Diabetes Mellitus / Edition 1

Diabetes Mellitus / Edition 1

by Sabire ?zcan
     
 

ISBN-10: 1588291480

ISBN-13: 9781588291486

Pub. Date: 02/04/2003

Publisher: Springer-Verlag New York, LLC

This book provides a state-of-the-art account of the experimental methodology for studying the molecular defects leading to diabetes mellitus, both at the molecular and biochemical levels. The chapters are divided into two sections, on on insulin production and the other on insulin action. The detailed experimental protocols presented, including notes of interest,

…  See more details below

Overview

This book provides a state-of-the-art account of the experimental methodology for studying the molecular defects leading to diabetes mellitus, both at the molecular and biochemical levels. The chapters are divided into two sections, on on insulin production and the other on insulin action. The detailed experimental protocols presented, including notes of interest, provide a very useful tool for basic and clinical researchers investigating and treating diabetes.

Product Details

ISBN-13:
9781588291486
Publisher:
Springer-Verlag New York, LLC
Publication date:
02/04/2003
Series:
Methods in Molecular Medicine Series, #83
Edition description:
2003
Pages:
192
Product dimensions:
5.98(w) x 9.02(h) x 0.02(d)

Table of Contents

Part I. Insulin Production

Isolation of Islets of Langerhans from Rodent Pancreas Colleen B. Kelly, Libby A. Blair, John A. Corbett, and Anna L. Scarim

Purification of Rat Pancreatic b-Cells by Fluorescence-Activated Cell Sorting Geert Stangé, Mark Van De Casteele, and Harry Heimberg

Assessment of Insulin Secretion in the Mouse Marcela Brissova, Wendell E. Nicholson, Masakazu Shiota, and Alvin C. Powers

Detection of Insulin Production by Immunohishemistry Susan C. Campbell and Wendy M. Macfarlane

Quantification of the Level of Insulin Gene Expression Susan C. Campbell and Wendy M. Macfarlane

Chromatin Immunoprecipitation Using Isolated Islets of Langerhans Marcelina Párrizas, Sylvia F. Boj, Reini F. Luco, Miguel Angel Maestro, and Jorge Ferrer

Adenoviral Gene Transfer into b-Cell Lines Amber L. Mosley and Sabire Özcan

Utilization of NOD Mice in the Study of Type 1 Diabetes Dennis G. Karounos and Susan E. Goes

Part II. Insulin Action

Introduction of DNA into 3T3-L1 Adipocytes by Electroporation Shuichi Okada, Masatomo Mori, and Jeffrey E. Pessin

Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes Jaganathan Lakshmanan, Jeffrey S. Elmendorf, and Sabire Özcan

Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes Jeffrey S. Elmendorf

Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay John B. Knight and Ann Louise Olson

Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates Eileen L. Whiteman and Morris J. Birnbaum

Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity Li-Ping Wang and Scott A. Summers

Assaying AKT/ Protein Kinase B Activity Kyle L. Hoehn and Scott A. Summers

Measurements of Cellular Phosphoinositide Levels in 3T3-L1 Adipocytes Daryll B. DeWald

Measurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 Adipocytes Matthew J. Brady

Measurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal Muscle Joseph T. Brozinick, Jr.

Single Embryo Measurement of Basal- and Insulin-Stimulated Glucose Uptake Maggie M-Y. Chi and Kelle H. Moley

Immunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of Glucose Transporters Mary O. Carayannopoulos and Kelle H. Moley

Index

Read More

Customer Reviews

Average Review:

Write a Review

and post it to your social network

     

Most Helpful Customer Reviews

See all customer reviews >