each method is designed to help identify the correlation between molecular and biochemical data and the clinical responses of the patient. Cytotoxic Drug Resistance Mechanisms illuminates all the clinically relevant mechanisms used as markers of the biological response to anticancer biotherapeutics today.

Product Details

ISBN-13:
9781617370939
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/09/2010
Series:
Methods in Molecular Medicine Series , #28
Edition description:
Softcover reprint of hardcover 1st ed. 1999
Pages:
238
Product dimensions:
5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

Drug Resistance: The Clinical Perspective
D. Alan Anthoney and Stanley B. Kaye

Cell Sensitivity Assays: Clonogenic Assay
Jane A. Plumb

Cell Sensitivity Assays: The MTT Assay
Jane A. Plumb

Cell Sensitivity Assays: Detection of Apoptotic Cells In Vitro Using the TUNEL Assay
Neil A. Jones and Caroline Dive

Analysis of Apoptosis in Tissue Sections
Vicki Save, Philip J. Coates, and Peter A. Hall

Measurement of P-glycoprotein Function
Henk J. Broxterman

Measuring MDR-1 by Quantitative RT-PCR
Susan E. Bates, Zhirong Zhan, Joanna Regis, and Erick Gamelin

Microtiter Plate Technique for the Measurement of Glutathione in Fresh and Cryopreserved Lymphoblasts Using the Enzyme Recycling Method
Pamela R. Kearns and Andrew G. Hall

Measurement of Reduced Glutathione Using High Pressure Liquid Chromatography
Linda A. Hogarth, Celia M. A. Rabello, and Andrew G. Hall

Topoisomerase I and II Activity Assays
Philippe Pourquier, Glenda Kohlhagen, Li-Ming Ueng, and Yves Pommier

5-Fluorouracil Metabolizing Enzymes
Howard L. McLeod, Lesley H. Milne, and Stephen J. Johnston

Measuring DNA Adducts by Immunoassay (ELISA)
Michael J. Tilby

Measuring Drug-DNA Adducts in Individual Cells
Adrian J. Frank

Measurement of Drug-Induced DNA Interstrand Crosslinking Using the Single Cell Electrophoresis (Comet) Assay
Victoria J. Spanswick, Janet M. Hartley, Timothy H. Ward, and John A. Hartley

PCR Analysis of Microsatellite Instability
Gillian L. Hirst

O 6-Alkylguanine-DNA Alkyltransferase Assay
Amanda J. Watson and Geoffrey P. Margison

Analysis of the p53 Status of Tumors: An Overview of Methods
Jonas Bergh

Bcl-2 Family Immunohishemistry
Lloyd R. Kelland and Philip J. Beale

Genetic Analysis of Drug Resistance by Fluorescence In Situ Hybridization
W. Nicol Keith

Genetic Analysis of Drug Resistance by ReverseIn Situ Hybridization
W. Nicol Keith

Index

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