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Diagnostic Virology Protocols / Edition 1

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Overview

In Diagnostic Virology Protocols, internationally recognized experts present cutting-edge techniques for detecting many of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies - PCR, RIA, ELISA, and latex-agglutination - that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory using only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. The book also includes a number of chapters concisely evaluating many emerging technologies and their associated instrumentation.

The book contains black-and-white illustrations.

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Editorial Reviews

Doody's Review Service
Reviewer: Shirley F. Reising, PhD (Children's Hospital Medical Center)
Description: This book provides detailed laboratory procedures for the detection of most human viral pathogens using the most up-to-date methodology. Consistent procedural formatting throughout aids the reader in understanding the technologies.
Purpose: The editors propose to assimilate into one book modern diagnostic technologies for the detection of most viral pathogens.
Audience: This book is well suited for use in progressive laboratories engaged in rapid, dynamic analysis of clinical material. The methods detailed will soon become the standard for detection of viruses. Thus, it is an invaluable source of information for clinical and non-clinical virologists and microbiologists.
Features: The reader is guided through the procedures by consistent use of detailed algorithms. Experts in the field present point-by-point protocols and provide explicit commentary gained from their own experience. These state-of-the-art techniques and protocols for rapid detection of viruses can be readily adapted into most laboratories. Procedures are presented for the detection of common families of viruses using polymerase chain reaction (PCR), reverse transcriptase/polymerase chain reaction (RT/PCR), RNA gel electrophoresis, PCR-enzyme immunoassay (PCR-EIA), and others. Additional applications such as quantitation of viral DNA, rapid screening of blood donors for Parvovirus B-19, and automated sequencing of HIV-1 from dried blood spots are also outlined. Chapters reviewing use of automation, recombinant antigens, and nucleic acid sequence-based amplification (NASBA), emerging technologies that can be utilized for specific and sensitive viral diagnosis, are included.
Assessment: This book is useful and informative and would be a good asset to most research laboratories. While very few clinical laboratories would run these assays routinely, useful information can still be gained. For those that do perform the assays, it is invaluable. The obvious hands-on bench experience of the authors' sets this book apart from other diagnostic virology books that outline current methodologies for viral diagnosis such as Lennette's Laboratory Diagnosis of Viral Infectionsor Desselberger's Medical Virology: A Practical Approach. This is evidenced by the authors' detailed, helpful notes included at the end of each chapter.
Shirley F. Reising
This book provides detailed laboratory procedures for the detection of most human viral pathogens using the most up-to-date methodology. Consistent procedural formatting throughout aids the reader in understanding the technologies. The editors propose to assimilate into one book modern diagnostic technologies for the detection of most viral pathogens. This book is well suited for use in progressive laboratories engaged in rapid, dynamic analysis of clinical material. The methods detailed will soon become the standard for detection of viruses. Thus, it is an invaluable source of information for clinical and non-clinical virologists and microbiologists. The reader is guided through the procedures by consistent use of detailed algorithms. Experts in the field present point-by-point protocols and provide explicit commentary gained from their own experience. These state-of-the-art techniques and protocols for rapid detection of viruses can be readily adapted into most laboratories. Procedures are presented for the detection of common families of viruses using polymerase chain reaction (PCR), reverse transcriptase/polymerase chain reaction (RT/PCR), RNA gel electrophoresis, PCR-enzyme immunoassay (PCR-EIA), and others. Additional applications such as quantitation of viral DNA, rapid screening of blood donors for Parvovirus B-19, and automated sequencing of HIV-1 from dried blood spots are also outlined. Chapters reviewing use of automation, recombinant antigens, and nucleic acid sequence-based amplification (NASBA), emerging technologies that can be utilized for specific and sensitive viral diagnosis, are included. This book is useful and informative and would be a good asset to mostresearch laboratories. While very few clinical laboratories would run these assays routinely, useful information can still be gained. For those that do perform the assays, it is invaluable. The obvious hands-on bench experience of the authors' sets this book apart from other diagnostic virology books that outline current methodologies for viral diagnosis such as Lennette's Laboratory Diagnosis of Viral Infectionsor Desselberger's Medical Virology: A Practical Approach. This is evidenced by the authors' detailed, helpful notes included at the end of each chapter.
Booknews
Comprises 26 contributions which present techniques for detecting many of the major viruses that afflict mankind. Representative paper topics include rapid detection of adenovirus from fecal specimens, entoroviruses and rhinoviruses, poxviruses, and robotic analyzers. Includes information on all the new technologies: PCR; RIA; ELISA; and latex-agglutination. The protocols are designed to be easily reproducible. Plastic comb bound. Annotation c. by Book News, Inc., Portland, Or.
From the Publisher
". . .an invaluable source of information for clinical and non-clinical virologists and microbiologists. . .would be a good asset to most research laboratories. . .For those that do perform these assays, it is invaluable."-Doody's Health Sciences Book Review Journal

3 Stars from Doody
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Product Details

  • ISBN-13: 9780896034013
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 8/28/1998
  • Series: Methods in Molecular Medicine Series , #12
  • Edition description: 1998
  • Edition number: 1
  • Pages: 372
  • Product dimensions: 0.86 (w) x 6.00 (h) x 9.00 (d)

Table of Contents

Preface
Contributors
1 Rapid Detection of Adenovirus from Fecal Specimens 1
2 Alphaviruses 7
3 Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR 19
4 Enteroviruses and Rhinoviruses 29
5 Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction 51
6 Hepatitis Viruses 63
7 Herpesviruses 89
8 Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper 103
9 PCR for the Detection of Influenza Viruses in Clinical Material 119
10 Lyssaviruses: Special Emphasis on Rabies Virus 129
11 Methods for Detecting Anti-Measles, Mumps, and Rubella Virus Antibodies 143
12 Papillomaviruses 159
13 Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization 173
14 Polioviruses: Concurrent Serotyping and Intratypic Differentiation of Polioviruses 189
15 Poxviruses 199
16 Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections 213
17 Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis 223
18 Detection of PCR-Amplified Sandfly Fever Sicilian Virus RNA by Colorimetric Enzymatic Immunoassay 231
19 Time-Resolved Fluorescence 245
20 Enzyme-Linked Immunosorbent Assays 257
21 Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology 277
22 Robotic Analyzers 287
23 The Detection of Enteroviruses in Water and Associated Materials Using the Polymerase Chain Reaction 301
24 Recombinant Antigens in Viral Diagnosis 315
25 NASBA: A Method for Nucleic Acid Diagnostics 331
26 Oligonucleotides 341
Index 367
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