Diagnostic Virology Protocols / Edition 1

Diagnostic Virology Protocols / Edition 1

by John R. Stephenson
     
 

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ISBN-10: 0896034011

ISBN-13: 9780896034013

Pub. Date: 08/28/1998

Publisher: Springer-Verlag New York, LLC

Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on

Overview

Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular BiologyT series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.

Product Details

ISBN-13:
9780896034013
Publisher:
Springer-Verlag New York, LLC
Publication date:
08/28/1998
Series:
Methods in Molecular Medicine Series, #12
Edition description:
1998
Pages:
372
Product dimensions:
5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

Rapid Detection of Adenovirus from Fecal Specimens
Tanvir Tabish, Alan Warnes, and Stuart Clark

Alphaviruses
John T. Roehrig, Teresa M. Brown, Alison J. Johnson, Nick
Karabatsos, Denise A. Martin, Carl J. Mitchell, and Roger S. Nasci

Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR
Xi Jiang and David O. Matson

Enteroviruses and Rhinoviruses
Peter Muir

Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction
Dennis W. Trent, Gwong-Jen Chang, A. Vance Vorndam,
and Robert S. Lanciotti

Hepatitis Viruses
Girish J. Kotwal

Herpesviruses
Mark J. Espy, P. Shawn Mitchell, David H. Persing, and Thomas F. Smith

Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper
Sharon Cassol, Stanley Read, Bruce G. Weniger, Richard Pilon, Barbara Leung, and Theresa Mo

PCR for the Detection of Influenza Viruses in Clinical Material

Lyssaviruses: Special Emphasis on Rabies Virus
Hervé Bourhy

Methods for Detecting Anti-Measles, Mumps, and Rubella Virus Antibodies
Ilkka Julkunen, Irja Davidkin, and Christian Oker-Blom

Papillomaviruses
Sandra H. Kirk and David T. Y. Liu

Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization
Karen E. Hicks, Stuart Beard, Bernard J. Cohen, and Jonathan P. Clewley

Polioviruses: Concurrent Serotyping and Intratypic Differentiation of Polioviruses
David J. Wood

Poxviruses
Hermann Meyer, Susan L. Ropp, and Joseph J. Esposito

Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections
G. L. Toms

Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis
Lennart Svensson

Detection of PCR-Amplified Sandfly Fever Sicilian Virus RNAby Colorimetric Enzymatic Immunoassay
M. Sofi Ibrahim and Connie S. Schmaljohn

Time-Resolved Fluorescence
Pekka Halonen and Timo Lövgren

Enzyme-Linked Immunosorbent Assays
David M. Kemeny

Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology
Joakim Lundeberg, Jacob Odeberg, and Mathias Uhlén

Robotic Analyzers
Dale R. Pfost and Richard G. Keightley

The Detection of Enteroviruses in Water and Associated Materials Using the Polymerase Chain Reaction
Peter Wyn-Jones and Jane Sellwood

Recombinant Antigens in Viral Diagnosis
John R. Stephenson and Alan Warnes

NASBA: A Method for Nucleic Acid Diagnostics
Dianne van Strijp and Pierre van Aarle

Oligonucleotides
Stina Syrjänen

Index

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