Differential Display Methods and Protocols / Edition 2

by Peng Liang, Jonathan Meade, Arthur B. Pardee

Overview

Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an “open”-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms—from bacteria, fungi, insects, fish, plants, to mammals—even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious “cottage-industry” academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.

Product Details

ISBN-13:	9781588293381
Publisher:	Springer-Verlag New York, LLC
Publication date:	10/21/2005
Series:	Methods in Molecular Biology , #317
Edition description:	Second Edition 2006
Pages:	320
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

About the Author

Liang, Peng (Vanderbilt Cancer Center); Pardee, Arthur B. (Dana-Farber Cancer Institute)

The contributors represent the specialties of molecular biology, ocular oncology, cell biology, pathology, cancer genetics, pharmacology, and pediatric oncology. Most come from research institutes, cancer centers, and universities in six countries, including the U.S., Canada, and Japan. Institutions prominently represented include DNAX Research Institute, National Institutes of Health, Dana-Farber Cancer Institute, Vanderbilt Cancer Center, USC, Harvard, Dalhousie Univ, and Univ of Tokyo.

Table of Contents

Methodologies.- Global Analysis of Gene Expression by Differential Display.- Automation of Fluorescent Differential Display With Digital Readout.- Ordered Differential Display.- GeneCalling®.- High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR.- Vertical Arrays.- Automated Pattern Ranking in Differential Display Data Analysis.- Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs.- Applications.- Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepaellular Carcinoma.- Identification of Disease Markers by Differential Display.- Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display.- Identification of p53-Regulated Genes by the Method of Differential Display.- Identification by Differential Display of IL-24 Aurine Loop Activated by Ras Oncogenes.- Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display.- Functional Analysis of Nocturnin.- Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential Display.- Identification of Target Genes of a Yeast Transcriptional Repressor.- Detection of an mRNA Polymorphism by Differential Display.- Silencing in Yeast.- Identification of mRNA Bound to RNA Binding Proteins by Differential Display.

Editorial Reviews

5 Stars! from Doody

This is a well thought-out and presented book that covers one of the most important molecular biology technologies developed, differential display. It is intended to provide an authoritative treatise on differential display so that researchers who are either unfamiliar with or are having problems can take advantage of the technology. The protocols that are included are well described and should be easy to follow for those with a basic grasp of molecular biology. The book is organized into several parts, including a basic methods section in the beginning of the book, which is followed by a series of parts that describe the application of differential display to various biological questions. These parts include sections on cloning family-specific genes and those genes expressed during normal development and during disease development. Furthermore, there are many helpful sections that follow each of the chapters that make the process of trouble-shooting fairly straightforward. I would highly recommend this book to libraries and to individuals who want to apply differential display technology to their research.

Philip M. Sass

Twenty-four contributions describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as a strategy for identifying and cloning family- specific genes. The protocols provide examples in which differentially expressed genes were successfully identified in diverse biological systems, as well as new tools for studying how gene expression is regulated throughout the development of a living organism, and how the failure of this control mechanism leads to pathological complications. Plastic comb binding. Annotation c. by Book News, Inc., Portland, Or.

Booknews

Reviewer: Philip M. Sass, PhD (University of Illinois at Chicago College of Medicine)
Description: This is a well thought-out and presented book that covers one of the most important molecular biology technologies developed, differential display.
Purpose: It is intended to provide an authoritative treatise on differential display so that researchers who are either unfamiliar with or are having problems can take advantage of the technology.
Audience: The protocols that are included are well described and should be easy to follow for those with a basic grasp of molecular biology.
Features: The book is organized into several parts, including a basic methods section in the beginning of the book, which is followed by a series of parts that describe the application of differential display to various biological questions. These parts include sections on cloning family-specific genes and those genes expressed during normal development and during disease development. Furthermore, there are many helpful sections that follow each of the chapters that make the process of trouble-shooting fairly straightforward.
Assessment: I would highly recommend this book to libraries and to individuals who want to apply differential display technology to their research.

Doody's Review Service

"The variety of applications...is so broad that almost every newcomer...can use these prools as a starting point for a specific study...highly recommended for purchase by everyone interested in the comparative gene expression, and especially to newcomers to the field." -Trends in Biotechnology

From Reviews of the First Edition...

"...really helpful for all geneticists and cellular and molecular biologists...a precious book which should be in all hands!" -Cellular and Molecular Biology

"...For any would-be gene hunter thinking of or already practicing differential display, this book will provide an invaluable and long overdue guide to the technique." -Molecular Medicine

"....an ideal resource for any research group interested in using DD." -Microbiology Today

"...highly recommend[ed]...for purchase by everyone interested in the comparative gene expression,, and especially to newcomers to the field." -Trends in Biotechnology

From the Publisher