Differential Display Methods and Protocols / Edition 2

Hardcover (Print)
Buy New
Buy New from BN.com
Used and New from Other Sellers
Used and New from Other Sellers
from $8.01
Usually ships in 1-2 business days
(Save 94%)
Other sellers (Hardcover)
  • All (12) from $8.01   
  • New (7) from $35.91   
  • Used (5) from $8.01   


Comprehensive review of this new technology, including methodology and practical applications. For investigators studying gene expression regulation. Plastic-comb, spiral binding.

The book contains black-and-white illustrations.

Read More Show Less

Editorial Reviews

Doody's Review Service
Reviewer: Eugene A Davidson, PhD (Georgetown University School of Medicine)
Description: This is the second edition (first edition published in 1997) of a compilation of protocols for investigators working with differential display. Both theoretical and practical aspects are addressed; several practical applications are included.
Purpose: The goal is to provide a set of protocols for investigators wishing to use differential display methods for monitoring gene expression. There are competing methods, notably array technologies, and the differences/advantages are not discussed in detail. The goal is worthwhile, but overall not well achieved.
Audience: This is essentially a practical laboratory manual and as such is intended for the senior graduate student/research fellow/investigator. There is sufficient detail to allow most of the experiments to be executed. The editors have assembled a diverse and capable group of contributors.
Features: Given the current knowledge of the genome and the ability to measure gene expression in multiple ways, it is to be expected that investigators will want to follow the "performance" of one or more genes during a variety of cellular events. These include differentiation/development, malignant transformation, sequelae of infectious processes, etc. Although the use of microarrays is very popular, these are high cost items and not available to all laboratories. Thus, alternative approaches are desirable. This book offers a series of protocols aimed at providing the tools necessary to use differential display. In principle, messenger RNA can be prepared from the tissue of interest, a cDNA copy made and then specific interrogation made of that population by PCR or other techniques. The initial chapter provides a theoretical discussion of global gene display addressing issues such as coverage of the chromosome when targets are not explicit. Subsequent chapters are laboratory oriented and include methods for automation, use of ordered displays, and PCR methods designed for this approach. The final section includes several explicit studies such as detection of genes expressed in prion disease, ovulation, and those related to p53. Each chapter has a short reference list to complement the detailed instructions. As a second edition, there is some new material. However, some sections are not very successful. Thus, the discussion of one new gene possibly identified in prion disease belongs in the primary literature awaiting confirmation. The automated procedure described is very challenging experimentally and, as with other chapters, insufficiently describes expected pitfalls and experimental snags. Investigators needing to use differential display will find useful, albeit limited, material.
Assessment: As a second edition, this book does not add very much. It is particularly important for a book of laboratory protocols to include caveats else the inexperienced investigator may be left at sea. At the same time, the experienced laboratorian is aware of the issues. The book seems cobbled together rather than tightly organized.
Philip M. Sass
This is a well thought-out and presented book that covers one of the most important molecular biology technologies developed, differential display. It is intended to provide an authoritative treatise on differential display so that researchers who are either unfamiliar with or are having problems can take advantage of the technology. The protocols that are included are well described and should be easy to follow for those with a basic grasp of molecular biology. The book is organized into several parts, including a basic methods section in the beginning of the book, which is followed by a series of parts that describe the application of differential display to various biological questions. These parts include sections on cloning family-specific genes and those genes expressed during normal development and during disease development. Furthermore, there are many helpful sections that follow each of the chapters that make the process of trouble-shooting fairly straightforward. I would highly recommend this book to libraries and to individuals who want to apply differential display technology to their research.
Twenty-four contributions describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as a strategy for identifying and cloning family- specific genes. The protocols provide examples in which differentially expressed genes were successfully identified in diverse biological systems, as well as new tools for studying how gene expression is regulated throughout the development of a living organism, and how the failure of this control mechanism leads to pathological complications. Plastic comb binding. Annotation c. by Book News, Inc., Portland, Or.

5 Stars! from Doody
Read More Show Less

Product Details

  • ISBN-13: 9781588293381
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 10/28/2005
  • Series: Methods in Molecular Biology Series , #317
  • Edition description: 2nd ed. 2006
  • Edition number: 2
  • Pages: 320
  • Product dimensions: 6.20 (w) x 9.30 (h) x 1.10 (d)

Table of Contents

Part I. Methodologies

Global Analysis of Gene Expression by Differential Display: A Mathematical Model
Shitao Yang and Peng Liang

Automation of Fluorescent Differential Display With Digital Readout
Jonathan D. Meade, Yong-jig Cho, Jeffrey S. Fisher, Jamie C. Walden, Zhen Guo, and Peng Liang

Ordered Differential Display
Mikhail V. Matz and Ella A. Meleshkevitch

GeneCalling®: Transcript Profiling Coupled to a Gene Database Query
Richard A. Shimkets

High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR
Dana M. Walters and Pierre E. Rouvière

Vertical Arrays: Microarrays of Complex Mixtures of Nucleic Acids
Rosana Risques, Gaelle Rondeau, Martin Judex, Michael McClelland, and John Welsh

Automated Pattern Ranking in Differential Display Data Analysis
Tero Aittokallio, Pekka Ojala, Timo J. Nevalainen, and Olli S. Nevalainen

Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs
Ling Qin, Pjotr Prins, and Johannes Helder

Part II. Applications

Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepaellular Carcinoma
Dae-Ghon Kim

Identification of Disease Markers by Differential Display: Prion Disease
Michael Clinton, Gino Miele, Sunil Nandi, and Derek McBride

Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display
Yong-Jig Cho, Susanne Stein, Roger S. Jackson II, and Peng Liang

Identification of p53-Regulated Genes by the Method of Differential Display
Yunping Lin, Roger P. Leng, and Samuel Benchimol

Identification by Differential Display of IL-24 Aurine Loop Activated by Ras Oncogenes
Zhongjia Tan, Mai Wang, and Peng Liang

Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display
Lawrence L.Espey

Functional Analysis of Nocturnin: A Circadian Clock-Regulated Gene Identified by Differential Display
Julie E. Baggs and Carla B. Green

Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential Display
Mami Yamazaki and Kazuki Saito

Identification of Target Genes of a Yeast Transcriptional Repressor
Bernard Mai and Linda L. Breeden

Detection of an mRNA Polymorphism by Differential Display
Shan Liang, S. Paul Rossby, Peng Liang, Richard C. Shelton, D. Hal Manier, Amitabha Chakrabarti, and Fridolin Sulser

Silencing in Yeast: Identification of Clr4 Targets
Sergey V. Ivanov and Alla V. Ivanova

Identification of mRNA Bound to RNA Binding Proteins by Differential Display
Anne Carr-Schmid, Xinfu Jiao, and Megerditch Kiledjian


Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)