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Introduction: Why study cardiovascular development? Burggren and Keller; Part I. Molecular Cellular and Integrative Mechanisms: Determining Cardiovascular Development: 1. Genetic dissection of heart development Chen and Fishman; 2. Cardiac membrane characteristics Mahony; 3. Development of the myocardial contractile system Murphy; 4. Vasculogenesis and angiogenesis of the developing heart Tomanek and Ratajska; 5. Extracellular matrix maturation and morphogenesis Carver, Terracio and Borg; 6. Endothelial maturation Wong; 7. Embryonic cardiovascular function, coupling and maturation: a species view Keller; 8. Hormonal systems regulating the cardiovascular system Nakazawa and Kajio; Part II. Species Diversity in Cardiovascular Development: 9. Evolution of cardiovascular systems: insights into ontogeny Farrell; 10. Morphogenesis of vertebrate hearts Icardo; 11. Invertebrate cardiovascular development McMahon, Bourne and Chu; 12. Piscine cardiovascular development Rombough; 13. Amphibian cardiovascular development Burggren and Fritsche; 14. Reptilian cardiovascular development Warburton; 15. Avian cardiovascular development Tazawa and Hou; 16. Mammalian cardiovascular development Thornburg, Giraud, Reller and Morton; Part III. Environment and Disease in Cardiovascular Development: 17. Oxygen, temperature and pH influences on the development of non-mammalian embryos and larvae Pelster; 18. Modelling gas exchange in embryos, larvae and fetuses Pinder; 19. Principles of abnormal cardiac development Gittenberger-de Groot; 20. In utero and postnatal interventions in cardiovascular malformations Reddy and Hanley; 21. Insights into the future care of congenital cardiovascular malformations Clark; 22. Future directions in developmental cardiovascular sciences Keller and Burggren.