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Overview
The book contains black-and-white illustrations.
Editorial Reviews
Doody's Review Service
Reviewer: Eugene A Davidson, PhD (Georgetown University School of Medicine)Description: This is the second edition (first edition published in 1997) of a compilation of protocols for investigators working with differential display. Both theoretical and practical aspects are addressed; several practical applications are included.
Purpose: The goal is to provide a set of protocols for investigators wishing to use differential display methods for monitoring gene expression. There are competing methods, notably array technologies, and the differences/advantages are not discussed in detail. The goal is worthwhile, but overall not well achieved.
Audience: This is essentially a practical laboratory manual and as such is intended for the senior graduate student/research fellow/investigator. There is sufficient detail to allow most of the experiments to be executed. The editors have assembled a diverse and capable group of contributors.
Features: Given the current knowledge of the genome and the ability to measure gene expression in multiple ways, it is to be expected that investigators will want to follow the "performance" of one or more genes during a variety of cellular events. These include differentiation/development, malignant transformation, sequelae of infectious processes, etc. Although the use of microarrays is very popular, these are high cost items and not available to all laboratories. Thus, alternative approaches are desirable. This book offers a series of protocols aimed at providing the tools necessary to use differential display. In principle, messenger RNA can be prepared from the tissue of interest, a cDNA copy made and then specific interrogation made of that population by PCR or other techniques. The initial chapter provides a theoretical discussion of global gene display addressing issues such as coverage of the chromosome when targets are not explicit. Subsequent chapters are laboratory oriented and include methods for automation, use of ordered displays, and PCR methods designed for this approach. The final section includes several explicit studies such as detection of genes expressed in prion disease, ovulation, and those related to p53. Each chapter has a short reference list to complement the detailed instructions. As a second edition, there is some new material. However, some sections are not very successful. Thus, the discussion of one new gene possibly identified in prion disease belongs in the primary literature awaiting confirmation. The automated procedure described is very challenging experimentally and, as with other chapters, insufficiently describes expected pitfalls and experimental snags. Investigators needing to use differential display will find useful, albeit limited, material.
Assessment: As a second edition, this book does not add very much. It is particularly important for a book of laboratory protocols to include caveats else the inexperienced investigator may be left at sea. At the same time, the experienced laboratorian is aware of the issues. The book seems cobbled together rather than tightly organized.
Philip M. Sass
This is a well thought-out and presented book that covers one of the most important molecular biology technologies developed, differential display. It is intended to provide an authoritative treatise on differential display so that researchers who are either unfamiliar with or are having problems can take advantage of the technology. The protocols that are included are well described and should be easy to follow for those with a basic grasp of molecular biology. The book is organized into several parts, including a basic methods section in the beginning of the book, which is followed by a series of parts that describe the application of differential display to various biological questions. These parts include sections on cloning family-specific genes and those genes expressed during normal development and during disease development. Furthermore, there are many helpful sections that follow each of the chapters that make the process of trouble-shooting fairly straightforward. I would highly recommend this book to libraries and to individuals who want to apply differential display technology to their research.Booknews
Twenty-four contributions describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as a strategy for identifying and cloning family- specific genes. The protocols provide examples in which differentially expressed genes were successfully identified in diverse biological systems, as well as new tools for studying how gene expression is regulated throughout the development of a living organism, and how the failure of this control mechanism leads to pathological complications. Plastic comb binding. Annotation c. by Book News, Inc., Portland, Or.5 Stars! from Doody
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Table of Contents
Part I. Methodologies
Global Analysis of Gene Expression by Differential Display: A Mathematical Model
Shitao Yang and Peng Liang
Automation of Fluorescent Differential Display With Digital Readout
Jonathan D. Meade, Yong-jig Cho, Jeffrey S. Fisher, Jamie C. Walden, Zhen Guo, and Peng Liang
Ordered Differential Display
Mikhail V. Matz and Ella A. Meleshkevitch
GeneCalling®: Transcript Profiling Coupled to a Gene Database Query
Richard A. Shimkets
High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR
Dana M. Walters and Pierre E. Rouvière
Vertical Arrays: Microarrays of Complex Mixtures of Nucleic Acids
Rosana Risques, Gaelle Rondeau, Martin Judex, Michael McClelland, and John Welsh
Automated Pattern Ranking in Differential Display Data Analysis
Tero Aittokallio, Pekka Ojala, Timo J. Nevalainen, and Olli S. Nevalainen
Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs
Ling Qin, Pjotr Prins, and Johannes Helder
Part II. Applications
Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepaellular Carcinoma
Dae-Ghon Kim
Identification of Disease Markers by Differential Display: Prion Disease
Michael Clinton, Gino Miele, Sunil Nandi, and Derek McBride
Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display
Yong-Jig Cho, Susanne Stein, Roger S. Jackson II, and Peng Liang
Identification of p53-Regulated Genes by the Method of Differential Display
Yunping Lin, Roger P. Leng, and Samuel Benchimol
Identification by Differential Display of IL-24 Aurine Loop Activated by Ras Oncogenes
Zhongjia Tan, Mai Wang, and Peng Liang
Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display
Lawrence L.Espey
Functional Analysis of Nocturnin: A Circadian Clock-Regulated Gene Identified by Differential Display
Julie E. Baggs and Carla B. Green
Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential Display
Mami Yamazaki and Kazuki Saito
Identification of Target Genes of a Yeast Transcriptional Repressor
Bernard Mai and Linda L. Breeden
Detection of an mRNA Polymorphism by Differential Display
Shan Liang, S. Paul Rossby, Peng Liang, Richard C. Shelton, D. Hal Manier, Amitabha Chakrabarti, and Fridolin Sulser
Silencing in Yeast: Identification of Clr4 Targets
Sergey V. Ivanov and Alla V. Ivanova
Identification of mRNA Bound to RNA Binding Proteins by Differential Display
Anne Carr-Schmid, Xinfu Jiao, and Megerditch Kiledjian
Index