District Laboratory Practice in Tropical Countries, Part 2 / Edition 2

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This new edition includes an update on HIV disease/AIDS, recently developed HIV rapid tests to diagnose HIV infection and screen donor blood, and current information on antiretroviral drugs and the laboratory monitoring of antiretroviral therapy. Information on the epidemiology and laboratory investigation of other pathogens has also been brought up to date. Several new, rapid, simple to perform immunochromatographic tests to assist in the diagnosis of infectious diseases are described, including those for brucellosis, cholera, dengue, leptospirosis, syphilis and hepatitis. Recently developed lgM antibody tests to investigate typhoid fever are also described. The new classification of salmonellae has been introduced. Details of manufacturers and suppliers now include website information and e-mail addresses. The haematology and blood transfusion chapters have been updated, including a review of haemoglobin measurement methods in consideration of the high prevalence of anaemia in developing countries.

The book contains predominantly black-and-white illustrations, with some color illustrations.

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Editorial Reviews

From the Publisher
'The volume is packed with much valuable information, which is presented in a format that is readily readable. There are ample clear illustrations, tables and photographs to render the various information easy to digest. The authors have succeeded in producing a work that will fulfill an important need for developing countries. I highly recommend this book, with its Part I counterpart, to anyone with an interest in the practice of laboratory medicine.' Pathology

'… District Laboratory Practice in Tropical Countries sets the gold standard, and is an essential read and reference for anyone engaged in clinical laboratory practice in the tropics.' Tropical Doctor

From The Critics
Reviewer:Valerie L. Ng, PhD MD(Alameda County Medical Center/Highland Hospital)
Description:This is the second of a two-volume set that addresses practical issues related to microbiology, hematology, and transfusion medicine laboratory testing and clinical laboratory medicine practice.
Purpose:The purpose of the set is to make available -- at low cost -- good laboratory practice concepts, methods, and procedures for those working in tropical countries, "often in difficult and challenging situations.
Audience:The author intends this book for laboratorians working with limited means. This book, however, would be useful for laboratorians everywhere, as well as medical technology students, medical students, pathology and laboratory medicine residents in training and practicing pathologists.
Features:This is a condensed, concise, and highly informative book on recommended microbiology, hematology and transfusion medicine laboratory practice. It is amazingly complete -- ranging from preanalytical issues (specimen handling, volunteer blood donation) to detailed methods (including how to make Pasteur pipettes from glass tubing!) to clinical interpretation of relevant laboratory test results. A nice feature is the 20 pages of color photomicrographs (peripheral blood) and other illustrative plates (various microbiology tests -- bacterial colonies, rapid tests including membrane-based ones) which are of wonderfully high quality and educational value. I especially enjoyed the remarkably thorough 11 chapters covering all aspects of hematology -- anemia, hemoglobin variants, coagulation, leukemia, etc. -- in just 80 pages. Another nice feature is the appendixes -- appendix I contains recipes for bacterial culture media, and appendix II contains useful addresses for manufacturers and suppliers of laboratory reagents and supplies. The only drawback to this book is the glaring absence of information on topics covered in Part 1. After leafing through this book, I must admit that I began to consider replacing my many books on microbiology, hematology and transfusion medicine with just this volume, as well as to think about reviewing current practice in these areas in my own laboratory to identify those which could be performed at lower cost and labor, yet maintaining the same high level of quality!
Assessment:This is really a great book -- everything you need to know at your fingertips in just one book. Although developed as a low cost alternative for laboratories in tropical countries having limited resources, this book would be just as useful for any clinical laboratory anywhere.

5 Stars! from Doody
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Product Details

  • ISBN-13: 9780521676311
  • Publisher: Cambridge University Press
  • Publication date: 2/28/2006
  • Edition description: Revised Edition
  • Edition number: 2
  • Pages: 440
  • Sales rank: 1,568,415
  • Product dimensions: 7.68 (w) x 9.92 (h) x 0.83 (d)

Table of Contents

Preface; 7. Microbiological tests; 7.1 Microbiology practice and quality assurance in district laboratories; 7.2 Features and classification of micro-organisms of medical importance; 7.3 Microscopical techniques used in microbiology; 7.4 Culturing bacterial pathogens; 7.5 Biochemical tests to identify bacteria; 7.6 Examination of sputum; 7.7 Examination of throat and mouth specimens; 7.8 Examination of pus, ulcer material and skin specimens; 7.9 Examination of effusions; 7.10 Examination of urogenital specimens; 7.11 Examination of faecal specimens; 7.12 Examination of urine; 7.13 Examination of cerebrospinal fluid (c.s.f.); 7.14 Culturing blood; 7.15 Examination of semen; 7.16 Antimicrobial sensitivity testing; 7.17 Water-related disease and testing of water supplies; 7.18 Summary of the clinical and laboratory features of micro-organisms; Colour plate section; 8. Haematological tests; 8.1 Haematology in district laboratories and quality assurance; 8.2 Functions of blood, haematopoiesis and blood disorders; 8.3 Collection of blood; 8.4 Measurement of haemoglobin; 8.5 PCV and red cell indices; 8.6 Counting white cells and platelets; 8.7 Blood films; 8.8 Erythrocyte sedimentation rate; 8.9 Reticulocyte count. Methaemoglobin reduction test; 8.10 Investigation of sickle cell disease; 8.11 Investigation of bleeding disorders; 9. Blood transfusion tests; 9.1 Blood transfusion services at district level and quality assurance; 9.2 Blood donation and storage of blood; 9.3 Blood grouping; 9.4 Compatibility testing; recommended books; Appendix I. Preparation of reagents and culture media; Appendix II. Useful addresses; Index.
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