Dengue: Methods and Protocols

Overview

Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with ...

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Overview

Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.

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Product Details

  • ISBN-13: 9781493903474
  • Publisher: Springer New York
  • Publication date: 4/3/2014
  • Series: Methods in Molecular Biology Series, #1138
  • Edition description: 2014
  • Pages: 403
  • Product dimensions: 7.10 (w) x 10.10 (h) x 1.10 (d)

Table of Contents

Part I. Modern Quantitative Virological Methods

1. Dengue Virus Growth, Purification and Fluorescent Labeling

Summer Zhang, Kuan Rong Chan, Hwee Cheng Tan, and Eng Eong Ooi

2. Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique

Milly M. Choy and Duane J. Gubler

3. Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique

A. Ruklanthi de Alwis and Aravinda M. de Silva

4. Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy

Joanne L. Tan and Lok Shee Mei

5. Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion

Kaiting Ng and John E. Connolly

6. Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated

Neutralization and Enhancement of Dengue Virus Infection

Swati Mukherjee, Ted Pierson and Kimberly A. Dowd

7. Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging

Kah Hin Tan, Kitti Chan Wing Ki, Satoru Watanabe, Subhash G. Vasudevan and Manoj Krishnan

Part II. Reverse Genetic Systems to Study Virus Replication and Evolution

8. Development and Application of Dengue Virus Reverse Genetic Systems

Andrew Davidson

9. DENV4 Renilla Luciferase Reporter Replicons

Sofia Alcaraz-Estrada, Rosa Del Angel, and R. Padmanabhan

10. Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo

Recombination

Mark Manzano and R. Padmanabhan

11. Identification of Dengue-Specific Human Antibody Fragments Using Phage Display

Moon Y.F. Tay, Chin Chin Lee, Subhash G. Vasudevan and NicoleJ.Moreland

12. Next Generation Whole Genome Sequencing of Dengue Virus

Pauline Poh Kim Aw, Paola Florez de Sessions, Andreas Wilm, Long Truong Hoang, Niranjan Nagarajan, October M. Sessions, and Martin Lloyd Hibberd

Part III. Modern Methods to Study Conformation of RNA Proteins and Their Molecular Interactions

13. MPGAfold in Dengue Secondary Structure Prediction

Wojciech K. Kasprzak and Bruce A. Shapiro

14. Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE

Joanna Sztuba-Solinska and Stuart F.J. Le Grice

15. Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5

Kyung H. Choi and Marc Morais

16. Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry

A.M. Ward, J. Gunaratne, M. A. Garcia-Blanco

17. Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore

Yau Yin Hoe and Susana Geifman Shochat

18. Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation

Nicholas J. Barrows, Sharon F. Jamison, Shelton S. Bradrick, Caroline Le Sommer, So Young Kim, James Pearson, and Mariano A. Garcia-Blanco

19. Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import

Johanna E. Fraser, Stephen M. Rawlinson, Chunxiao Wang, David A. Jans, and Kylie M. Wagstaff

Part IV. In vitro Enzyme Assays for Antiviral Screening

20. Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening

Mark Manzano, Janak Padia, and R. Padmanabhan

21. Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors

Huiguo Lai, Tadahisa Teramoto and Radhakrishnan Padmanabhan

22. Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase

Siwaporn Boonyasuppayakorn and Radhakrishnan Padmanabhan

Part V. Dengue Animal Model for Vaccines and Antivirals

23. Animal Models in Dengue

Emily Plummer and Sujan Shresta

24. Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model

Satoru Watanabe and Subhash G. Vasudevan

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