Diagnostic Virology Protocols / Edition 1

Diagnostic Virology Protocols / Edition 1

by John R. Stephenson
     
 

ISBN-10: 0896034798

ISBN-13: 9780896034792

Pub. Date: 08/28/1998

Publisher: Springer-Verlag New York, LLC

In Diagnostic Virology Protocols, internationally recognized experts present cutting-edge techniques for detecting many of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies - PCR, RIA, ELISA, and…  See more details below

Overview

In Diagnostic Virology Protocols, internationally recognized experts present cutting-edge techniques for detecting many of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies - PCR, RIA, ELISA, and latex-agglutination - that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory using only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. The book also includes a number of chapters concisely evaluating many emerging technologies and their associated instrumentation.

Product Details

ISBN-13:
9780896034792
Publisher:
Springer-Verlag New York, LLC
Publication date:
08/28/1998
Series:
Methods in Molecular Medicine Series, #12
Edition description:
1998
Pages:
372
Product dimensions:
6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Preface
Contributors
1Rapid Detection of Adenovirus from Fecal Specimens1
2Alphaviruses7
3Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR19
4Enteroviruses and Rhinoviruses29
5Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction51
6Hepatitis Viruses63
7Herpesviruses89
8Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper103
9PCR for the Detection of Influenza Viruses in Clinical Material119
10Lyssaviruses: Special Emphasis on Rabies Virus129
11Methods for Detecting Anti-Measles, Mumps, and Rubella Virus Antibodies143
12Papillomaviruses159
13Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization173
14Polioviruses: Concurrent Serotyping and Intratypic Differentiation of Polioviruses189
15Poxviruses199
16Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections213
17Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis223
18Detection of PCR-Amplified Sandfly Fever Sicilian Virus RNA by Colorimetric Enzymatic Immunoassay231
19Time-Resolved Fluorescence245
20Enzyme-Linked Immunosorbent Assays257
21Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology277
22Robotic Analyzers287
23The Detection of Enteroviruses in Water and Associated Materials Using the Polymerase Chain Reaction301
24Recombinant Antigens in Viral Diagnosis315
25NASBA: A Method for Nucleic Acid Diagnostics331
26Oligonucleotides341
Index367

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