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Diabetes Mellitus / Edition 1

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Leading researchers and clinical investigators describe their best cutting-edge techniques for studying, at both the molecular and biochemical levels, the defects in insulin production and action associated with diabetes. Written in step-by-step detail to ensure ready reproducibility and robust results, these techniques allow investigators, both novices and those already active in the field, to study every major facet of insulin production and action. Each prool includes an introduction to the technique, an explanation of its application, and a list of materials. Practical notes discuss how to avoid pitfalls, as well as how to adapt the methods to your own research.
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Editorial Reviews

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"This book provides all the critical tools needed for the productive study of diabetes mellitus today." - Clinical Laboratory International

"Each chapter/prool is well referenced and the book is indexed. Because Diabetes Mellitus: Methods and Prools provides all the critical tools needed for the productive study of diabetes mellitus, it is a necessary purchase for libraries supporting basic research programs that study the molecular and biochemical bases of diabetes mellitus." -E-STREAMS

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Product Details

  • ISBN-13: 9781588291486
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 2/4/2003
  • Series: Methods in Molecular Medicine Series , #83
  • Edition description: 2003
  • Edition number: 1
  • Pages: 192
  • Product dimensions: 6.00 (w) x 9.00 (h) x 0.63 (d)

Table of Contents

Part I. Insulin Production

Isolation of Islets of Langerhans from Rodent Pancreas Colleen B. Kelly, Libby A. Blair, John A. Corbett, and Anna L. Scarim

Purification of Rat Pancreatic b-Cells by Fluorescence-Activated Cell Sorting Geert Stangé, Mark Van De Casteele, and Harry Heimberg

Assessment of Insulin Secretion in the Mouse Marcela Brissova, Wendell E. Nicholson, Masakazu Shiota, and Alvin C. Powers

Detection of Insulin Production by Immunohishemistry Susan C. Campbell and Wendy M. Macfarlane

Quantification of the Level of Insulin Gene Expression Susan C. Campbell and Wendy M. Macfarlane

Chromatin Immunoprecipitation Using Isolated Islets of Langerhans Marcelina Párrizas, Sylvia F. Boj, Reini F. Luco, Miguel Angel Maestro, and Jorge Ferrer

Adenoviral Gene Transfer into b-Cell Lines Amber L. Mosley and Sabire Özcan

Utilization of NOD Mice in the Study of Type 1 Diabetes Dennis G. Karounos and Susan E. Goes

Part II. Insulin Action

Introduction of DNA into 3T3-L1 Adipocytes by Electroporation Shuichi Okada, Masatomo Mori, and Jeffrey E. Pessin

Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes Jaganathan Lakshmanan, Jeffrey S. Elmendorf, and Sabire Özcan

Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes Jeffrey S. Elmendorf

Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay John B. Knight and Ann Louise Olson

Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates Eileen L. Whiteman and Morris J. Birnbaum

Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity Li-Ping Wang and Scott A. Summers

Assaying AKT/ Protein Kinase B Activity Kyle L. Hoehn and Scott A. Summers

Measurements of Cellular Phosphoinositide Levels in 3T3-L1 Adipocytes Daryll B. DeWald

Measurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 Adipocytes Matthew J. Brady

Measurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal Muscle Joseph T. Brozinick, Jr.

Single Embryo Measurement of Basal- and Insulin-Stimulated Glucose Uptake Maggie M-Y. Chi and Kelle H. Moley

Immunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of Glucose Transporters Mary O. Carayannopoulos and Kelle H. Moley


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