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Doody's Review ServiceReviewer: Alvin Telser, PhD (Northwestern University Feinberg School of Medicine)
Description: This book in the Methods in Molecular Medicine series covers dendritic cell protocols.
Purpose: The authors describe this book as a collection of techniques in isolating and culturing animal and human dendritic cells as well as a few chapters devoted to possible therapeutic uses of these important cells of the immune system. Inasmuch as this is a book of techniques in a relatively new area of research of the immune system, the purposes of the book are worthwhile and are well attained.
Audience: "The book could be used effectively by experienced or beginning researchers in the field since the protocols are written clearly and concisely. There are 38 brief chapters in this book by over 70 authors, who are among the most experienced and knowledgeable researchers in the field. "
Features: Among the many experimental protocols described in this book are methods for isolating, culturing, and establishing cell lines from many organs from murine and human sources. The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to study these cells involved in the first line of defense of the immune system. The book has a utilitarian look and feel, and although the illustrations are not a major feature of the book, they are of medium quality. There is an adequate index.
Assessment: This book is intended and written as a laboratory guide manual. It would be quite useful for anyone doing bench research on murine or human dendritic cells.